{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,2]],"date-time":"2026-09-02T23:01:58Z","timestamp":1788390118377,"version":"build-2803163510"},"reference-count":30,"publisher":"The Royal Society","issue":"7","license":[{"start":{"date-parts":[[2025,7,1]],"date-time":"2025-07-01T00:00:00Z","timestamp":1751328000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,7,1]],"date-time":"2025-07-01T00:00:00Z","timestamp":1751328000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100017899","name":"Masason Foundation","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100017899","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003806","name":"Hokuto Foundation for Bioscience","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100003806","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Keio University Academic Development Funds"},{"name":"Tsuruoka City"},{"name":"Chugai Foundation for Innovative Drug Discovery Science"},{"name":"Yamagata Prefecture"},{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["royalsocietypublishing.org"],"crossmark-restriction":true},"short-container-title":["Biol. Lett."],"published-print":{"date-parts":[[2025,7]]},"abstract":"<jats:p>\n                    Myxomycetes are unicellular amoebozoans that form fruiting bodies to reproduce, a process known as sporulation. In the model species\n                    <jats:italic toggle=\"yes\">Physarum polycephalum<\/jats:italic>\n                    , plasmodia form fruiting bodies only after several days of starvation followed by light exposure. It has long been assumed that the same starvation-plus-light trigger applies to the genus\n                    <jats:italic toggle=\"yes\">Physarum<\/jats:italic>\n                    . Recent observations of congeners that fail to sporulate under the same conditions have raised doubts about this assumption and prompted tentative taxonomic reconsideration. Because comparable starvation and light tests are rare for other species of\n                    <jats:italic toggle=\"yes\">Physarum<\/jats:italic>\n                    , their phenotypes and molecular mechanisms remain unclear. Consequently, we investigated\n                    <jats:italic toggle=\"yes\">Physarum rigidum<\/jats:italic>\n                    and\n                    <jats:italic toggle=\"yes\">Physarum roseum<\/jats:italic>\n                    under starvation and light conditions. Four of the six\n                    <jats:italic toggle=\"yes\">P. rigidum<\/jats:italic>\n                    plasmodia sporulated by day 6, whereas\n                    <jats:italic toggle=\"yes\">P. roseum<\/jats:italic>\n                    did not sporulate within 7 days. RNA-seq of\n                    <jats:italic toggle=\"yes\">P. roseum<\/jats:italic>\n                    across nutrient-rich\/starved and dark\/light conditions revealed differential expression was driven chiefly by nutrition; light caused only minor changes and did not elicit the transcriptional programme characteristic of\n                    <jats:italic toggle=\"yes\">P. polycephalum<\/jats:italic>\n                    sporulation. The photoreceptor genes that drive sporulation in\n                    <jats:italic toggle=\"yes\">P. polycephalum<\/jats:italic>\n                    were not detected in\n                    <jats:italic toggle=\"yes\">P. roseum<\/jats:italic>\n                    , and 92 candidate photoreceptor genes showed no significant regulation. These findings suggest that\n                    <jats:italic toggle=\"yes\">P. roseum<\/jats:italic>\n                    responds only minimally to light stimulation and that the starvation-plus-light trigger is not universally retained within the genus\n                    <jats:italic toggle=\"yes\">Physarum<\/jats:italic>\n                    .\n                  <\/jats:p>","DOI":"10.1098\/rsbl.2025.0215","type":"journal-article","created":{"date-parts":[[2025,7,30]],"date-time":"2025-07-30T10:16:12Z","timestamp":1753870572000},"update-policy":"https:\/\/doi.org\/10.1098\/crossmark-policy","source":"Crossref","is-referenced-by-count":3,"title":["Loss of the starvation-and-light fruitbody formation trigger in the myxomycete\n                    <i>Physarum roseum<\/i>"],"prefix":"10.1098","volume":"21","author":[{"given":"Mana","family":"Masui","sequence":"first","affiliation":[{"name":"Graduate School of Media and Governance, Keio University","place":["Fujisawa, Japan"]},{"name":"Institute for Advanced Biosciences, Keio University","place":["Tsuruoka, Japan"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0000-9253-8972","authenticated-orcid":true,"given":"Phillip K.","family":"Yamamoto","sequence":"additional","affiliation":[{"name":"Graduate School of Media and Governance, Keio University","place":["Fujisawa, Japan"]},{"name":"Institute for Advanced Biosciences, Keio University","place":["Tsuruoka, Japan"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5960-7956","authenticated-orcid":true,"given":"Nobuaki","family":"Kono","sequence":"additional","affiliation":[{"name":"Graduate School of Media and Governance, Keio University","place":["Fujisawa, Japan"]},{"name":"Institute for Advanced Biosciences, Keio University","place":["Tsuruoka, Japan"]},{"name":"Faculty of Environment and Information Studies, Keio University","place":["Fujisawa, Japan"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"175","published-online":{"date-parts":[[2025,7,30]]},"reference":[{"key":"e_1_3_8_2_2","doi-asserted-by":"publisher","DOI":"10.5943\/mycosphere\/3\/2\/3"},{"key":"e_1_3_8_3_2","doi-asserted-by":"publisher","DOI":"10.1508\/cytologia.15.194"},{"key":"e_1_3_8_4_2","volume-title":"Biology of the myxomycetes.","author":"Gray WD","year":"1968","unstructured":"Gray WD, Alexopoulos CJ. 1968 Biology of the myxomycetes. 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