{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,23]],"date-time":"2026-10-23T01:52:02Z","timestamp":1792720322294,"version":"4.5.0"},"reference-count":21,"publisher":"The Royal Society","issue":"2","license":[{"start":{"date-parts":[[2012,2,1]],"date-time":"2012-02-01T00:00:00Z","timestamp":1328054400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/royalsociety.org\/journals\/ethics-policies\/data-sharing-mining\/"}],"content-domain":{"domain":["royalsocietypublishing.org"],"crossmark-restriction":true},"short-container-title":["Open Biol."],"published-print":{"date-parts":[[2012,2]]},"abstract":"<jats:p>Here, we present a simple modular extendable vector system for introducing the T7 RNA polymerase and tetracycline repressor genes into<jats:italic>Trypanosoma brucei<\/jats:italic>. This novel system exploits developments in our understanding of gene expression and genome organization to produce a streamlined plasmid optimized for high levels of expression of the introduced transgenes. We demonstrate the utility of this novel system in bloodstream and procyclic forms of<jats:italic>Trypanosoma brucei<\/jats:italic>, including the genome strain TREU927\/4. We validate these cell lines using a variety of inducible experiments that recapture previously published lethal and non-lethal phenotypes. We further demonstrate the utility of the single marker (SmOx) TREU927\/4 cell line for<jats:italic>in vivo<\/jats:italic>experiments in the tsetse fly and provide a set of plasmids that enable both whole-fly and salivary gland-specific inducible expression of transgenes.<\/jats:p>","DOI":"10.1098\/rsob.110037","type":"journal-article","created":{"date-parts":[[2012,2,16]],"date-time":"2012-02-16T05:51:08Z","timestamp":1329371468000},"page":"110037","update-policy":"https:\/\/doi.org\/10.1098\/crossmark-policy","source":"Crossref","is-referenced-by-count":151,"title":["A modular and optimized single marker system for generating<i>Trypanosoma brucei<\/i>cell lines expressing T7 RNA polymerase and the tetracycline repressor"],"prefix":"10.1098","volume":"2","author":[{"given":"S. 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