{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,5]],"date-time":"2026-05-05T16:22:56Z","timestamp":1777998176186,"version":"3.51.4"},"reference-count":169,"publisher":"The Royal Society","issue":"11","license":[{"start":{"date-parts":[[2020,11,1]],"date-time":"2020-11-01T00:00:00Z","timestamp":1604188800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2020,11,1]],"date-time":"2020-11-01T00:00:00Z","timestamp":1604188800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["royalsocietypublishing.org"],"crossmark-restriction":true},"short-container-title":["Open Biol."],"published-print":{"date-parts":[[2020,11]]},"abstract":"<jats:p>Infertility affects approximately 15\u201320% of individuals of reproductive age worldwide. Over the last 40 years, assisted reproductive technology (ART) has helped millions of childless couples. However, ART is limited by a low success rate and risk of multiple gestations. Devising methods for selecting the best gamete or embryo that increases the ART success rate and prevention of multiple gestation has become one of the key goals in ART today. Special emphasis has been placed on the development of non-invasive approaches, which do not require perturbing the embryonic cells, as the current morphology-based embryo selection approach has shortcomings in predicting the implantation potential of embryos. An observed association between embryo metabolism and viability has prompted researchers to develop metabolomics-based biomarkers. Nuclear magnetic resonance (NMR) spectroscopy provides a non-invasive approach for the metabolic profiling of tissues, gametes and embryos, with the key advantage of having a minimal sample preparation procedure. Using NMR spectroscopy, biologically important molecules can be identified and quantified in intact cells, extracts or secretomes. This, in turn, helps to map out the active metabolic pathways in a system. The present review covers the contribution of NMR spectroscopy in assisted reproduction at various stages of the process.<\/jats:p>","DOI":"10.1098\/rsob.200092","type":"journal-article","created":{"date-parts":[[2020,11,3]],"date-time":"2020-11-03T19:05:41Z","timestamp":1604430341000},"page":"200092","update-policy":"https:\/\/doi.org\/10.1098\/crossmark-policy","source":"Crossref","is-referenced-by-count":16,"title":["The utility of nuclear magnetic resonance spectroscopy in assisted reproduction"],"prefix":"10.1098","volume":"10","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4930-0349","authenticated-orcid":true,"given":"Gitanjali","family":"Asampille","sequence":"first","affiliation":[{"name":"Department of Clinical Embryology, Kasturba Medical College, Manipal, Manipal Academy of Higher Education, Manipal 576104, India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6172-7367","authenticated-orcid":true,"given":"Aswathi","family":"Cheredath","sequence":"additional","affiliation":[{"name":"Department of Clinical Embryology, Kasturba Medical College, Manipal, Manipal Academy of Higher Education, Manipal 576104, India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4015-9430","authenticated-orcid":true,"given":"David","family":"Joseph","sequence":"additional","affiliation":[{"name":"NMR Research Centre, Indian Institute of Science, Bangalore 560012, India"},{"name":"Solid State and Structural Chemistry Unit, Indian Institute of Science, Bangalore 560012, India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2897-4697","authenticated-orcid":true,"given":"Satish