{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T11:55:53Z","timestamp":1790769353168,"version":"4.1.0"},"reference-count":76,"publisher":"The Royal Society","issue":"118","license":[{"start":{"date-parts":[[2016,5,1]],"date-time":"2016-05-01T00:00:00Z","timestamp":1462060800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/royalsociety.org\/journals\/ethics-policies\/data-sharing-mining\/"}],"funder":[{"name":"Institute of Life Sciences, University of Southampton"},{"DOI":"10.13039\/501100000781","name":"European Research Council","doi-asserted-by":"publisher","award":["638258"],"award-info":[{"award-number":["638258"]}],"id":[{"id":"10.13039\/501100000781","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000268","name":"Biotechnology and Biological Sciences Research Council","doi-asserted-by":"publisher","award":["BB\/L021072\/1"],"award-info":[{"award-number":["BB\/L021072\/1"]}],"id":[{"id":"10.13039\/501100000268","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["royalsocietypublishing.org"],"crossmark-restriction":true},"short-container-title":["J. R. Soc. Interface."],"published-print":{"date-parts":[[2016,5]]},"abstract":"<jats:p>The use of skeletal stem cells (SSCs) for cell-based therapies is currently one of the most promising areas for skeletal disease treatment and skeletal tissue repair. The ability for controlled modification of SSCs could provide significant therapeutic potential in regenerative medicine, with the prospect to permanently repopulate a host with stem cells and their progeny. Currently, SSC differentiation into the stromal lineages of bone, fat and cartilage is assessed using different approaches that typically require cell fixation or lysis, which are invasive or even destructive. Raman spectroscopy and coherent anti-Stokes Raman scattering (CARS) microscopy present an exciting alternative for studying biological systems in their natural state, without any perturbation. Here we review the applications of Raman spectroscopy and CARS imaging in stem-cell research, and discuss the potential of these two techniques for evaluating SSCs, skeletal tissues and skeletal regeneration as an exemplar.<\/jats:p>","DOI":"10.1098\/rsif.2016.0182","type":"journal-article","created":{"date-parts":[[2016,5,11]],"date-time":"2016-05-11T09:13:01Z","timestamp":1462957981000},"page":"20160182","update-policy":"https:\/\/doi.org\/10.1098\/crossmark-policy","source":"Crossref","is-referenced-by-count":60,"title":["Raman spectroscopy and coherent anti-Stokes Raman scattering imaging: prospective tools for monitoring skeletal cells and skeletal regeneration"],"prefix":"10.1098","volume":"13","author":[{"given":"Catarina Costa","family":"Moura","sequence":"first","affiliation":[{"name":"Department of Chemistry and Institute for Life Sciences, University of Southampton, Highfield Campus, Southampton SO17 1BJ, UK"},{"name":"Bone and Joint Research Group, Centre for Human Development, Stem Cells and Regeneration, Institute of Developmental Sciences, University of Southampton, Southampton SO16 6YD, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rahul S.","family":"Tare","sequence":"additional","affiliation":[{"name":"Bone and Joint Research Group, Centre for Human Development, Stem Cells and Regeneration, Institute of Developmental Sciences, University of Southampton, Southampton SO16 6YD, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Richard O. C.","family":"Oreffo","sequence":"additional","affiliation":[{"name":"Bone and Joint Research Group, Centre for Human Development, Stem Cells and Regeneration, Institute of Developmental Sciences, University of Southampton, Southampton SO16 6YD, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sumeet","family":"Mahajan","sequence":"additional","affiliation":[{"name":"Department of Chemistry and Institute for Life Sciences, University of Southampton, Highfield Campus, Southampton SO17 