{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,26]],"date-time":"2026-09-26T02:59:10Z","timestamp":1790391550049,"version":"4.1.0"},"reference-count":59,"publisher":"The Royal Society","issue":"238","license":[{"start":{"date-parts":[[2026,5,20]],"date-time":"2026-05-20T00:00:00Z","timestamp":1779235200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,5,20]],"date-time":"2026-05-20T00:00:00Z","timestamp":1779235200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Department of Research and Universities of the Generalitat de Catalunya"},{"DOI":"10.13039\/501100004837","name":"Spanish Ministry of Science and Innovation","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100004837","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Networking Biomedical Research Center (CIBER) of Spain"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,5,20]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Cells tightly regulate membrane composition to maintain homeostasis and adapt to perturbations. Disruptions in lipid metabolism, such as those occurring in Gaucher disease (GD), can disturb this balance. In GD, excess glucosylceramide (GlcCer) accumulates in lysosomes, altering overall lipid metabolism and potentially impacting membrane composition. This study investigates how GlcCer influences lipid distribution and the local mechanical properties of model lipid bilayers composed of 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC), egg sphingomyelin (SME), cholesterol (Chol) and GlcCer in varying ratios, using atomic force microscopy and force spectroscopy. In simple bilayers (DOPC and DOPC:Chol), GlcCer forms rigid, unstable domains with high lateral packing. In contrast, in complex DOPC:Chol:SME membranes, higher GlcCer concentrations are accommodated but significantly alter domain organization and nanomechanical properties. Excess GlcCer preferentially partitions into segregated domains, sequestering cholesterol and SME, thereby reshaping lipid distribution with potential implications for membrane curvature, protein diffusion and localization.<\/jats:p>","DOI":"10.1098\/rsif.2025.1168","type":"journal-article","created":{"date-parts":[[2026,5,20]],"date-time":"2026-05-20T00:09:17Z","timestamp":1779235757000},"source":"Crossref","is-referenced-by-count":1,"title":["Glucosylceramide increases the mechanical resistance of membrane domains"],"prefix":"10.1098","volume":"23","author":[{"given":"Marina","family":"Placci","sequence":"first","affiliation":[{"name":"Institute for Bioengineering of Catalonia (IBEC), The Barcelona Institute of Science and Technology (BIST) 1 , ,","place":["Barcelona, Spain"],"department":["Nanoprobes and Nanoswitches Group"]},{"name":"Institute for Bioengineering of Catalonia (IBEC), The Barcelona Institute of Science and Technology (BIST) 2 , ,","place":["Barcelona, Spain"],"department":["Targeted Therapeutics and Nanodevices Group"]},{"name":"University of Barcelona (UB) 3 , ,","place":["Barcelona, Spain"],"department":["Doctoral Program in Biotechnology"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Silvia","family":"Muro","sequence":"additional","affiliation":[{"name":"Institute for Bioengineering of Catalonia (IBEC), The Barcelona Institute of Science and Technology (BIST) 2 , ,","place":["Barcelona, Spain"],"department":["Targeted Therapeutics and Nanodevices Group"]},{"name":"Institute of Catalonia for Research and Advanced Studies (ICREA) 4 , ,","place":["Barcelona, Spain"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0815-742X","authenticated-orcid":false,"given":"Marina In\u00e9s","family":"Giannotti","sequence":"additional","affiliation":[{"name":"Institute for Bioengineering of Catalonia (IBEC), The Barcelona Institute of Science and Technology (BIST) 1 , ,","place":["Barcelona, Spain"],"department":["Nanoprobes and Nanoswitches Group"]},{"name":"University of Barcelona (UB) 5 , ,","place":["Barcelona, Spain"],"department":["Department of Materials Science and Physical Chemistry, and IQTC"]},{"name":"Centro de Investigacion Biomedica en Red (CIBER-BBN), ISCIII 6 , ,","place":["Barcelona, Spain"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"175","published-online":{"date-parts":[[2026,5,20]]},"reference":[{"key":"2026051920091135500_R1","doi-asserted-by":"crossref","DOI":"10.1098\/rsif.2016.0228","article-title":"Mammalian phospholipid homeostasis: evidence that membrane curvature elastic stress drives homeoviscous adaptation in vivo","volume":"13","author":"Dymond","year":"2016","journal-title":"J. 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Figshare.","author":"Placci","year":"2026"}],"container-title":["Journal of the Royal Society Interface"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/royalsocietypublishing.org\/rsif\/article-pdf\/doi\/10.1098\/rsif.2025.1168\/6138904\/rsif.2025.1168.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/royalsocietypublishing.org\/rsif\/article-pdf\/doi\/10.1098\/rsif.2025.1168\/6138904\/rsif.2025.1168.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,5,20]],"date-time":"2026-05-20T00:09:26Z","timestamp":1779235766000},"score":1,"resource":{"primary":{"URL":"https:\/\/royalsocietypublishing.org\/rsif\/article\/23\/238\/20251168\/481764\/Glucosylceramide-increases-the-mechanical"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,5,20]]},"references-count":59,"journal-issue":{"issue":"238","published-print":{"date-parts":[[2026,5,20]]}},"URL":"https:\/\/doi.org\/10.1098\/rsif.2025.1168","relation":{},"ISSN":["1742-5662"],"issn-type":[{"value":"1742-5662","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,5,20]]},"article-number":"20251168"}}