K.","family":"Adiga","sequence":"additional","affiliation":[{"name":"Department of Clinical Embryology, Kasturba Medical College, Manipal, Manipal Academy of Higher Education, Manipal 576104, India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0200-7372","authenticated-orcid":true,"given":"Hanudatta S.","family":"Atreya","sequence":"additional","affiliation":[{"name":"NMR Research Centre, Indian Institute of Science, Bangalore 560012, India"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"175","published-online":{"date-parts":[[2020,11,4]]},"reference":[{"key":"e_1_3_5_2_2","doi-asserted-by":"publisher","DOI":"10.1186\/1477-7827-11-66"},{"key":"e_1_3_5_3_2","doi-asserted-by":"publisher","DOI":"10.1186\/s12958-018-0436-9"},{"key":"e_1_3_5_4_2","doi-asserted-by":"publisher","DOI":"10.1186\/s12958-016-0225-2"},{"key":"e_1_3_5_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(78)92957-4"},{"key":"e_1_3_5_6_2","unstructured":"ESHRE Monitoring. 2018 More than 8 million babies born from IVF since the world's first in 1978. See https:\/\/www.sciencedaily.com\/releases\/2018\/07\/180703084127.htm."},{"key":"e_1_3_5_7_2","unstructured":"Health D of R. 2019 Assisted reproductive technology 2017: fertility clinic success rates report . Atlanta GA: National Centre for Chronic Disease Prevention and Health Promotion."},{"key":"e_1_3_5_8_2","unstructured":"Report NS. 2018 Assisted reproductive technology 2016: national summary report . Atlanta GA: National Centre for Chronic Disease Prevention and Health Promotion."},{"key":"e_1_3_5_9_2","first-page":"1","article-title":"ART in Europe, 2016: results generated from European registries by ESHRE","volume":"3","author":"Wyns C","year":"2020","journal-title":"Hum. Reprod. Open"},{"key":"e_1_3_5_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.fertnstert.2006.11.008"},{"key":"e_1_3_5_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.beem.2019.04.001"},{"key":"e_1_3_5_12_2","doi-asserted-by":"publisher","DOI":"10.1080\/09513590.2020.1737006"},{"key":"e_1_3_5_13_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF01133388"},{"key":"e_1_3_5_14_2","doi-asserted-by":"crossref","first-page":"150","DOI":"10.5935\/1518-0557.20160033","article-title":"Methods for assessing the quality of mammalian embryos: how far we are from the gold standard?","volume":"20","author":"Rocha JC","year":"2016","journal-title":"JBRA Assist. Reprod."},{"key":"e_1_3_5_15_2","doi-asserted-by":"publisher","DOI":"10.1055\/s-0033-1363555"},{"key":"e_1_3_5_16_2","doi-asserted-by":"publisher","DOI":"10.1093\/humrep\/dez064"},{"key":"e_1_3_5_17_2","doi-asserted-by":"publisher","DOI":"10.1093\/molehr\/gan066"},{"key":"e_1_3_5_18_2","doi-asserted-by":"publisher","DOI":"10.1530\/REP-11-0484"},{"key":"e_1_3_5_19_2","doi-asserted-by":"publisher","DOI":"10.1093\/oxfordjournals.humrep.a136376"},{"key":"e_1_3_5_20_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.fertnstert.2007.07.1390"},{"key":"e_1_3_5_21_2","doi-asserted-by":"publisher","DOI":"10.3892\/etm.2017.4160"},{"key":"e_1_3_5_22_2","doi-asserted-by":"publisher","DOI":"10.1530\/REP-12-0168"},{"key":"e_1_3_5_23_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.fertnstert.2008.07.1739"},{"key":"e_1_3_5_24_2","doi-asserted-by":"publisher","DOI":"10.1002\/nbm.2814"},{"key":"e_1_3_5_25_2","doi-asserted-by":"publisher","DOI":"10.1097\/MCO.0000000000000206"},{"key":"e_1_3_5_