{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,19]],"date-time":"2025-11-19T00:07:48Z","timestamp":1763510868376,"version":"3.45.0"},"reference-count":53,"publisher":"The Royal Society","issue":"232","license":[{"start":{"date-parts":[[2025,11,1]],"date-time":"2025-11-01T00:00:00Z","timestamp":1761955200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/royalsociety.org\/-\/media\/journals\/author\/Licence-to-Publish-20062019-final.pdf"},{"start":{"date-parts":[[2025,11,1]],"date-time":"2025-11-01T00:00:00Z","timestamp":1761955200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/royalsociety.org\/journals\/ethics-policies\/data-sharing-mining\/"}],"funder":[{"name":"Vinzenz Group"}],"content-domain":{"domain":["royalsocietypublishing.org"],"crossmark-restriction":true},"short-container-title":["J. R. Soc. Interface."],"published-print":{"date-parts":[[2025,11]]},"abstract":"<jats:p>\n                    The spatial organization of organic\/inorganic components at the cartilage\u2013bone interface in the human growth plate remains poorly understood. This study uses time-of-flight secondary ion mass spectrometry (ToF-SIMS) for the first time on human growth plate samples to provide insights into these interfaces. Three polydactyly specimens were analysed to map spatial variations of hydroxyapatite and collagen-related components. Regions of interest were statistically evaluated using Dunn\u2019s multiple comparisons and Spearman correlations. The calcified cartilage interface represents a critical transition zone, marked by a sharp decline in organic matrix components (e.g. CH\u2084N\u207a) and a concurrent increase in mineral fragments (CaPO\u2082\u207a, Ca\u207a), particularly evident in large-area images. A significant peak in the Ca\u207a\/CH\u2084N\u207a ratio (\n                    <jats:italic toggle=\"yes\">p<\/jats:italic>\n                    &lt; 0.0001) highlighted a rapid shift from organic-rich to mineral-rich composition. Correlations between organic (CNO\u207b) and mineral (PO\u2082\u207b) markers indicated early-stage mineralization, while phosphate and mineralized bone fragments confirmed full mineralization (trabecular and cortical bone). Results were consistent with existing literature, supporting a structured, progressive mineralization pattern characteristic of endochondral ossification. This study demonstrates the first application of ToF-SIMS on human growth plate samples from polydactyly cases, highlighting the capacity of ToF-SIMS for large-scale imaging of human growth plate interfaces and its potential relevance to orthopaedic research.\n                  <\/jats:p>","DOI":"10.1098\/rsif.2025.0288","type":"journal-article","created":{"date-parts":[[2025,11,19]],"date-time":"2025-11-19T00:05:12Z","timestamp":1763510712000},"update-policy":"https:\/\/doi.org\/10.1098\/crossmark-policy","source":"Crossref","is-referenced-by-count":0,"title":["Large-area time-of-flight secondary ion mass spectrometry (ToF-SIMS) imaging of the human growth plate from polydactyly specimen"],"prefix":"10.1098","volume":"22","author":[{"ORCID":"https:\/\/orcid.org\/0009-0009-0403-4180","authenticated-orcid":true,"given":"Ruth","family":"Zoehrer","sequence":"first","affiliation":[{"name":"Michael Ogon Laboratory for Orthopaedic Research, Orthopaedic Hospital Speising","place":["Vienna, Austria"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Florian","family":"Fahrnberger","sequence":"additional","affiliation":[{"name":"Institute of Chemical Technologies and Analytics, Technical University Vienna","place":["Vienna, Austria"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Herbert","family":"Hutter","sequence":"additional","affiliation":[{"name":"Institute of Chemical Technologies and Analytics, Technical University Vienna","place":["Vienna, Austria"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6046-0365","authenticated-orcid":true,"given":"Markus