{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,14]],"date-time":"2026-10-14T01:47:41Z","timestamp":1791942461519,"version":"4.3.4"},"reference-count":48,"publisher":"The Royal Society","issue":"3","license":[{"start":{"date-parts":[[2016,3,1]],"date-time":"2016-03-01T00:00:00Z","timestamp":1456790400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/royalsociety.org\/journals\/ethics-policies\/data-sharing-mining\/"}],"funder":[{"DOI":"10.13039\/501100002858","name":"China Postdoctoral Science Foundation","doi-asserted-by":"crossref","award":["2015M572755"],"award-info":[{"award-number":["2015M572755"]}],"id":[{"id":"10.13039\/501100002858","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100012166","name":"National Basic Research Program of China","doi-asserted-by":"crossref","award":["2015CB554202"],"award-info":[{"award-number":["2015CB554202"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Foundation of State Key Laboratory of Pathogen and Biosecurity of China","award":["SKLPBS1402"],"award-info":[{"award-number":["SKLPBS1402"]}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"crossref","award":["31430006, 31471184, and 81302608"],"award-info":[{"award-number":["31430006, 31471184, and 81302608"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["royalsocietypublishing.org"],"crossmark-restriction":true},"short-container-title":["Open Biol."],"published-print":{"date-parts":[[2016,3]]},"abstract":"<jats:p>\n            RovA is known to enhance\n            <jats:italic>Yersinia pestis<\/jats:italic>\n            virulence by directly upregulating the\n            <jats:italic>psa<\/jats:italic>\n            loci. This work presents a complex gene regulatory paradigm involving the reciprocal regulatory action of RovM and RovA on the expression of biofilm and virulence genes as well as on their own genes. RovM and RovA enhance and inhibit\n            <jats:italic>Y. pestis<\/jats:italic>\n            biofilm production, respectively, whereas RovM represses virulence in mice. RovM directly stimulates the transcription of\n            <jats:italic>hmsT<\/jats:italic>\n            ,\n            <jats:italic>hmsCDE<\/jats:italic>\n            and\n            <jats:italic>rovM<\/jats:italic>\n            , while indirectly enhancing\n            <jats:italic>hmsHFRS<\/jats:italic>\n            transcription. It also indirectly represses\n            <jats:italic>hmsP<\/jats:italic>\n            transcription. By contrast, RovA directly represses\n            <jats:italic>hmsT<\/jats:italic>\n            transcription and indirectly inhibits\n            <jats:italic>waaAE-coaD<\/jats:italic>\n            transcription, while RovM inhibits\n            <jats:italic>psaABC<\/jats:italic>\n            and\n            <jats:italic>psaEF<\/jats:italic>\n            transcription by directly repressing\n            <jats:italic>rovA<\/jats:italic>\n            transcription.\n            <jats:italic>rovM<\/jats:italic>\n            expression is significantly upregulated at 26\u00b0C (the temperature of the flea gut) relative to 37\u00b0C (the warm-blooded host temperature). We speculate that upregulation of\n            <jats:italic>rovM<\/jats:italic>\n            together with downregulation of\n            <jats:italic>rovA<\/jats:italic>\n            in the flea gut would promote\n            <jats:italic>Y. pestis<\/jats:italic>\n            biofilm formation while inhibiting virulence gene expression, leading to a more transmissible infection of this pathogen in fleas. Once the bacterium shifts to a lifestyle in the warm-blooded hosts, inhibited RovM production accompanied by recovered RovA synthesis would encourage virulence factor production and inhibit biofilm gene expression.\n          <\/jats:p>","DOI":"10.1098\/rsob.150198","type":"journal-article","created":{"date-parts":[[2016,3,16]],"date-time":"2016-03-16T09:13:07Z","timestamp":1458119587000},"page":"150198","update-policy":"https:\/\/doi.org\/10.1098\/crossmark-policy","source":"Crossref","is-referenced-by-count":30,"title":["Reciprocal regulation of\n            <i>Yersinia pestis<\/i>\n            biofilm formation and virulence by RovM and RovA"],"prefix":"10.1098","volume":"6","author":[{"given":"Lei","family":"Liu","sequence":"first","affiliation":[{"name":"State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing 100071, People's Republic of 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