{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,12]],"date-time":"2026-05-12T22:11:23Z","timestamp":1778623883902,"version":"3.51.4"},"reference-count":35,"publisher":"The Royal Society","issue":"64","license":[{"start":{"date-parts":[[2011,4,28]],"date-time":"2011-04-28T00:00:00Z","timestamp":1303948800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/royalsociety.org\/journals\/ethics-policies\/data-sharing-mining\/"}],"content-domain":{"domain":["royalsocietypublishing.org"],"crossmark-restriction":true},"short-container-title":["J. R. Soc. Interface."],"published-print":{"date-parts":[[2011,11,7]]},"abstract":"<jats:p>\n                    Internal pH regulation is vital for many cell functions, including transport mechanisms and metabolic enzyme activity. More specifically, transport mechanisms are to a wide degree governed by internal pH distributions. We introduce the term standard deviation of the intracellular pH (s.d.(pH\n                    <jats:sub>int<\/jats:sub>\n                    )) to describe the internal pH distributions. The cellular pH distributional response to external stress such as heat has not previously been determined. In this study, the intracellular pH (pH\n                    <jats:sub>i<\/jats:sub>\n                    ) and the s.d.(pH\n                    <jats:sub>int<\/jats:sub>\n                    ) of\n                    <jats:italic>Saccharomyces cerevisiae<\/jats:italic>\n                    cells exposed to supralethal temperatures were measured using fluorescence ratio imaging microscopy (FRIM). An exponential decline in pH\n                    <jats:sub>i<\/jats:sub>\n                    was observed after an initial small decline. For the first time, we report the use of FRIM for determining\n                    <jats:italic>in vivo<\/jats:italic>\n                    plasma membrane proton permeability coefficients in yeast. Furthermore, the exponential decay of pH\n                    <jats:sub>i<\/jats:sub>\n                    and the rupture of the cell plasma membrane, as measured by propidium iodide staining, at 70\u00b0C were not simultaneous but were separated by a significant temporal difference. Finally, a nonlinear relationship between the pH\n                    <jats:sub>i<\/jats:sub>\n                    and s.d.(pH\n                    <jats:sub>int<\/jats:sub>\n                    ) was found; i.e. the s.d.(pH\n                    <jats:sub>int<\/jats:sub>\n                    ) was significantly more sensitive to supralethal temperatures than pH\n                    <jats:sub>i<\/jats:sub>\n                    . s.d.(pH\n                    <jats:sub>int<\/jats:sub>\n                    ) is therefore proposed as an early health\/vitality indicator in\n                    <jats:italic>S. cerevisiae<\/jats:italic>\n                    cells exposed to heat stress.\n                  <\/jats:p>","DOI":"10.1098\/rsif.2011.0148","type":"journal-article","created":{"date-parts":[[2011,4,29]],"date-time":"2011-04-29T05:12:54Z","timestamp":1304053974000},"page":"1635-1643","update-policy":"https:\/\/doi.org\/10.1098\/crossmark-policy","source":"Crossref","is-referenced-by-count":11,"title":["Intracellular pH distribution as a cell health indicator in\n                    <i>Saccharomyces cerevisiae<\/i>"],"prefix":"10.1098","volume":"8","author":[{"given":"Thomas","family":"Aabo","sequence":"first","affiliation":[{"name":"Department of Food Science, University of Copenhagen, Frederiksberg, Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jesper","family":"Gl\u00fcckstad","sequence":"additional","affiliation":[{"name":"Department of Photonic Engineering, DTU Fotonik, Technical University of Denmark, Kongens Lyngby, Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Henrik","family":"Siegumfeldt","sequence":"additional","affiliation":[{"name":"Department of Food Science, University of Copenhagen, Frederiksberg, Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nils","family":"Arneborg","sequence":"additional","affiliation":[{"name":"Department of Food Science, University of Copenhagen, Frederiksberg, Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"175","published-online":{"date-parts":[[2011,4,28]]},"reference":[{"key":"e_1_3_1_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.enzmictec.2008.09.005"},{"key":"e_1_3_1_3_2","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.104.4.1019"},{"key":"e_1_3_1_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1074-5521(99)80059-3"},{"key":"e_1_3_1_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijfoodmicro.2010.01.026"},{"key":"e_1_3_1_6_2","doi-asserted-by":"crossref","first-page":"1110","DOI":"10.1128\/AEM.65.3.1110-1116.1999","article-title":"Physiological characterization of viable-but-nonculturable Campylobacter jejuni cells","volume":"65","author":"Tholozan J. 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