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Lett."],"published-print":{"date-parts":[[2025,10]]},"abstract":"<jats:p>\n                    Sex-determining genes remain largely uncharacterized outside classical models in vertebrates and insects, leaving a gap in our understanding of their evolutionary emergence. The terrestrial isopod\n                    <jats:italic toggle=\"yes\">Armadillidium vulgare<\/jats:italic>\n                    provides an excellent model for investigating this question, as it presents multiple genetic sex determinants. Some lineages possess a masculinizing dominant allele at a locus called the \u2018\n                    <jats:italic toggle=\"yes\">M<\/jats:italic>\n                    gene\u2019, which is analogous to an XY system. This allele is hypothesized to have been selected due to the deficit of males caused by a non-Mendelian feminizing factor previously described. The existence of the\n                    <jats:italic toggle=\"yes\">M<\/jats:italic>\n                    gene was inferred from crosses carried out in the 1990s, but its molecular nature remains unresolved. Here, we conducted a genome-wide single-nucleotide polymorphisms analysis combining pooled sequencing of male and female progenies with sequencing of individual parents across two families. Bayesian estimation of haplotype frequencies in progenies pinpointed a candidate genomic region of approximately two megabases. Notably, this region contains the gene encoding the androgenic gland hormone, a protein involved in male sexual differentiation. Our findings lay the groundwork for functional investigations of the\n                    <jats:italic toggle=\"yes\">M<\/jats:italic>\n                    gene, offering novel insights into the dynamics of sex determination in terrestrial isopods and into the turnover of sex chromosomes in response to sex-ratio distortion.\n                  <\/jats:p>","DOI":"10.1098\/rsbl.2025.0287","type":"journal-article","created":{"date-parts":[[2025,10,21]],"date-time":"2025-10-21T23:05:32Z","timestamp":1761087932000},"update-policy":"https:\/\/doi.org\/10.1098\/crossmark-policy","source":"Crossref","is-referenced-by-count":0,"title":["Identification of a sex-determining region potentially involved in resolving genetic conflicts over sex ratio"],"prefix":"10.1098","volume":"21","author":[{"ORCID":"https:\/\/orcid.org\/0009-0004-5891-2990","authenticated-orcid":true,"given":"Baptiste","family":"Lh\u00e9raud","sequence":"first","affiliation":[{"name":"Laboratoire d'\u00c9cologie Microbienne (UMR 5557, UMR 1418), CNRS, Universit\u00e9 Claude Bernard Lyon 1, INRAE, VetAgro Sup, Villeurbanne 69622, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1900-7715","authenticated-orcid":true,"given":"Yann","family":"Dussert","sequence":"additional","affiliation":[{"name":"G\u00e9nomique M\u00e9tabolique, Genoscope, Institut Fran\u00e7ois Jacob, CEA, CNRS, Universit\u00e9 d\u2019\u00c9vry, Universit\u00e9 Paris-Saclay, \u00c9vry 91057, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mohamed Amine","family":"Chebbi","sequence":"additional","affiliation":[{"name":"Laboratoire \u00c9cologie et Biologie des Interactions (UMR 7267), CNRS, Universit\u00e9 de Poitiers, Poitiers 86000, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Isabelle","family":"Giraud","sequence":"additional","affiliation":[{"name":"Laboratoire \u00c9cologie et Biologie des Interactions (UMR 7267), CNRS, Universit\u00e9 de Poitiers, Poitiers 86000, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4997-735X","authenticated-orcid":true,"given":"Richard","family":"Cordaux","sequence":"additional","affiliation":[{"name":"Laboratoire \u00c9volution G\u00e9nomes Comportement \u00c9cologie (UMR 9191), CNRS, Universit\u00e9 Paris-Saclay, IRD, Gif-sur-Yvette 91190, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3356-7869","authenticated-orcid":true,"given":"Jean","family":"Peccoud","sequence":"additional","affiliation":[{"name":"Laboratoire \u00c9cologie et Biologie des Interactions (UMR 7267), CNRS, Universit\u00e9 de Poitiers, Poitiers 86000, France"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"175","published-online":{"date-parts":[[2025,10,22]]},"reference":[{"key":"e_1_3_8_2_2","doi-asserted-by":"publisher","DOI":"10.1093\/gbe\/evaa081"},{"key":"e_1_3_8_3_2","doi-asserted-by":"publisher","DOI":"10.1086\/283342"},{"key":"e_1_3_8_4_2","doi-asserted-by":"publisher","DOI":"10.1111\/nph.16002"},{"key":"e_1_3_8_5_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pgen.1002798"},{"key":"e_1_3_8_6_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41477-019-0489-6"},{"key":"e_1_3_8_7_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pbio.3000438"},{"key":"e_1_3_8_8_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.ecolsys.29.1.233"},{"key":"e_1_3_8_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.tree.2007.03.008"},{"key":"e_1_3_8_10_2","doi-asserted-by":"publisher","DOI":"10.1038\/hdy.2009.104"},{"key":"e_1_3_8_11_2","doi-asserted-by":"publisher","DOI":"10.1098\/rspb.2016.2806"},{"key":"e_1_3_8_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.tig.2011.05.002"},{"key":"e_1_3_8_13_2","doi-asserted-by":"publisher","DOI":"10.1093\/gbe\/evab121"},{"key":"e_1_3_8_14_2","doi-asserted-by":"publisher","DOI":"10.1098\/rspb.1998.0402"},{"key":"e_1_3_8_15_2","doi-asserted-by":"publisher","DOI":"10.1186\/1297-9686-16-1-57"},{"key":"e_1_3_8_16_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1608979113"},{"key":"e_1_3_8_17_2","doi-asserted-by":"publisher","DOI":"10.3390\/genes8070186"},{"key":"e_1_3_8_18_2","doi-asserted-by":"publisher","DOI":"10.1093\/genetics\/133.2.247"},{"key":"e_1_3_8_19_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ygcen.2004.05.003"},{"key":"e_1_3_8_20_2","doi-asserted-by":"publisher","DOI":"10.1002\/bies.950190508"},{"key":"e_1_3_8_21_2","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btp324"},{"key":"e_1_3_8_22_2","doi-asserted-by":"crossref","unstructured":"Lh\u00e9raud B Dussert Y Chebbi MA Giraud I Cordaux R Peccoud J. 2025 Sequence data from: Identification of a sex-determining region potentially involved in resolving genetic conflicts over sex-ratio. 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