{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,23]],"date-time":"2026-10-23T20:09:36Z","timestamp":1792786176792,"version":"4.5.0"},"reference-count":41,"publisher":"The Royal Society","issue":"2","license":[{"start":{"date-parts":[[2019,2,1]],"date-time":"2019-02-01T00:00:00Z","timestamp":1548979200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/royalsociety.org\/journals\/ethics-policies\/data-sharing-mining\/"}],"funder":[{"name":"HBiGGs"},{"name":"Forschungskommission University Freiburg","award":["HEE1079\/16"],"award-info":[{"award-number":["HEE1079\/16"]}]},{"name":"German Research Foundation (DFG) and the University of Freiburg in the funding programme Open Access Publishing"}],"content-domain":{"domain":["royalsocietypublishing.org"],"crossmark-restriction":true},"short-container-title":["Open Biol."],"published-print":{"date-parts":[[2019,2]]},"abstract":"<jats:p>Optic cup morphogenesis is an intricate process. Especially, the formation of the optic fissure is not well understood. Persisting optic fissures, termed coloboma, are frequent causes for congenital blindness. Even though the defective fusion of the fissure margins is the most acknowledged reason for coloboma, highly variable morphologies of coloboma phenotypes argue for a diverse set of underlying pathomechanisms. Here, we investigate optic fissure morphogenesis in zebrafish to identify potential morphogenetic defects resulting in coloboma. We show that the formation of the optic fissure depends on tissue flow movements, integrated into the bilateral distal epithelial flow forming the optic cup. On the temporal side, the distal flow translates into a ventral perpendicular flow, shaping the temporal fissure margin. On the nasal side, however, the distal flow is complemented by tissue derived from the optic stalk, shaping the nasal fissure margin. Notably, a distinct population of TGF\u03b2-signalling positive cells is translocated from the optic stalk into both fissure margins. Furthermore, we show that induced BMP signalling as well as Wnt-signalling inhibition result in morphogenetic defects of the optic fissure. Our data also indicate that morphogenesis is crucial for a proper positioning of pre-specified dorsal\u2013ventral optic cup domains.<\/jats:p>","DOI":"10.1098\/rsob.180179","type":"journal-article","created":{"date-parts":[[2019,2,13]],"date-time":"2019-02-13T03:55:06Z","timestamp":1550030106000},"page":"180179","update-policy":"https:\/\/doi.org\/10.1098\/crossmark-policy","source":"Crossref","is-referenced-by-count":21,"title":["Morphogenesis and axis specification occur in parallel during optic cup and optic fissure formation, differentially modulated by BMP and Wnt"],"prefix":"10.1098","volume":"9","author":[{"given":"Priska","family":"Eckert","sequence":"first","affiliation":[{"name":"Department of Molecular Embryology, Institute of Anatomy and Cell Biology, Faculty of Medicine, University of Freiburg, 79104 Freiburg, Germany"},{"name":"Faculty of Biology, University of Freiburg, Schaenzlestrasse 1, 79104 Freiburg, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Max D.","family":"Knickmeyer","sequence":"additional","affiliation":[{"name":"Department of Molecular Embryology, Institute of Anatomy and Cell Biology, Faculty of Medicine, University of Freiburg, 79104 Freiburg, Germany"},{"name":"Faculty of Biology, University of Freiburg, Schaenzlestrasse 1, 79104 Freiburg, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lucas","family":"Sch\u00fctz","sequence":"additional","affiliation":[{"name":"Centre for Organismal Studies, Heidelberg University, 69120 Heidelberg, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joachim","family":"Wittbrodt","sequence":"additional","affiliation":[{"name":"Centre for Organismal Studies, Heidelberg University, 69120 Heidelberg, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7374-8886","authenticated-orcid":true,"given":"Stephan","family":"Heermann","sequence":"additional","affiliation":[{"name":"Department of Molecular Embryology, Institute of Anatomy and Cell Biology, Faculty of Medicine, University of Freiburg, 79104 Freiburg, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"175","published-online":{"date-parts":[[2019,2,13]]},"reference":[{"key":"e_1_3_6_2_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1127144"},{"key":"e_1_3_6_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.mod.2013.05.001"},{"key":"e_1_3_6_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.devcel.2013.09.023"},{"key":"e_1_3_6_5_2","volume-title":"The vertebrate eye and its adaptive radiation","author":"Walls GL","year":"1942"},{"key":"e_1_3_6_6_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.cellbio.17.1.255"},{"key":"e_1_3_6_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0039-6257(00)00151-X"},{"key":"e_1_3_6_8_2","unstructured":"Graw J. 2003 The genetic and molecular basis of congenital eye defects. 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