1BJ, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"175","published-online":{"date-parts":[[2016,5]]},"reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.2492\/inflammregen.32.072"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1111\/jcmm.12532"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1002\/stem.1559"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1038\/35102150"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1243\/09544119JEIM750"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.2217\/rme.14.48"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature04961"},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature07041"},{"key":"e_1_3_3_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/B978-0-7020-3147-2.00002-X"},{"key":"e_1_3_3_11_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-cellbio-100913-013132"},{"key":"e_1_3_3_12_2","doi-asserted-by":"publisher","DOI":"10.1634\/stemcells.19-3-180"},{"key":"e_1_3_3_13_2","doi-asserted-by":"publisher","DOI":"10.1242\/dev.102210"},{"key":"e_1_3_3_14_2","doi-asserted-by":"publisher","DOI":"10.1016\/B978-012436643-5\/50129-2"},{"key":"e_1_3_3_15_2","doi-asserted-by":"publisher","DOI":"10.1002\/term.1577"},{"key":"e_1_3_3_16_2","doi-asserted-by":"publisher","DOI":"10.1634\/stemcells.2007-0197"},{"key":"e_1_3_3_17_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00018-013-1462-6"},{"key":"e_1_3_3_18_2","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-60761-999-4_17"},{"key":"e_1_3_3_19_2","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-60761-999-4_19"},{"key":"e_1_3_3_20_2","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-60761-999-4_20"},{"key":"e_1_3_3_21_2","doi-asserted-by":"publisher","DOI":"10.1002\/jcb.20136"},{"key":"e_1_3_3_22_2","doi-asserted-by":"publisher","DOI":"10.1002\/jrs.2975"},{"key":"e_1_3_3_23_2","doi-asserted-by":"publisher","DOI":"10.1089\/ten.teb.2011.0199"},{"key":"e_1_3_3_24_2","doi-asserted-by":"publisher","DOI":"10.1088\/2040-8978\/15\/9\/094006"},{"key":"e_1_3_3_25_2","doi-asserted-by":"publisher","DOI":"10.1002\/jbio.201300110"},{"key":"e_1_3_3_26_2","doi-asserted-by":"publisher","DOI":"10.1089\/ten.tec.2013.0472"},{"key":"e_1_3_3_27_2","doi-asserted-by":"publisher","DOI":"10.1039\/c0an00525h"},{"key":"e_1_3_3_28_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1165758"},{"key":"e_1_3_3_29_2","doi-asserted-by":"crossref","first-page":"941","DOI":"10.1002\/cphy.c120025","article-title":"Label-free analysis of cellular biochemistry by Raman spectroscopy and microscopy","volume":"3","author":"Schie IW","year":"2013","journal-title":"Compr. Physiol"},{"key":"e_1_3_3_30_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.tibtech.2014.03.002"},{"key":"e_1_3_3_31_2","doi-asserted-by":"publisher","DOI":"10.1039\/C4AN00155A"},{"key":"e_1_3_3_32_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.copbio.2009.02.006"},{"key":"e_1_3_3_33_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.gde.2011.09.003"},{"key":"e_1_3_3_34_2","doi-asserted-by":"publisher","DOI":"10.1002\/cyto.a.20299"},{"key":"e_1_3_3_35_2","doi-asserted-by":"publisher","DOI":"10.1002\/jbio.200910041"},{"key":"e_1_3_3_36_2","doi-asserted-by":"publisher","DOI":"10.1002\/jrs.2161"},{"key":"e_1_3_3_37_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0065438"},{"key":"e_1_3_3_38_2","doi-asserted-by":"publisher","DOI":"10.1021\/ac0498720"},{"key":"e_1_3_3_39_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0084478"},{"key":"e_1_3_3_40_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.molstruc.2004.12.046"},{"key":"e_1_3_3_41_2","doi-asserted-by":"publisher","DOI":"10.1021\/ac801665m"},{"key":"e_1_3_3_42_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbagen.2013.01.030"},{"key":"e_1_3_3_43_2","doi-asserted-by":"publisher","DOI":"10.1021\/ac902697q"},{"key":"e_1_3_3_44_2","doi-asserted-by":"publisher","DOI":"10.1039\/c2an35507h"},{"key":"e_1_3_3_45_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.stemcr.2015.05.005"},{"key":"e_1_3_3_46_2","doi-asserted-by":"publisher","DOI":"10.1039\/c1an15167c"},{"key":"e_1_3_3_47_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.actbio.2015.04.017"},{"key":"e_1_3_3_48_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