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This is the first study evaluating tRNA synthetases as potential drug targets in ticks. RNAi knock-down of all tested tRNA synthetases had a strong deleterious phenotype on Ixodes ricinus feeding. Our data indicate that tRNA synthetases represent attractive, anti-tick targets warranting the design of selective inhibitors. Further, we tested whether these severely impaired ticks were capable of transmitting Borrelia afzelii spirochaetes. Interestingly, biologically handicapped I. ricinus nymphs transmitted B. afzelii in a manner quantitatively sufficient to develop a systemic infection in mice. These data suggest that initial blood-feeding, despite the incapability of ticks to fully feed and salivate, is sufficient for activating B. afzelii from a dormant to an infectious mode, enabling transmission and dissemination in host tissues.<\/jats:p>","DOI":"10.1098\/rsob.210244","type":"journal-article","created":{"date-parts":[[2022,2,15]],"date-time":"2022-02-15T19:05:15Z","timestamp":1644951915000},"update-policy":"https:\/\/doi.org\/10.1098\/crossmark-policy","source":"Crossref","is-referenced-by-count":7,"title":["Lyme disease transmission by severely impaired ticks"],"prefix":"10.1098","volume":"12","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7719-4251","authenticated-orcid":true,"given":"Jan","family":"Perner","sequence":"first","affiliation":[{"name":"Ceske Budejovice 37005, Czech Republic","department":["Institute of Parasitology, Biology Centre of the Czech Academy of Sciences,"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Matej","family":"Kucera","sequence":"additional","affiliation":[{"name":"Ceske Budejovice 37005, Czech Republic","department":["Institute of Parasitology, Biology Centre of the Czech Academy of Sciences,"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8462-0914","authenticated-orcid":true,"given":"Helena","family":"Frantova","sequence":"additional","affiliation":[{"name":"Ceske Budejovice 37005, Czech Republic","department":["Institute of Parasitology, Biology Centre of the Czech Academy of Sciences,"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9630-4516","authenticated-orcid":true,"given":"Veronika","family":"Urbanova","sequence":"additional","affiliation":[{"name":"Ceske Budejovice 37005, Czech Republic","department":["Institute of Parasitology, Biology Centre of the Czech Academy of Sciences,"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2412-5724","authenticated-orcid":true,"given":"Petr","family":"Kopacek","sequence":"additional","affiliation":[{"name":"Ceske Budejovice 37005, Czech Republic","department":["Institute of Parasitology, Biology Centre of the Czech Academy of Sciences,"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5058-4476","authenticated-orcid":true,"given":"Radek","family":"Sima","sequence":"additional","affiliation":[{"name":"Ceske Budejovice 37005, Czech Republic","department":["Institute of Parasitology, Biology Centre of the Czech Academy of Sciences,"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"175","published-online":{"date-parts":[[2022,2,16]]},"reference":[{"key":"2026022416363793000_RSOB210244C1","doi-asserted-by":"crossref","first-page":"e113294","DOI":"10.1371\/journal.pone.0113294","article-title":"Design and development of a novel vaccine for protection against Lyme borreliosis","volume":"9","author":"Comstedt","year":"2014","journal-title":"PLoS ONE"},{"key":"2026022416363793000_RSOB210244C2","doi-asserted-by":"crossref","first-page":"33","DOI":"10.1038\/s41541-020-0183-8","article-title":"Design of a broadly reactive Lyme disease vaccine","volume":"5","author":"Kamp","year":"2020","journal-title":"NPJ Vaccines"},{"key":"2026022416363793000_RSOB210244C3","doi-asserted-by":"crossref","first-page":"680","DOI":"10.1016\/S1473-3099(13)70110-5","article-title":"Safety and immunogenicity of a novel multivalent OspA vaccine against Lyme borreliosis in healthy adults: a double-blind, randomised, dose-escalation phase 1\/2 trial","volume":"13","author":"Wressnigg","year":"2013","journal-title":"Lancet Infect. 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