{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,27]],"date-time":"2026-08-27T09:45:29Z","timestamp":1787823929139,"version":"build-2784847793"},"reference-count":112,"publisher":"The Royal Society","issue":"2","license":[{"start":{"date-parts":[[2018,2,1]],"date-time":"2018-02-01T00:00:00Z","timestamp":1517443200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/royalsociety.org\/journals\/ethics-policies\/data-sharing-mining\/"}],"funder":[{"DOI":"10.13039\/501100001711","name":"Swiss National Science Foundation","doi-asserted-by":"crossref","award":["310030B-149641"],"award-info":[{"award-number":["310030B-149641"]}],"id":[{"id":"10.13039\/501100001711","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["royalsocietypublishing.org"],"crossmark-restriction":true},"short-container-title":["Open Biol."],"published-print":{"date-parts":[[2018,2]]},"abstract":"<jats:p>\n            Deregulation of centriole duplication has been implicated in cancer and primary microcephaly. Accordingly, it is important to understand how key centriole duplication factors are regulated. E3 ubiquitin ligases have been implicated in controlling the levels of several duplication factors, including PLK4, STIL and SAS-6, but the precise mechanisms ensuring centriole homeostasis remain to be fully understood. Here, we have combined proteomics approaches with the use of MLN4924, a generic inhibitor of SCF E3 ubiquitin ligases, to monitor changes in the cellular abundance of centriole duplication factors. We identified human STIL as a novel substrate of SCF-\u03b2TrCP. The binding of \u03b2TrCP depends on a DSG motif within STIL, and serine 395 within this motif is phosphorylated\n            <jats:italic>in vivo<\/jats:italic>\n            . SCF-\u03b2TrCP-mediated degradation of STIL occurs throughout interphase and mutations in the DSG motif causes massive centrosome amplification, attesting to the physiological importance of the pathway. We also uncover a connection between this new pathway and CDK2, whose role in centriole biogenesis remains poorly understood. We show that CDK2 activity protects STIL against SCF-\u03b2TrCP-mediated degradation, indicating that CDK2 and SCF-\u03b2TrCP cooperate via STIL to control centriole biogenesis.\n          <\/jats:p>","DOI":"10.1098\/rsob.170253","type":"journal-article","created":{"date-parts":[[2018,2,14]],"date-time":"2018-02-14T10:10:14Z","timestamp":1518603014000},"update-policy":"https:\/\/doi.org\/10.1098\/crossmark-policy","source":"Crossref","is-referenced-by-count":24,"title":["The SKP1-Cullin-F-box E3 ligase \u03b2TrCP and CDK2 cooperate to control STIL abundance and centriole number"],"prefix":"10.1098","volume":"8","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0841-6225","authenticated-orcid":false,"given":"Christian","family":"Arquint","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9822-4074","authenticated-orcid":false,"given":"Fabien","family":"Cubizolles","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Agathe","family":"Morand","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alexander","family":"Schmidt","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4835-5719","authenticated-orcid":false,"given":"Erich A.","family":"Nigg","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"175","published-online":{"date-parts":[[2018,2,14]]},"reference":[{"key":"e_1_3_6_2_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1209037"},{"key":"e_1_3_6_3_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrm2100"},{"key":"e_1_3_6_4_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrm2180"},{"key":"e_1_3_6_5_2","doi-asserted-by":"publisher","DOI":"10.1038\/ncb2345"},{"key":"e_1_3_6_6_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrm.2016.162"},{"key":"e_1_3_6_7_2","doi-asserted-by":"publisher","DOI":"10.1098\/rstb.2013.0464"},{"key":"e_1_3_6_8_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrm3373"},{"key":"e_1_3_6_9_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrm4062"},{"key":"e_1_3_6_10_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrm.2017.21"},{"key":"e_1_3_6_11_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrm3085"},{"key":"e_1_3_6_12_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1124534"},{"key":"e_1_3_6_13_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.tcb.2016.08.002"},{"key":"e_1_3_6_14_2","doi-asserted-by":"publisher","DOI":"10.1098\/rstb.2013.0460"},{"key":"e_1_3_6_15_2","doi-asserted-by":"publisher","DOI":"10.1101\/cshperspect.a015800"},{"key":"e_1_3_6_16_2","article-title":"Once and only once: mechanisms of centriole duplication and their deregulation in disease","author":"Nigg EA","unstructured":"Nigg EA, Holland AJ. 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