A.","family":"Hartmann","sequence":"additional","affiliation":[{"name":"Ludwig Boltzmann Institute of Osteology at Hanusch Hospital of OEGK and AUVA Trauma Centre Meidling, 1st Med. Dept. Hanusch Hospital","place":["Vienna, Austria"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6575-8443","authenticated-orcid":true,"given":"St\u00e9phane","family":"Blouin","sequence":"additional","affiliation":[{"name":"Ludwig Boltzmann Institute of Osteology at Hanusch Hospital of OEGK and AUVA Trauma Centre Meidling, 1st Med. Dept. Hanusch Hospital","place":["Vienna, Austria"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sebastian","family":"Farr","sequence":"additional","affiliation":[{"name":"Department of Pediatric Orthopedics, Orthopaedic Hospital Speising","place":["Vienna, Austria"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jochen G.","family":"Hofstaetter","sequence":"additional","affiliation":[{"name":"Michael Ogon Laboratory for Orthopaedic Research, Orthopaedic Hospital Speising","place":["Vienna, Austria"]},{"name":"2nd Department, Orthopaedic Hospital Speising","place":["Vienna, Austria"]}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"175","published-online":{"date-parts":[[2025,11,19]]},"reference":[{"key":"e_1_3_8_2_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature01657"},{"key":"e_1_3_8_3_2","doi-asserted-by":"publisher","DOI":"10.1007\/s11914-023-00822-6"},{"key":"e_1_3_8_4_2","doi-asserted-by":"publisher","DOI":"10.22203\/eCM.v015a05"},{"key":"e_1_3_8_5_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00198-005-2035-9"},{"key":"e_1_3_8_6_2","doi-asserted-by":"publisher","DOI":"10.1038\/nmat1545"},{"key":"e_1_3_8_7_2","doi-asserted-by":"publisher","DOI":"10.1002\/advs.202002524"},{"key":"e_1_3_8_8_2","first-page":"14","article-title":"Bone development","volume":"80","author":"Rolian C","year":"2020","unstructured":"Rolian C, Berendsen AD, Olsen BR. 2020 Bone development. Wiley Interdiscip. Rev. Dev. Biol. 80, 14\u201318. (doi:10.1016\/j.bone.2015.04.035)","journal-title":"Wiley Interdiscip. Rev. Dev. Biol."},{"key":"e_1_3_8_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.bonr.2021.101088"},{"key":"e_1_3_8_10_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00438-022-01930-1"},{"key":"e_1_3_8_11_2","doi-asserted-by":"publisher","DOI":"10.3389\/fchem.2023.1237408"},{"key":"e_1_3_8_12_2","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.3.6.1011"},{"key":"e_1_3_8_13_2","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.2.4.253"},{"key":"e_1_3_8_14_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1749-6632.1955.tb40054.x"},{"key":"e_1_3_8_15_2","doi-asserted-by":"publisher","DOI":"10.1016\/0022-2836(91)90752-R"},{"key":"e_1_3_8_16_2","doi-asserted-by":"publisher","DOI":"10.3389\/fgene.2021.726474"},{"key":"e_1_3_8_17_2","doi-asserted-by":"publisher","DOI":"10.1055\/s-0043-1777096"},{"key":"e_1_3_8_18_2","doi-asserted-by":"publisher","DOI":"10.3109\/03008209609029214"},{"key":"e_1_3_8_19_2","doi-asserted-by":"publisher","DOI":"10.1302\/0301-620X.103B2.BJJ-2020-1341.R2"},{"key":"e_1_3_8_20_2","doi-asserted-by":"publisher","DOI":"10.2106\/JBJS.RVW.21.00147"},{"key":"e_1_3_8_21_2","doi-asserted-by":"publisher","DOI":"10.1002\/anie.199410231"},{"key":"e_1_3_8_22_2","doi-asserted-by":"publisher","DOI":"10.1116\/6.0000051"},{"key":"e_1_3_8_23_2","article-title":"Secondary ion mass spectrometry for bone research","volume":"11","author":"Kern C","year":"2023","unstructured":"Kern C, Kern S, Henss A, Rohnke M. 2023 Secondary ion mass spectrometry for bone research. Biointerphases 11, 