{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,21]],"date-time":"2026-07-21T10:56:33Z","timestamp":1784631393162,"version":"3.55.0"},"reference-count":45,"publisher":"The Royal Society","issue":"2","license":[{"start":{"date-parts":[[2021,2,1]],"date-time":"2021-02-01T00:00:00Z","timestamp":1612137600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/royalsociety.org\/journals\/ethics-policies\/data-sharing-mining\/"}],"funder":[{"name":"JSPS"},{"name":"Japanese Government"},{"DOI":"10.13039\/501100001691","name":"Grants-in-Aid for Scientific Research","doi-asserted-by":"crossref","award":["18H02568"],"award-info":[{"award-number":["18H02568"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100001691","name":"Grants-in-Aid for Scientific Research","doi-asserted-by":"crossref","award":["19K06454"],"award-info":[{"award-number":["19K06454"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["royalsocietypublishing.org"],"crossmark-restriction":true},"short-container-title":["Open Biol."],"published-print":{"date-parts":[[2021,2]]},"abstract":"<jats:p>\n                    Polypyrrole-based polyamides are used as sequence-specific DNA probes. However, their cellular uptake and distribution are affected by several factors and have not been extensively studied\n                    <jats:italic>in vivo<\/jats:italic>\n                    . Here, we generated a series of fluorescence-conjugated polypyrrole compounds and examined their cellular distribution using live zebrafish and cultured human cells. Among the evaluated compounds, Py\n                    <jats:sub>3<\/jats:sub>\n                    -FITC was able to visualize collagen-rich tissues, such as the jaw cartilage, opercle and bulbus arteriosus, in early-stage living zebrafish embryos. Then, we stained cultured human cells with Py\n                    <jats:sub>3<\/jats:sub>\n                    -FITC and found that the staining became more intense as the amount of collagen was increased. In addition, Py\n                    <jats:sub>3<\/jats:sub>\n                    -FITC-stained HR cells, which represent a type of ionocyte on the body surface of living zebrafish embryos. Py\n                    <jats:sub>3<\/jats:sub>\n                    -FITC has low toxicity, and collagen-rich tissues and ionocytes can be visualized when soaked in Py\n                    <jats:sub>3<\/jats:sub>\n                    -FITC solution. Therefore, Py\n                    <jats:sub>3<\/jats:sub>\n                    -FITC may be a useful live imaging tool for detecting changes in collagen-rich tissue and ionocytes, including their mammalian analogues, during both normal development and disease progression.\n                  <\/jats:p>","DOI":"10.1098\/rsob.200241","type":"journal-article","created":{"date-parts":[[2021,2,9]],"date-time":"2021-02-09T19:12:20Z","timestamp":1612897940000},"update-policy":"https:\/\/doi.org\/10.1098\/crossmark-policy","source":"Crossref","is-referenced-by-count":6,"title":["Py\n                    <sub>3<\/sub>\n                    -FITC: a new fluorescent probe for live cell imaging of collagen-rich tissues and ionocytes"],"prefix":"10.1098","volume":"11","author":[{"given":"Zhaotong","family":"Wang","sequence":"first","affiliation":[{"name":"Graduate School of Pharmaceutical Sciences, Chiba University, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Takamasa","family":"Mizoguchi","sequence":"additional","affiliation":[{"name":"Graduate School of Pharmaceutical Sciences, Chiba University, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Takahito","family":"Kuribara","sequence":"additional","affiliation":[{"name":"Graduate School of Pharmaceutical Sciences, Chiba University, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Masaya","family":"Nakajima","sequence":"additional","affiliation":[{"name":"Graduate School of Pharmaceutical Sciences, Chiba University, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mayuu","family":"Iwata","sequence":"additional","affiliation":[{"name":"Graduate School of Pharmaceutical Sciences, Chiba University, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuka","family":"Sakamoto","sequence":"additional","affiliation":[{"name":"Graduate School of Pharmaceutical Sciences, Chiba University, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hiroyuki","family":"Nakamura","sequence":"additional","affiliation":[{"name":"Graduate School of Pharmaceutical Sciences, Chiba University, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Toshihiko","family":"Murayama","sequence":"additional","affiliation":[{"name":"Graduate School of Pharmaceutical Sciences, Chiba University, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tetsuhiro","family":"Nemoto","sequence":"additional","affiliation":[{"name":"Graduate School of Pharmaceutical Sciences, Chiba University, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5017-2482","authenticated-orcid":true,"given":"Motoyuki","family":"Itoh","sequence":"additional","affiliation":[{"name":"Graduate School of Pharmaceutical Sciences, Chiba University, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"175","published-online":{"date-parts":[[2021,2,10]]},"reference":[{"key":"e_1_3_7_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/0079-6107(86)90005-2"},{"key":"e_1_3_7_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0968-0896(00)00219-4"},{"key":"e_1_3_7_4_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.2035074100"},{"key":"e_1_3_7_5_2","doi-asserted-by":"publisher","DOI":"10.18632\/oncotarget.25451"},{"key":"e_1_3_7_6_2","doi-asserted-by":"publisher","DOI":"10.1021\/ja960720z"},{"key":"e_1_3_7_7_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41591-019-0705-y"},{"key":"e_1_3_7_8_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.biochem.77.032207.120833"},{"key":"e_1_3_7_9_2","doi-asserted-by":"publisher","DOI":"10.1038\/nm.2807"},{"key":"e_1_3_7_10_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0832308100"},{"key":"e_1_3_7_11_2","doi-asserted-by":"publisher","DOI":"10.1038\/nbt894"},{"key":"e_1_3_7_12_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0114983"},{"key":"e_1_3_7_13_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.yexcr.2014.05.005"},{"key":"e_1_3_7_14_2","doi-asserted-by":"publisher","DOI":"10.1002\/cyto.a.22264"},{"key":"e_1_3_7_15_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ydbio.2018.06.001"},{"key":"e_1_3_7_16_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ygcen.2019.03.007"},{"key":"e_1_3_7_17_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ydbio.2009.02.026"},{"key":"e_1_3_7_18_2","doi-asserted-by":"publisher","DOI":"10.1152\/ajpregu.00200.2006"},{"key":"e_1_3_7_19_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ydbio.2009.02.031"},{"key":"e_1_3_7_20_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.stem.2007.11.002"},{"key":"e_1_3_7_21_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1534-5807(02)00409-4"},{"key":"e_1_3_7_22_2","unstructured":"Westerfield M. 2020 The zebrafish book: a guide for the laboratory use of zebrafish (Danio rerio) 4th edition. 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