{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,26]],"date-time":"2026-09-26T02:21:37Z","timestamp":1790389297519,"version":"4.1.0"},"reference-count":73,"publisher":"The Royal Society","issue":"11","license":[{"start":{"date-parts":[[2021,11,1]],"date-time":"2021-11-01T00:00:00Z","timestamp":1635724800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/royalsociety.org\/journals\/ethics-policies\/data-sharing-mining\/"}],"funder":[{"name":"INRAE\/R\u00e9gion Centre doctoral Fellowship"},{"DOI":"10.13039\/100010661","name":"Horizon 2020 Framework Programme","doi-asserted-by":"crossref","award":["773830"],"award-info":[{"award-number":["773830"]}],"id":[{"id":"10.13039\/100010661","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["royalsocietypublishing.org"],"crossmark-restriction":true},"short-container-title":["Open Biol."],"published-print":{"date-parts":[[2021,11]]},"abstract":"<jats:p>\n                    Poultry are the main source of human infection by\n                    <jats:italic>Salmonella<\/jats:italic>\n                    . As infected poultry are asymptomatic, identifying infected poultry farms is difficult, thus controlling animal infections is of primary importance. As cell tropism is known to govern disease, our aim was therefore to identify infected host\u2013cell types in the organs of chicks known to be involved in\n                    <jats:italic>Salmonella<\/jats:italic>\n                    infection and investigate the role of the three known invasion factors in this process (T3SS-1, Rck and PagN). Chicks were inoculated with wild-type or isogenic fluorescent\n                    <jats:italic>Salmonella<\/jats:italic>\n                    Typhimurium mutants via the intracoelomic route. Our results show that liver, spleen, gall bladder and aortic vessels could be foci of infection, and that phagocytic and non-phagocytic cells, including immune, epithelial and endothelial cells, are invaded\n                    <jats:italic>in vivo<\/jats:italic>\n                    in each organ. Moreover, a mutant defective for the T3SS-1, Rck and PagN remained able to colonize organs like the wild-type strain and invaded non-phagocytic cells in each organ studied. As the infection of the gall bladder had not previously been described in chicks, invasion of gall bladder cells was confirmed by immunohistochemistry and infection was shown to last several weeks after inoculation. Altogether, for the first time these findings provide insights into cell tropism of\n                    <jats:italic>Salmonella<\/jats:italic>\n                    in relevant organs involved in\n                    <jats:italic>Salmonella<\/jats:italic>\n                    infection in chicks and also demonstrate that the known invasion factors are not required for entry into these cell types.\n                  <\/jats:p>","DOI":"10.1098\/rsob.210117","type":"journal-article","created":{"date-parts":[[2021,11,17]],"date-time":"2021-11-17T03:07:01Z","timestamp":1637118421000},"update-policy":"https:\/\/doi.org\/10.1098\/crossmark-policy","source":"Crossref","is-referenced-by-count":6,"title":["A large panel of chicken cells are invaded\n                    <i>in vivo<\/i>\n                    by\n                    <i>Salmonella<\/i>\n                    Typhimurium even when depleted of all known invasion factors"],"prefix":"10.1098","volume":"11","author":[{"given":"S. M.","family":"Roche","sequence":"first","affiliation":[{"name":"INRAE, Universit\u00e9 de Tours, ISP, 37380 Nouzilly, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7655-3144","authenticated-orcid":true,"given":"S.","family":"Holbert","sequence":"additional","affiliation":[{"name":"INRAE, Universit\u00e9 de Tours, ISP, 37380 Nouzilly, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Y.","family":"Le Vern","sequence":"additional","affiliation":[{"name":"INRAE, Universit\u00e9 de Tours, ISP, 37380 Nouzilly, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C.","family":"Rossignol","sequence":"additional","affiliation":[{"name":"INRAE, Universit\u00e9 de Tours, ISP, 37380 Nouzilly, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A.","family":"Rossignol","sequence":"additional","affiliation":[{"name":"INRAE, Universit\u00e9 de Tours, ISP, 37380 Nouzilly, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8201-0194","authenticated-orcid":true,"given":"P.","family":"Velge","sequence":"additional","affiliation":[{"name":"INRAE, Universit\u00e9 de Tours, ISP, 37380 Nouzilly, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4149-0168","authenticated-orcid":true,"given":"I.","family":"Virlogeux-Payant","sequence":"additional","affiliation":[{"name":"INRAE, Universit\u00e9 de Tours, ISP, 37380 Nouzilly, France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"175","published-online":{"date-parts":[[2021,11,17]]},"reference":[{"key":"e_1_3_5_2_2","unstructured":"WHO. 2017 WHO estimates of the global burden of foodborne diseases. See https:\/\/apps.who.int\/iris\/handle\/10665\/199350."