26_2","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-4939-2377-9_13"},{"key":"e_1_3_5_27_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.copbio.2016.07.006"},{"key":"e_1_3_5_28_2","doi-asserted-by":"publisher","DOI":"10.1021\/np200993k"},{"key":"e_1_3_5_29_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.copbio.2016.08.001"},{"key":"e_1_3_5_30_2","doi-asserted-by":"publisher","DOI":"10.1186\/1741-7015-10-153"},{"key":"e_1_3_5_31_2","doi-asserted-by":"publisher","DOI":"10.1039\/C6MB00420B"},{"key":"e_1_3_5_32_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1472-6483(12)60008-X"},{"key":"e_1_3_5_33_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jmr.2015.07.014"},{"key":"e_1_3_5_34_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.pnmrs.2016.01.005"},{"key":"e_1_3_5_35_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.trac.2018.10.036"},{"key":"e_1_3_5_36_2","doi-asserted-by":"publisher","DOI":"10.3390\/metabo9070123"},{"key":"e_1_3_5_37_2","doi-asserted-by":"publisher","DOI":"10.1021\/ed500324w"},{"key":"e_1_3_5_38_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.physchem.040808.090420"},{"key":"e_1_3_5_39_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.trac.2007.12.001"},{"key":"e_1_3_5_40_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00216-012-6188-z"},{"key":"e_1_3_5_41_2","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-62703-299-5_16"},{"key":"e_1_3_5_42_2","unstructured":"Truong QX Yoon JM Shanks JV. 2014 Isotopomer measurement techniques in metabolic flux analysis I: nuclear magnetic resonance. (doi:10.1007\/978-1-62703-661-0)"},{"key":"e_1_3_5_43_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.plantsci.2012.06.013"},{"key":"e_1_3_5_44_2","first-page":"1","article-title":"Ultra-high field NMR and MRI\u2014the role of magnet technology to increase sensitivity and specificity","volume":"5","author":"Moser E","year":"2017","journal-title":"Front. Phys."},{"key":"e_1_3_5_45_2","doi-asserted-by":"publisher","DOI":"10.1021\/ac025556e"},{"key":"e_1_3_5_46_2","doi-asserted-by":"crossref","unstructured":"Martin GE. 2005 Small-volume and high-sensitivity NMR probes . Paris France: Elsevier Masson SAS.","DOI":"10.1016\/S0066-4103(05)56001-0"},{"key":"e_1_3_5_47_2","doi-asserted-by":"publisher","DOI":"10.1007\/s10858-011-9488-2"},{"key":"e_1_3_5_48_2","doi-asserted-by":"publisher","DOI":"10.1002\/mrc.4236"},{"key":"e_1_3_5_49_2","doi-asserted-by":"publisher","DOI":"10.1093\/humrep\/der037"},{"key":"e_1_3_5_50_2","volume-title":"Basic one- and two-dimensional NMR spectroscopy","author":"Friebolin H","year":"2011","edition":"5"},{"key":"e_1_3_5_51_2","volume-title":"NMR spectroscopy: basic principles, concepts and applications in chemistry","author":"G\u00fcnther H","year":"2013"},{"key":"e_1_3_5_52_2","volume-title":"Understanding NMR spectroscopy","author":"Keeler J","year":"2010"},{"key":"e_1_3_5_53_2","doi-asserted-by":"publisher","DOI":"10.1021\/ac060209g"},{"key":"e_1_3_5_54_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00216-012-5815-z"},{"key":"e_1_3_5_55_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41592-019-0495-7"},{"key":"e_1_3_5_56_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-anchem-071213-020208"},{"key":"e_1_3_5_57_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkn810"},{"key":"e_1_3_5_58_2","doi-asserted-by":"publisher","DOI":"10.1038\/nbt0208-162"},{"key":"e_1_3_5_59_2","doi-asserted-by":"publisher","DOI":"10.1002\/mrc.4263"},{"key":"e_1_3_5_60_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkm957"},{"key":"e_1_3_5_61_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkl923"},{"key":"e_1_3_5_62_2","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/bts308"},{"key":"e_1_3_5_63_2","doi-asserted-by":"crossref","first-page":"e0124219","DOI":"10.1371\/journal.pone.0124219","article-title":"Accurate, fully-automated