02A302. (doi:10.1116\/6.0002820)","journal-title":"Biointerphases"},{"key":"e_1_3_8_24_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jsbmb.2019.02.015"},{"key":"e_1_3_8_25_2","doi-asserted-by":"publisher","DOI":"10.1017\/S1431927621013593"},{"key":"e_1_3_8_26_2","doi-asserted-by":"publisher","DOI":"10.1007\/978-3-642-82256-8_125"},{"key":"e_1_3_8_27_2","doi-asserted-by":"publisher","DOI":"10.1007\/s40279-013-0100-7"},{"key":"e_1_3_8_28_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.actbio.2012.06.014"},{"key":"e_1_3_8_29_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.vibspec.2005.02.015"},{"key":"e_1_3_8_30_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00223-015-9971-y"},{"key":"e_1_3_8_31_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00198-011-1688-9"},{"key":"e_1_3_8_32_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00216-010-4155-0"},{"key":"e_1_3_8_33_2","doi-asserted-by":"publisher","DOI":"10.1039\/B402005G"},{"key":"e_1_3_8_34_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1047-8477(02)00635-4"},{"key":"e_1_3_8_35_2","doi-asserted-by":"publisher","DOI":"10.1016\/S8756-3282(02)00973-0"},{"key":"e_1_3_8_36_2","doi-asserted-by":"publisher","DOI":"10.1116\/1.4928211"},{"key":"e_1_3_8_37_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.apsusc.2013.10.177"},{"key":"e_1_3_8_38_2","doi-asserted-by":"publisher","DOI":"10.1039\/c3ja50059d"},{"key":"e_1_3_8_39_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00223-021-00832-5"},{"key":"e_1_3_8_40_2","doi-asserted-by":"publisher","DOI":"10.1002\/sia.1438"},{"key":"e_1_3_8_41_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.apsusc.2004.03.100"},{"key":"e_1_3_8_42_2","doi-asserted-by":"publisher","DOI":"10.1002\/pmic.200800198"},{"key":"e_1_3_8_43_2","doi-asserted-by":"publisher","DOI":"10.1097\/00003086-198403000-00033"},{"key":"e_1_3_8_44_2","doi-asserted-by":"publisher","DOI":"10.1098\/rsif.2013.0332"},{"key":"e_1_3_8_45_2","doi-asserted-by":"publisher","DOI":"10.1038\/bonekey.2013.181"},{"key":"e_1_3_8_46_2","doi-asserted-by":"publisher","DOI":"10.1117\/1.429916"},{"key":"e_1_3_8_47_2","doi-asserted-by":"publisher","DOI":"10.1111\/nyas.13572"},{"key":"e_1_3_8_48_2","doi-asserted-by":"publisher","DOI":"10.1359\/jbmr.1999.14.11.1916"},{"key":"e_1_3_8_49_2","doi-asserted-by":"publisher","DOI":"10.1089\/ten.1999.5.25"},{"key":"e_1_3_8_50_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.bone.2022.116580"},{"key":"e_1_3_8_51_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.bone.2022.116548"},{"key":"e_1_3_8_52_2","doi-asserted-by":"publisher","DOI":"10.3390\/ijms222413329"},{"key":"e_1_3_8_53_2","doi-asserted-by":"publisher","DOI":"10.3390\/cells12212576"},{"key":"e_1_3_8_54_2","unstructured":"Zoehrer R Fahrnberger F Hutter H Hartmann MA Blouin S Farr S et al. 2025 Supplementary material from: Large-area Time-of-Flight Secondary Ion Mass Spectrometry (TOF-SIMS) Imaging of the Human Growth Plate from Polydactyly Specimen. Figshare. (doi:10.6084\/m9.figshare.c.8085128)"}],"container-title":["Journal of The Royal Society Interface"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/royalsocietypublishing.org\/doi\/pdf\/10.1098\/rsif.2025.0288","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/royalsocietypublishing.org\/doi\/full-xml\/10.1098\/rsif.2025.0288","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/royalsocietypublishing.org\/doi\/pdf\/10.1098\/rsif.2025.0288","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,11,19]],"date-time":"2025-11-19T00:05:16Z","timestamp":1763510716000},"score":1,"resource":{"primary":{"URL":"https:\/\/royalsocietypublishing.org\/doi\/10.1098\/rsif.2025.0288"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,11]]},"references-count":53,"journal-issue":{"issue":"232","published-print":{"date-parts":[[2025,11]]}},"alternative-id":["10.1098\/rsif.2025.0288"],"URL":"https:\/\/doi.org\/10.1098\/rsif.2025.0288","relation":{},"ISSN":["1742-5662"],"issn-type":[{"value":"1742-5662","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,11]]},"assertion":[{"value":"2025-04-14","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-07-30","order":1,"name":"revised","label":"Revised","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-09-29","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-11-19","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"20250288"}}