},{"key":"e_1_3_5_3_2","first-page":"5077","article-title":"The European Union summary report on trends and sources of zoonoses, zoonotic agents and food-borne outbreaks in 2016","volume":"15","author":"ECDC Ea","year":"2019","unstructured":"ECDC Ea. 2019 The European Union summary report on trends and sources of zoonoses, zoonotic agents and food-borne outbreaks in 2016. EFSA J. 15, 5077. (doi:10.2903\/j.efsa.2010.1496)","journal-title":"EFSA J."},{"key":"e_1_3_5_4_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.00591-19"},{"key":"e_1_3_5_5_2","volume-title":"Human-clinical-medicine: clinical approaches to zoonoses, toxicants and other shared health risks","author":"Rabinowitz PM","year":"2009","unstructured":"Rabinowitz PM, Conti LA. 2009 Human-clinical-medicine: clinical approaches to zoonoses, toxicants and other shared health risks. 1st edn. Maryland Heights, MD: Saunders.","edition":"1"},{"key":"e_1_3_5_6_2","doi-asserted-by":"publisher","DOI":"10.20506\/rst.29.3.1994"},{"key":"e_1_3_5_7_2","doi-asserted-by":"publisher","DOI":"10.1111\/1462-2920.14294"},{"key":"e_1_3_5_8_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1462-5822.2007.00992.x"},{"key":"e_1_3_5_9_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.62.11.5183-5186.1994"},{"key":"e_1_3_5_10_2","doi-asserted-by":"publisher","DOI":"10.1038\/cr.2010.45"},{"key":"e_1_3_5_11_2","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2180-8-142"},{"key":"e_1_3_5_12_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6968.2009.01666.x"},{"key":"e_1_3_5_13_2","doi-asserted-by":"publisher","DOI":"10.1096\/fj.201600701R"},{"key":"e_1_3_5_14_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.2000.01892.x"},{"key":"e_1_3_5_15_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0005207"},{"key":"e_1_3_5_16_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.86.16.6383"},{"key":"e_1_3_5_17_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.68.9.5050-5055.2000"},{"key":"e_1_3_5_18_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.174.3.1675"},{"key":"e_1_3_5_19_2","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2180-8-182"},{"key":"e_1_3_5_20_2","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2180-9-268"},{"key":"e_1_3_5_21_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.00039-09"},{"key":"e_1_3_5_22_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.01255-07"},{"key":"e_1_3_5_23_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0058449"},{"key":"e_1_3_5_24_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.95.8.4641"},{"key":"e_1_3_5_25_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.01139-09"},{"key":"e_1_3_5_26_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1016180108"},{"key":"e_1_3_5_27_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1006270"},{"key":"e_1_3_5_28_2","doi-asserted-by":"publisher","DOI":"10.1098\/rsob.190036"},{"key":"e_1_3_5_29_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0913087107"},{"key":"e_1_3_5_30_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.1996.00120.x"},{"key":"e_1_3_5_31_2","doi-asserted-by":"publisher","DOI":"10.1016\/0022-2836(80)90283-1"},{"key":"e_1_3_5_32_2","doi-asserted-by":"publisher","DOI":"10.3389\/fcimb.2018.00273"},{"key":"e_1_3_5_33_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0165-2427(97)00152-9"},{"key":"e_1_3_5_34_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.164.1.375"},{"key":"e_1_3_5_35_2","volume-title":"Poultry immunology","author":"Rothwell CJ","year":"1996","unstructured":"Rothwell CJ, Vervelde L, Davison TF. 1996 Identification of Bu-1 alloantigens using the monoclonal antibody AV20. In Poultry immunology. Abindon, UK: Carfax."},{"key":"e_1_3_5_36_2","doi-asserted-by":"publisher","DOI":"10.1006\/excr.1994.1191"},{"key":"e_1_3_5_37_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.80.4.1038"},{"key":"e_1_3_5_38_2","doi-asserted-by":"publisher","DOI":"10.1637\/0005-2086(2003)047[0163:LPOALP]2.0.CO;2"},{"key":"e_1_3_5_39_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.72.4.2152-2159.2004"},{"key":"e_1_3_5_40_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.00258-13"},{"key":"e_1_3_5_41_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrmicro2490"},{"key":"e_1_3_5_42_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.cellbio.17.1.53"},{"key":"e_1_3_5_43_2","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.00013-07"},{"key":"e_1_3_5_44_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.mib.2008.12.001"},{"key":"e_1_3_5_45_2","doi-asserted-by":"publisher","DOI":"10.1186\/1297-9716-42-16"},{"key":"e_1_3_5_46_2","doi-asserted-by":"publisher","DOI":"10.4161\/gmic.1.1.10950"},{"key":"e_1_3_5_47_2","doi-asserted-by":"publisher","DOI":"10.3389\/fcimb.2018.00318"},{"key":"e_1_3_5_48_2","doi-asserted-by":"publisher","DOI":"10.3389\/fcimb.2019.00270"},{"key":"e_1_3_5_49_2","doi-asserted-by":"publisher","DOI":"10.1186\/s13567-015-0224-x"},{"key":"e_1_3_5_50_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.186.4.569"},{"key":"e_1_3_5_