NMR spectral profiling for metabolomics","volume":"10","author":"Ravanbakhsh S","year":"2015","journal-title":"PLoS ONE"},{"key":"e_1_3_5_64_2","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2105-9-507"},{"key":"e_1_3_5_65_2","doi-asserted-by":"publisher","DOI":"10.3389\/fbioe.2015.00022"},{"key":"e_1_3_5_66_2","doi-asserted-by":"publisher","DOI":"10.1021\/acs.analchem.5b00990"},{"key":"e_1_3_5_67_2","doi-asserted-by":"publisher","DOI":"10.1021\/acs.analchem.5b03082"},{"key":"e_1_3_5_68_2","doi-asserted-by":"publisher","DOI":"10.1021\/ja068541x"},{"key":"e_1_3_5_69_2","doi-asserted-by":"publisher","DOI":"10.1002\/mrc.2519"},{"key":"e_1_3_5_70_2","doi-asserted-by":"publisher","DOI":"10.1021\/ac200007p"},{"key":"e_1_3_5_71_2","doi-asserted-by":"publisher","DOI":"10.1021\/ac403520j"},{"key":"e_1_3_5_72_2","doi-asserted-by":"publisher","DOI":"10.1021\/ac500966e"},{"key":"e_1_3_5_73_2","doi-asserted-by":"publisher","DOI":"10.1007\/s10858-014-9814-6"},{"key":"e_1_3_5_74_2","doi-asserted-by":"publisher","DOI":"10.1007\/s12039-015-0868-0"},{"key":"e_1_3_5_75_2","doi-asserted-by":"publisher","DOI":"10.1002\/mrc.4292"},{"key":"e_1_3_5_76_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jmr.2017.11.005"},{"key":"e_1_3_5_77_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-018-22541-0"},{"key":"e_1_3_5_78_2","doi-asserted-by":"publisher","DOI":"10.1007\/s10858-008-9254-2"},{"key":"e_1_3_5_79_2","doi-asserted-by":"publisher","DOI":"10.1007\/978-94-007-4954-2"},{"key":"e_1_3_5_80_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-017-09884-w"},{"key":"e_1_3_5_81_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0006-3495(98)74071-8"},{"key":"e_1_3_5_82_2","doi-asserted-by":"publisher","DOI":"10.1002\/jcp.1041050310"},{"key":"e_1_3_5_83_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1432-1033.1976.tb10032.x"},{"key":"e_1_3_5_84_2","doi-asserted-by":"publisher","DOI":"10.1007\/s11306-018-1414-0"},{"key":"e_1_3_5_85_2","doi-asserted-by":"publisher","DOI":"10.1093\/humrep\/deu236"},{"key":"e_1_3_5_86_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41746-019-0096-y"},{"key":"e_1_3_5_87_2","doi-asserted-by":"publisher","DOI":"10.1093\/oxfordjournals.humrep.a136905"},{"key":"e_1_3_5_88_2","doi-asserted-by":"publisher","DOI":"10.1093\/humrep\/des175"},{"key":"e_1_3_5_89_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.fertnstert.2018.11.036"},{"key":"e_1_3_5_90_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF01204436"},{"key":"e_1_3_5_91_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0015-0282(01)02888-6"},{"key":"e_1_3_5_92_2","doi-asserted-by":"publisher","DOI":"10.1093\/humrep\/deh409"},{"key":"e_1_3_5_93_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.fertnstert.2009.03.078"},{"key":"e_1_3_5_94_2","first-page":"1","article-title":"Spent embryo culture medium metabolites are related to the in vitro attachment ability of blastocysts","volume":"8","author":"D'Souza F","year":"2018","journal-title":"Sci. Rep."