.vibspec.2004.01.014"},{"key":"e_1_3_3_49_2","doi-asserted-by":"publisher","DOI":"10.1002\/cyto.a.22777"},{"key":"e_1_3_3_50_2","doi-asserted-by":"publisher","DOI":"10.1002\/jrs.2249"},{"key":"e_1_3_3_51_2","doi-asserted-by":"publisher","DOI":"10.1039\/c2an36579k"},{"key":"e_1_3_3_52_2","doi-asserted-by":"publisher","DOI":"10.1038\/nphoton.2015.60"},{"key":"e_1_3_3_53_2","doi-asserted-by":"publisher","DOI":"10.1038\/nchembio.525"},{"key":"e_1_3_3_54_2","doi-asserted-by":"publisher","DOI":"10.1366\/000370277774463625"},{"key":"e_1_3_3_55_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.anchem.1.031207.112754"},{"key":"e_1_3_3_56_2","doi-asserted-by":"crossref","first-page":"3","DOI":"10.1007\/978-90-481-9977-8_1","volume-title":"Biophotonics: spectroscopy, imaging, sensing, and manipulation","author":"Ohulchanskyy TY","year":"2011"},{"key":"e_1_3_3_57_2","doi-asserted-by":"publisher","DOI":"10.1021\/ac070544k"},{"key":"e_1_3_3_58_2","doi-asserted-by":"publisher","DOI":"10.1039\/b822354h"},{"key":"e_1_3_3_59_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0508282102"},{"key":"e_1_3_3_60_2","doi-asserted-by":"publisher","DOI":"10.1002\/jbio.201200104"},{"key":"e_1_3_3_61_2","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.27672"},{"key":"e_1_3_3_62_2","doi-asserted-by":"publisher","DOI":"10.1364\/OE.16.002168"},{"key":"e_1_3_3_63_2","doi-asserted-by":"publisher","DOI":"10.1007\/s11745-011-3525-5"},{"key":"e_1_3_3_64_2","doi-asserted-by":"publisher","DOI":"10.1117\/1.JBO.17.11.116011"},{"key":"e_1_3_3_65_2","doi-asserted-by":"publisher","DOI":"10.1002\/jrs.3171"},{"key":"e_1_3_3_66_2","doi-asserted-by":"publisher","DOI":"10.1364\/BOE.5.001378"},{"key":"e_1_3_3_67_2","doi-asserted-by":"publisher","DOI":"10.1039\/C5SC02168E"},{"key":"e_1_3_3_68_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.addr.2015.03.009"},{"key":"e_1_3_3_69_2","doi-asserted-by":"publisher","DOI":"10.1002\/jbio.201200138"},{"key":"e_1_3_3_70_2","doi-asserted-by":"publisher","DOI":"10.1126\/scitranslmed.aaa2384"},{"key":"e_1_3_3_71_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00216-015-9021-7"},{"key":"e_1_3_3_72_2","doi-asserted-by":"publisher","DOI":"10.1038\/bonekey.2014.97"},{"key":"e_1_3_3_73_2","doi-asserted-by":"publisher","DOI":"10.1021\/ar400331q"},{"key":"e_1_3_3_74_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.aaa8870"},{"key":"e_1_3_3_75_2","doi-asserted-by":"publisher","DOI":"10.1038\/nphoton.2012.263"},{"key":"e_1_3_3_76_2","unstructured":"Leica Microsystems. 2016 CARS microscope: label free imaging Leica TCS SP8 CARS. See http:\/\/www.leica-microsystems.com\/products\/confocal-microscopes\/details\/product\/leica-tcs-sp8-cars\/."},{"key":"e_1_3_3_77_2","doi-asserted-by":"publisher","DOI":"10.1002\/lpor.201200049"}],"container-title":["Journal of The Royal Society Interface"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/royalsocietypublishing.org\/doi\/pdf\/10.1098\/rsif.2016.0182","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/royalsocietypublishing.org\/doi\/full-xml\/10.1098\/rsif.2016.0182","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/royalsocietypublishing.org\/doi\/pdf\/10.1098\/rsif.2016.0182","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,6,21]],"date-time":"2022-06-21T13:19:22Z","timestamp":1655817562000},"score":1,"resource":{"primary":{"URL":"https:\/\/royalsocietypublishing.org\/doi\/10.1098\/rsif.2016.0182"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2016,5]]},"references-count":76,"journal-issue":{"issue":"118","published-print":{"date-parts":[[2016,5]]}},"alternative-id":["10.1098\/rsif.2016.0182"],"URL":"https:\/\/doi.org\/10.1098\/rsif.2016.0182","relation":{},"ISSN":["1742-5689","1742-5662"],"issn-type":[{"value":"1742-5689","type":"print"},{"value":"1742-5662","type":"electronic"}],"subject":[],"published":{"date-parts":[[2016,5]]},"assertion":[{"value":"2016-03-04","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2016-04-13","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2016-05-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}