{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,5]],"date-time":"2026-05-05T12:27:27Z","timestamp":1777984047568,"version":"3.51.4"},"reference-count":24,"publisher":"The Royal Society","issue":"3","license":[{"start":{"date-parts":[[2026,3,18]],"date-time":"2026-03-18T00:00:00Z","timestamp":1773792000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,3,18]],"date-time":"2026-03-18T00:00:00Z","timestamp":1773792000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000057","name":"National Institute of General Medical Sciences","doi-asserted-by":"publisher","award":["R01 GM130842-01"],"award-info":[{"award-number":["R01 GM130842-01"]}],"id":[{"id":"10.13039\/100000057","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000055","name":"National Institute on Deafness & Other Communication Disorders","doi-asserted-by":"publisher","award":["5F31DC020132"],"award-info":[{"award-number":["5F31DC020132"]}],"id":[{"id":"10.13039\/100000055","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,3,18]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>The difficulty of cryopreservation has long been a limitation of Drosophila melanogaster as a genetic model organism. Here, we report a statistically significant improvement in the efficiency of Drosophila cryopreservation by substituting limonene with the monoterpenoid fenchone in the embryo permeabilization step of a previously published method. We found that fenchone-permeabilized embryos exhibit greater uptake of cryoprotectant compared with those permeabilized by limonene, and an approximately sixfold increase in the rate of egg-to-adult survival for wild-type flies. Using this improved protocol, we successfully cryopreserved and revived precious strains after 12 months of storage in liquid nitrogen. These results suggest that fenchone is a superior permeabilizing agent for fly embryo cryopreservation, expanding possibilities for the long-term maintenance of Drosophila and other insect species. Further refinement of this approach may enable cryopreservation to replace continuous culture as the method of choice for routine maintenance of fly stocks.<\/jats:p>","DOI":"10.1098\/rsbl.2025.0808","type":"journal-article","created":{"date-parts":[[2026,3,18]],"date-time":"2026-03-18T00:12:21Z","timestamp":1773792741000},"source":"Crossref","is-referenced-by-count":0,"title":["Permeabilization with fenchone enhances cryopreservation of\n                    <i>Drosophila<\/i>\n                    embryos"],"prefix":"10.1098","volume":"22","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8344-7072","authenticated-orcid":false,"given":"David M.","family":"Zimmerman","sequence":"first","affiliation":[{"name":"Harvard Medical School 1 , , ,","place":["Boston, MA, USA, 02115"],"department":["Biophysics Graduate Program"]},{"name":"Harvard University 2 , , ,","place":["Cambridge, MA, USA, 02138"],"department":["Department of Physics"]},{"name":"Harvard University 3 , , ,","place":["Cambridge, MA, USA, 02138"],"department":["Center for Brain Science"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0005-9428-2110","authenticated-orcid":false,"given":"Rachel H.","family":"Yin","sequence":"additional","affiliation":[{"name":"Harvard University 2 , , ,","place":["Cambridge, MA, USA, 02138"],"department":["Department of Physics"]},{"name":"Harvard University 3 , , ,","place":["Cambridge, MA, USA, 02138"],"department":["Center for Brain Science"]},{"name":"University of Washington 4 , , ,","place":["Seattle, WA, USA, 98195"],"department":["Department of Bioengineering"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0009-3156-9359","authenticated-orcid":false,"given":"Madyson","family":"Vaca","sequence":"additional","affiliation":[{"name":"Harvard University 3 , , ,","place":["Cambridge, MA, USA, 02138"],"department":["Center for Brain Science"]},{"name":"La Sierra University 5 , , ,","place":["Riverside, CA, USA, 92505"],"department":["Department of Biology"]},{"name":"Harvard University 6 , , ,","place":["Cambridge, MA, USA, 02138"],"department":["Department of Organismic and Evolutionary Biology"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1672-8720","authenticated-orcid":false,"given":"Aravinthan D. 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