},{"key":"e_1_3_5_95_2","first-page":"321","article-title":"Birth after the reimplantation of a human embryo","volume":"116","author":"Steptoe PC","year":"1978","journal-title":"Arch. Pathol. Lab. Med."},{"key":"e_1_3_5_96_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.bpobgyn.2006.09.009"},{"key":"e_1_3_5_97_2","doi-asserted-by":"publisher","DOI":"10.1093\/humrep\/deg389"},{"key":"e_1_3_5_98_2","doi-asserted-by":"publisher","DOI":"10.1007\/s10815-018-1195-4"},{"key":"e_1_3_5_99_2","doi-asserted-by":"publisher","DOI":"10.1093\/humrep\/dew157"},{"key":"e_1_3_5_100_2","first-page":"451","article-title":"Considerations regarding embryo culture conditions: from media to epigenetics","volume":"32","author":"Simopoulou M","year":"2018","journal-title":"In Vivo"},{"key":"e_1_3_5_101_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.fertnstert.2008.12.030"},{"key":"e_1_3_5_102_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.fertnstert.2017.01.007"},{"key":"e_1_3_5_103_2","doi-asserted-by":"publisher","DOI":"10.1093\/humrep\/dez102"},{"key":"e_1_3_5_104_2","doi-asserted-by":"publisher","DOI":"10.1007\/s11306-010-0245-4"},{"key":"e_1_3_5_105_2","doi-asserted-by":"publisher","DOI":"10.1007\/s10815-012-9877-9"},{"key":"e_1_3_5_106_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.fertnstert.2012.07.1145"},{"key":"e_1_3_5_107_2","doi-asserted-by":"publisher","DOI":"10.1007\/s10334-012-0331-x"},{"key":"e_1_3_5_108_2","doi-asserted-by":"publisher","DOI":"10.3109\/19396368.2013.854426"},{"key":"e_1_3_5_109_2","doi-asserted-by":"publisher","DOI":"10.1080\/19396368.2016.1217952"},{"key":"e_1_3_5_110_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.repbio.2016.07.004"},{"key":"e_1_3_5_111_2","doi-asserted-by":"publisher","DOI":"10.3934\/bioeng.2016.4.538"},{"key":"e_1_3_5_112_2","doi-asserted-by":"publisher","DOI":"10.1017\/S0967199417000417"},{"key":"e_1_3_5_113_2","doi-asserted-by":"publisher","DOI":"10.1038\/srep37291"},{"key":"e_1_3_5_114_2","doi-asserted-by":"publisher","DOI":"10.1071\/RD18136"},{"key":"e_1_3_5_115_2","doi-asserted-by":"publisher","DOI":"10.1002\/nbm.1488"},{"key":"e_1_3_5_116_2","doi-asserted-by":"publisher","DOI":"10.1089\/omi.2014.0097"},{"key":"e_1_3_5_117_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.fertnstert.2012.01.122"},{"key":"e_1_3_5_118_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.fertnstert.2012.07.1131"},{"key":"e_1_3_5_119_2","doi-asserted-by":"publisher","DOI":"10.18632\/oncotarget.19058"},{"key":"e_1_3_5_120_2","first-page":"256","article-title":"1H NMR-based metabolomics study on follicular fluid from patients with polycystic ovary syndrome","volume":"43","author":"Jaccarino N","year":"2019","journal-title":"Biochim. Clin"},{"key":"e_1_3_5_121_2","doi-asserted-by":"publisher","DOI":"10.1039\/C7MB00181A"},{"key":"e_1_3_5_122_2","doi-asserted-by":"publisher","DOI":"10.1007\/s10815-018-1281-7"},{"key":"e_1_3_5_123_2","doi-asserted-by":"publisher","DOI":"10.1080\/19396368.2018.1478469"},{"key":"e_1_3_5_124_2","doi-asserted-by":"publisher","DOI":"10.1007\/s11306-019-1481-x"},{"key":"e_1_3_5_125_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF01940658"},{"key":"e_1_3_5_126_2","doi-asserted-by":"publisher","DOI":"10.1530\/rep.0.1240241"},{"key":"e_1_3_5_127_2","doi-asserted-by":"publisher","DOI":"10.1530\/REP-13-0142"},{"key":"e_1_3_5_128_2","doi-asserted-by":"publisher","DOI":"10.1093\/humrep\/dew064"},{"key":"e_1_3_5_129_2","first-page":"14","article-title":"Investigation into the use of nuclear magnetic resonance spectroscopy for viability predictions, in real time on mouse preimplantation embryos","volume":"2","author":"Lyman JT","year":"2016","journal-title":"Int. J. New Technol. Res."