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R. Soc. Interface."],"published-print":{"date-parts":[[2025,10]]},"abstract":"<jats:p>\n            Epidemiological models of mosquito-borne virus transmission often lack accurate estimates of host-to-vector transmission probability. Here, we estimated this probability for two strains of Zika virus (ZIKV)\u2014one sylvatic and one human-endemic\u2014from two monkey species to\n            <jats:italic toggle=\"yes\">Aedes albopictus<\/jats:italic>\n            mosquitoes using experimental infection data. Viral dynamics did not differ between monkey species, although one (cynomolgus macaque) is a native ZIKV host and the other (squirrel monkey) a novel host, but did differ between strains, with viremia for the human-endemic strain peaking later and lower than the sylvatic strain. Only the sylvatic strain was transmitted to mosquitoes. Within mosquitoes, anatomical barriers influence viral progression to salivary glands, complicating host infectiousness estimation. We quantified the probability of viral dissemination to the legs in\n            <jats:italic toggle=\"yes\">Ae. albopictus<\/jats:italic>\n            , which increased with host viral load and was higher after feeding on squirrel monkeys than on cynomolgus macaques. We also found a positive relationship between virus titre in mosquito legs and virus detection in saliva after a 14-day extrinsic incubation period. Combining these factors, we found that squirrel monkeys were on average 1.5 times more infectious to\n            <jats:italic toggle=\"yes\">Ae. albopictus<\/jats:italic>\n            than cynomolgus macaques. These estimates will help assess ZIKV\u2019s potential to establish an enzootic, sylvatic cycle in the Americas.\n          <\/jats:p>","DOI":"10.1098\/rsif.2025.0365","type":"journal-article","created":{"date-parts":[[2025,9,30]],"date-time":"2025-09-30T23:06:40Z","timestamp":1759273600000},"update-policy":"https:\/\/doi.org\/10.1098\/crossmark-policy","source":"Crossref","is-referenced-by-count":0,"title":["Inside-out: modelling the link between Zika virus viral dynamics within hosts and transmission to vectors across host species and virus strains"],"prefix":"10.1098","volume":"22","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5687-3570","authenticated-orcid":true,"given":"H\u00e9l\u00e8ne","family":"Cecilia","sequence":"first","affiliation":[{"name":"Department of Biology, New Mexico State University, Las Cruces, NM 88003, USA"},{"name":"Oniris, INRAE, BIOEPAR","place":["44300 Nantes, France"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Benjamin 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