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Our yeast system permits multiple parasite targets to be assayed in parallel owing to the strains\u2019 expression of different fluorescent proteins. A strain expressing the human target is included in the multiplexed screen to exclude compounds that do not discriminate between host and parasite enzymes. This form of assay has the advantages of using known targets and not requiring the\n            <jats:italic>in vitro<\/jats:italic>\n            culture of parasites. We performed automated screens for inhibitors of parasite dihydrofolate reductases,\n            <jats:italic>N<\/jats:italic>\n            -myristoyltransferases and phosphoglycerate kinases, finding specific inhibitors of parasite targets. We found that our \u2018hits\u2019 have significant structural similarities to compounds with\n            <jats:italic>in vitro<\/jats:italic>\n            anti-parasitic activity, validating our screens and suggesting targets for hits identified in parasite-based assays. Finally, we demonstrate a 60 per cent success rate for our hit compounds in killing or severely inhibiting the growth of\n            <jats:italic>Trypanosoma brucei<\/jats:italic>\n            , the causative agent of African sleeping sickness.\n          <\/jats:p>","DOI":"10.1098\/rsob.120158","type":"journal-article","created":{"date-parts":[[2013,2,28]],"date-time":"2013-02-28T15:19:05Z","timestamp":1362064745000},"page":"120158","update-policy":"https:\/\/doi.org\/10.1098\/crossmark-policy","source":"Crossref","is-referenced-by-count":35,"title":["Yeast-based automated high-throughput screens to identify anti-parasitic lead compounds"],"prefix":"10.1098","volume":"3","author":[{"given":"Elizabeth","family":"Bilsland","sequence":"first","affiliation":[{"name":"Cambridge Systems Biology Centre and Department of Biochemistry, University of Cambridge, Sanger Building, 80 Tennis Court Road, Cambridge CB2 1GA, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrew","family":"Sparkes","sequence":"additional","affiliation":[{"name":"Department of Computer Science, Aberystwyth University, Aberystwyth SY23 3DB, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kevin","family":"Williams","sequence":"additional","affiliation":[{"name":"Department of Computer Science, Aberystwyth University, Aberystwyth SY23 3DB, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Harry J.","family":"Moss","sequence":"additional","affiliation":[{"name":"Cambridge Systems Biology Centre and Department of Biochemistry, University of Cambridge, Sanger Building, 80 Tennis Court Road, Cambridge CB2 1GA, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michaela","family":"de Clare","sequence":"additional","affiliation":[{"name":"Cambridge Systems Biology Centre and Department of Biochemistry, University of Cambridge, Sanger Building, 80 Tennis Court Road, Cambridge CB2 1GA, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"P\u0131nar","family":"Pir","sequence":"additional","affiliation":[{"name":"Cambridge Systems Biology Centre and Department of Biochemistry, University of Cambridge, Sanger Building, 80 Tennis Court Road, Cambridge CB2 1GA, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jem","family":"Rowland","sequence":"additional","affiliation":[{"name":"Department of Computer Science, Aberystwyth University, Aberystwyth SY23 3DB, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wayne","family":"Aubrey","sequence":"additional","affiliation":[{"name":"School of Chemical Engineering and Analytical Science, University of Manchester, Manchester M13 9PL, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ron","family":"Pateman","sequence":"additional","affiliation":[{"name":"Department of Computer Science, Aberystwyth University, Aberystwyth SY23 3DB, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mike","family":"Young","sequence":"additional","affiliation":[{"name":"Institute of Biological, Environmental and Rural Sciences, Aberystwyth University, Aberystwyth SY23 3DD, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mark","family":"Carrington","sequence":"additional","affiliation":[{"name":"Cambridge Systems Biology Centre and Department of Biochemistry, University of Cambridge, Sanger Building, 80 Tennis Court Road, Cambridge CB2 1GA, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ross D.","family":"King","sequence":"additional","affiliation":[{"name":"School of Computer Science, University of Manchester, Manchester M13 9PL, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stephen G.","family":"Oliver","sequence":"additional","affiliation":[{"name":"Cambridge Systems Biology Centre and Department of Biochemistry, University of Cambridge, Sanger Building, 80 Tennis Court Road, Cambridge CB2 1GA, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"175","published-online":{"date-parts":[[2013,2]]},"reference":[{"key":"e_1_3_1_2_2","unstructured":"World Health Organization. 2011 World malaria report: 2011 . 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