},{"key":"e_1_3_5_130_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jmr.2008.03.006"},{"key":"e_1_3_5_131_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jep.2019.111839"},{"key":"e_1_3_5_132_2","doi-asserted-by":"publisher","DOI":"10.1148\/radiology.177.3.2243981"},{"key":"e_1_3_5_133_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0014-5793(00)02307-3"},{"key":"e_1_3_5_134_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbadis.2018.07.028"},{"key":"e_1_3_5_135_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.reprotox.2009.03.005"},{"key":"e_1_3_5_136_2","doi-asserted-by":"publisher","DOI":"10.1111\/andr.12027"},{"key":"e_1_3_5_137_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-017-04146-1"},{"key":"e_1_3_5_138_2","doi-asserted-by":"publisher","DOI":"10.1093\/molehr\/gax025"},{"key":"e_1_3_5_139_2","doi-asserted-by":"publisher","DOI":"10.1093\/molehr\/gay046"},{"key":"e_1_3_5_140_2","doi-asserted-by":"publisher","DOI":"10.1016\/0014-4827(83)90400-7"},{"key":"e_1_3_5_141_2","doi-asserted-by":"publisher","DOI":"10.1002\/jez.1402380111"},{"key":"e_1_3_5_142_2","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-010X(20000401)286:5<513::AID-JEZ9>3.0.CO;2-5"},{"key":"e_1_3_5_143_2","doi-asserted-by":"publisher","DOI":"10.1139\/o87-061"},{"key":"e_1_3_5_144_2","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod33.5.1029"},{"key":"e_1_3_5_145_2","doi-asserted-by":"publisher","DOI":"10.1016\/0731-7085(94)80004-9"},{"key":"e_1_3_5_146_2","doi-asserted-by":"publisher","DOI":"10.1093\/humrep\/13.1.132"},{"key":"e_1_3_5_147_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0304-4165(97)00116-5"},{"key":"e_1_3_5_148_2","doi-asserted-by":"publisher","DOI":"10.1530\/rep.0.1220431"},{"key":"e_1_3_5_149_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jpba.2011.03.010"},{"key":"e_1_3_5_150_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jpba.2010.07.021"},{"key":"e_1_3_5_151_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jep.2013.06.024"},{"key":"e_1_3_5_152_2","doi-asserted-by":"publisher","DOI":"10.3109\/19396368.2015.1054003"},{"key":"e_1_3_5_153_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cca.2015.09.001"},{"key":"e_1_3_5_154_2","doi-asserted-by":"publisher","DOI":"10.1007\/s10815-018-1350-y"},{"key":"e_1_3_5_155_2","doi-asserted-by":"publisher","DOI":"10.1111\/andr.12707"},{"key":"e_1_3_5_156_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jpba.2019.112903"},{"key":"e_1_3_5_157_2","doi-asserted-by":"publisher","DOI":"10.1080\/19396368.2017.1295332"},{"key":"e_1_3_5_158_2","doi-asserted-by":"publisher","DOI":"10.1093\/humrep\/dez085"},{"key":"e_1_3_5_159_2","doi-asserted-by":"publisher","DOI":"10.1002\/cphc.200800461"},{"key":"e_1_3_5_160_2","doi-asserted-by":"crossref","unstructured":"Adams RW. 2014 Pure shift nmr spectroscopy. In eMagRes pp. 295\u2013310. Chichester UK: John Wiley & Sons.","DOI":"10.1002\/9780470034590.emrstm1362"},{"key":"e_1_3_5_161_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.pnmrs.2015.02.002"},{"key":"e_1_3_5_162_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1359-6446(03)02749-1"},{"key":"e_1_3_5_163_2","doi-asserted-by":"publisher","DOI":"10.1517\/17425255.4.10.1295"},{"key":"e_1_3_5_164_2","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1522-2594(199906)41:6<1108::AID-MRM6>3.0.CO;2-M"},{"key":"e_1_3_5_165_2","first-page":"1890","article-title":"Melanoma tumors acquire a new phospholipid metabolism phenotype under cystemustine as revealed by high-resolution magic angle spinning proton nuclear magnetic resonance spectroscopy of intact tumor samples","volume":"62","author":"Morvan D","year":"2002","journal-title":"Cancer Res."