{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,21]],"date-time":"2026-09-21T16:51:44Z","timestamp":1790009504106,"version":"4.0.1"},"reference-count":37,"publisher":"The Royal Society","issue":"5","license":[{"start":{"date-parts":[[2013,5,1]],"date-time":"2013-05-01T00:00:00Z","timestamp":1367366400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/royalsociety.org\/journals\/ethics-policies\/data-sharing-mining\/"}],"content-domain":{"domain":["royalsocietypublishing.org"],"crossmark-restriction":true},"short-container-title":["Open Biol."],"published-print":{"date-parts":[[2013,5]]},"abstract":"<jats:p>\n                    Glycoconjugate-based vaccines have proved to be effective at producing long-lasting protection against numerous pathogens. Here, we describe the application of bacterial protein glycan coupling technology (PGCT) to generate a novel recombinant glycoconjugate vaccine\n                    <jats:italic>.<\/jats:italic>\n                    We demonstrate the conjugation of the\n                    <jats:italic>Francisella tularensis<\/jats:italic>\n                    O-antigen to the\n                    <jats:italic>Pseudomonas aeruginosa<\/jats:italic>\n                    carrier protein exotoxin A using the\n                    <jats:italic>Campylobacter jejuni<\/jats:italic>\n                    PglB oligosaccharyltransferase\n                    <jats:italic>.<\/jats:italic>\n                    The resultant recombinant\n                    <jats:italic>F. tularensis<\/jats:italic>\n                    glycoconjugate vaccine is expressed in\n                    <jats:italic>Escherichia coli<\/jats:italic>\n                    where yields of 3 mg l\n                    <jats:sup>\u22121<\/jats:sup>\n                    of culture were routinely produced in a single-step purification process. Vaccination of BALB\/c mice with the purified glycoconjugate boosted IgG levels and significantly increased the time to death upon subsequent challenge with\n                    <jats:italic>F. tularensis<\/jats:italic>\n                    subsp.\n                    <jats:italic>holarctica<\/jats:italic>\n                    . PGCT allows different polysaccharide and protein combinations to be produced recombinantly and could be easily applicable for the production of diverse glycoconjugate vaccines.\n                  <\/jats:p>","DOI":"10.1098\/rsob.130002","type":"journal-article","created":{"date-parts":[[2013,5,22]],"date-time":"2013-05-22T20:47:03Z","timestamp":1369255623000},"page":"130002","update-policy":"https:\/\/doi.org\/10.1098\/crossmark-policy","source":"Crossref","is-referenced-by-count":88,"title":["Exploitation of bacterial\n                    <i>N<\/i>\n                    -linked glycosylation to develop a novel recombinant glycoconjugate vaccine against\n                    <i>Francisella tularensis<\/i>"],"prefix":"10.1098","volume":"3","author":[{"given":"Jon","family":"Cuccui","sequence":"first","affiliation":[{"name":"Department of Pathogen Molecular Biology, London School of Hygiene and Tropical Medicine, Keppel Street, London WC1E 7HT, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rebecca M.","family":"Thomas","sequence":"additional","affiliation":[{"name":"Defence Science and Technology Laboratory, Porton Down, Salisbury, Wiltshire SP4 0JQ, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Madeleine G.","family":"Moule","sequence":"additional","affiliation":[{"name":"Department of Pathogen Molecular Biology, London School of Hygiene and Tropical Medicine, Keppel Street, London WC1E 7HT, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Riccardo V.","family":"D'Elia","sequence":"additional","affiliation":[{"name":"Defence Science and Technology Laboratory, Porton Down, Salisbury, Wiltshire SP4 0JQ, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thomas R.","family":"Laws","sequence":"additional","affiliation":[{"name":"Defence Science and Technology Laboratory, Porton Down, Salisbury, Wiltshire SP4 0JQ, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dominic C.","family":"Mills","sequence":"additional","affiliation":[{"name":"Department of Pathogen Molecular Biology, London School of Hygiene and Tropical Medicine, Keppel Street, London WC1E 7HT, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Diane","family":"Williamson","sequence":"additional","affiliation":[{"name":"Defence Science and Technology Laboratory, Porton Down, Salisbury, Wiltshire SP4 0JQ, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Timothy P.","family":"Atkins","sequence":"additional","affiliation":[{"name":"Defence Science and Technology Laboratory, Porton Down, Salisbury, Wiltshire SP4 0JQ, UK"},{"name":"School of Biosciences, University of Exeter, Devon, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joann L.","family":"Prior","sequence":"additional","affiliation":[{"name":"Defence Science and Technology Laboratory, Porton Down, Salisbury, Wiltshire SP4 0JQ, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Brendan W.","family":"Wren","sequence":"additional","affiliation":[{"name":"Department of Pathogen Molecular Biology, London School of Hygiene and Tropical Medicine, Keppel Street, London WC1E 7HT, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"175","published-online":{"date-parts":[[2013,5]]},"reference":[{"key":"e_1_3_1_2_2","doi-asserted-by":"publisher","DOI":"10.1097\/00006454-199909000-00004"},{"key":"e_1_3_1_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(07)60564-9"},{"key":"e_1_3_1_4_2","doi-asserted-by":"publisher","DOI":"10.1093\/ije\/dyq033"},{"key":"e_1_3_1_5_2","doi-asserted-by":"publisher","DOI":"10.1345\/aph.1Q347"},{"key":"e_1_3_1_6_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.298.5599.1790"},{"key":"e_1_3_1_7_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.1999.01415.x"},{"key":"e_1_3_1_8_2","doi-asserted-by":"publisher","DOI":"10.1038\/sj.emboj.7601087"},{"key":"e_1_3_1_9_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1134351"},{"key":"e_1_3_1_10_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0500044102"},{"key":"e_1_3_1_11_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M206114200"},{"key":"e_1_3_1_12_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0509207103"},{"key":"e_1_3_1_13_2","doi-asserted-by":"publisher","DOI":"10.1099\/jmm.0.039438-0"},{"key":"e_1_3_1_14_2","doi-asserted-by":"publisher","DOI":"10.2217\/fmb.09.10"},{"key":"e_1_3_1_15_2","doi-asserted-by":"publisher","DOI":"10.1186\/1475-2859-11-13"},{"key":"e_1_3_1_16_2","doi-asserted-by":"publisher","DOI":"10.1186\/1475-2859-9-61"},{"key":"e_1_3_1_17_2","doi-asserted-by":"publisher","DOI":"10.1001\/jama.285.21.2763"},{"key":"e_1_3_1_18_2","doi-asserted-by":"crossref","first-page":"262","DOI":"10.1128\/BR.25.3.262-267.1961","article-title":"Aerosol infection of man with Pasteurella tularensis","volume":"25","author":"McCrumb FR","year":"1961","journal-title":"Bacteriol. 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