51_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1462-5822.2001.00137.x"},{"key":"e_1_3_5_52_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1462-5822.2003.00296.x"},{"key":"e_1_3_5_53_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.0030196"},{"key":"e_1_3_5_54_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.mimet.2007.08.016"},{"key":"e_1_3_5_55_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1462-5822.2010.01511.x"},{"key":"e_1_3_5_56_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrmicro2034"},{"key":"e_1_3_5_57_2","doi-asserted-by":"publisher","DOI":"10.1016\/0923-2494(91)90090-6"},{"key":"e_1_3_5_58_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.83.14.5189"},{"key":"e_1_3_5_59_2","doi-asserted-by":"publisher","DOI":"10.3390\/ani9020063"},{"key":"e_1_3_5_60_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.1800504"},{"key":"e_1_3_5_61_2","doi-asserted-by":"publisher","DOI":"10.1053\/gast.2001.20947"},{"key":"e_1_3_5_62_2","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2019.01582"},{"key":"e_1_3_5_63_2","doi-asserted-by":"publisher","DOI":"10.17221\/5954-VETMED"},{"key":"e_1_3_5_64_2","volume-title":"Statpearls (internet)","author":"Tucker WD","year":"2020","unstructured":"Tucker WD, Arora Y, Mahajan K. 2020 Anatomy, blood vessels. In Statpearls (internet). Treasure Island, FL: StatPearls Publishing."},{"key":"e_1_3_5_65_2","first-page":"284","article-title":"Gross anatomical and histological studies on the liver of broiler","volume":"4","author":"Iqbal J","year":"2014","unstructured":"Iqbal J, Bhutto AL, Shah MG, Lochi GM, Hayat S, Ali N, Khan T, Khan AM, Khan A. 2014 Gross anatomical and histological studies on the liver of broiler. J. Appl. Environ. Biol. Sci. 4, 284-295.","journal-title":"J. Appl. Environ. Biol. Sci."},{"key":"e_1_3_5_66_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1000862107"},{"key":"e_1_3_5_67_2","doi-asserted-by":"publisher","DOI":"10.3390\/ijms18091887"},{"key":"e_1_3_5_68_2","doi-asserted-by":"publisher","DOI":"10.1086\/646608"},{"key":"e_1_3_5_69_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.tim.2014.06.007"},{"key":"e_1_3_5_70_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.chom.2015.05.002"},{"issue":"5","key":"e_1_3_5_71_2","first-page":"806","article-title":"The guinea pig as a model for the asymptomatic human typhoid carrier","volume":"27","author":"Lavergne GM","year":"1977","unstructured":"Lavergne GM, James HF, Martineau C, Diena BB, Lior H. 1977 The guinea pig as a model for the asymptomatic human typhoid carrier. Lab. Anim. Sci. 27(5 Pt 2), 806-816.","journal-title":"Lab. Anim. Sci."},{"key":"e_1_3_5_72_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.molimm.2008.12.015"},{"key":"e_1_3_5_73_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.01389-09"},{"key":"e_1_3_5_74_2","doi-asserted-by":"crossref","unstructured":"Roche SM Holbert S Vern YL Rossignol C Rossignol A Velge P Virlogeux-Payant I. 2021 A large panel of chicken cells are invaded in vivo by Salmonella Typhimurium even when depleted of all known invasion factors. FigShare .","DOI":"10.1101\/2020.11.17.386375"}],"container-title":["Open Biology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/royalsocietypublishing.org\/doi\/pdf\/10.1098\/rsob.210117","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/royalsocietypublishing.org\/doi\/full-xml\/10.1098\/rsob.210117","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/royalsocietypublishing.org\/doi\/pdf\/10.1098\/rsob.210117","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,5,23]],"date-time":"2023-05-23T09:51:08Z","timestamp":1684835468000},"score":1,"resource":{"primary":{"URL":"https:\/\/royalsocietypublishing.org\/doi\/10.1098\/rsob.210117"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,11]]},"references-count":73,"journal-issue":{"issue":"11","published-print":{"date-parts":[[2021,11]]}},"alternative-id":["10.1098\/rsob.210117"],"URL":"https:\/\/doi.org\/10.1098\/rsob.210117","relation":{"has-preprint":[{"id-type":"doi","id":"10.1101\/2020.11.17.386375","asserted-by":"object"}],"has-review":[{"id-type":"doi","id":"10.1098\/RSOB.210117\/v2\/response1","asserted-by":"object"},{"id-type":"doi","id":"10.1098\/RSOB.210117\/v3\/decision1","asserted-by":"object"},{"id-type":"doi","id":"10.1098\/RSOB.210117\/v2\/decision1","asserted-by":"object"},{"id-type":"doi","id":"10.1098\/RSOB.210117\/v1\/review1","asserted-by":"object"},{"id-type":"doi","id":"10.1098\/RSOB.210117\/v1\/decision1","asserted-by":"object"},{"id-type":"doi","id":"10.1098\/RSOB.210117\/v2\/review1","asserted-by":"object"},{"id-type":"doi","id":"10.1098\/RSOB.210117\/v3\/review1","asserted-by":"object"}]},"ISSN":["2046-2441"],"issn-type":[{"value":"2046-2441","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,11]]},"assertion":[{"value":"2021-06-02","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2021-10-05","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2021-11-17","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"210117"}}