},{"key":"e_1_3_5_166_2","first-page":"446","volume-title":"Methodologies for metabolomics","author":"Hurd RE","year":"2010"},{"key":"e_1_3_5_167_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1733835100"},{"key":"e_1_3_5_168_2","doi-asserted-by":"crossref","first-page":"385","DOI":"10.1007\/978-1-4939-9690-2_21","volume-title":"NMR-based metabolomics: methods and protocols","author":"Lerche MH","year":"2019"},{"key":"e_1_3_5_169_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M113.498626"},{"key":"e_1_3_5_170_2","first-page":"1000e116","article-title":"Novel drug therapies and diagnostics for personalized medicine and nanomedicine in genome science, nanoscience, and molecular engineering","volume":"1","author":"Lin E","year":"2012","journal-title":"Pharm. Regul. Aff. Open Access"}],"container-title":["Open Biology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/royalsocietypublishing.org\/doi\/pdf\/10.1098\/rsob.200092","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/royalsocietypublishing.org\/doi\/full-xml\/10.1098\/rsob.200092","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/royalsocietypublishing.org\/doi\/pdf\/10.1098\/rsob.200092","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,11,26]],"date-time":"2022-11-26T07:43:44Z","timestamp":1669448624000},"score":1,"resource":{"primary":{"URL":"https:\/\/royalsocietypublishing.org\/doi\/10.1098\/rsob.200092"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,11]]},"references-count":169,"journal-issue":{"issue":"11","published-print":{"date-parts":[[2020,11]]}},"alternative-id":["10.1098\/rsob.200092"],"URL":"https:\/\/doi.org\/10.1098\/rsob.200092","relation":{"has-review":[{"id-type":"doi","id":"10.1098\/RSOB.200092\/v1\/review1","asserted-by":"object"},{"id-type":"doi","id":"10.1098\/RSOB.200092\/v2\/response1","asserted-by":"object"},{"id-type":"doi","id":"10.1098\/RSOB.200092\/v1\/review2","asserted-by":"object"},{"id-type":"doi","id":"10.1098\/RSOB.200092\/v2\/decision1","asserted-by":"object"},{"id-type":"doi","id":"10.1098\/RSOB.200092\/v3\/decision1","asserted-by":"object"},{"id-type":"doi","id":"10.1098\/RSOB.200092\/v2\/review2","asserted-by":"object"},{"id-type":"doi","id":"10.1098\/RSOB.200092\/v1\/decision1","asserted-by":"object"},{"id-type":"doi","id":"10.1098\/RSOB.200092\/v2\/review1","asserted-by":"object"},{"id-type":"doi","id":"10.1098\/RSOB.200092\/v4\/decision1","asserted-by":"object"},{"id-type":"doi","id":"10.1098\/RSOB.200092\/v4\/response1","asserted-by":"object"},{"id-type":"doi","id":"10.1098\/RSOB.200092\/v3\/review1","asserted-by":"object"},{"id-type":"doi","id":"10.1098\/RSOB.200092\/v3\/response1","asserted-by":"object"}]},"ISSN":["2046-2441"],"issn-type":[{"value":"2046-2441","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,11]]},"assertion":[{"value":"2020-04-11","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-10-13","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-11-04","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}