{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,27]],"date-time":"2026-09-27T10:24:49Z","timestamp":1790504689703,"version":"4.1.0"},"reference-count":46,"publisher":"The Royal Society","issue":"3","license":[{"start":{"date-parts":[[2022,3,1]],"date-time":"2022-03-01T00:00:00Z","timestamp":1646092800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/royalsociety.org\/journals\/ethics-policies\/data-sharing-mining\/"}],"funder":[{"DOI":"10.13039\/501100001713","name":"European & Developing Countries Clinical Trials Partnership","doi-asserted-by":"crossref","award":["TA.2010.40200.027"],"award-info":[{"award-number":["TA.2010.40200.027"]}],"id":[{"id":"10.13039\/501100001713","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["royalsocietypublishing.org"],"crossmark-restriction":true},"short-container-title":["Open Biol."],"published-print":{"date-parts":[[2022,3]]},"abstract":"<jats:p>\n                    The antibody-dependent respiratory burst (ADRB) assay is a sensitive isoluminol-based chemiluminescence (CL) functional assay designed to assess the capacity of opsonizing antibodies against merozoites to induce neutrophil respiratory burst. ADRB was shown to measure protective immunity against malaria in endemic areas, but the assay needed further improvement to ensure better sensitivity and reproducibility. Here, we adjusted parameters such as the freezing\u2013thawing procedure of merozoites, merozoites's concentration and the buffer solution's pH, and we used the improved assay to measure ADRB activity of 207 sera from 97 and 110 individuals living, respectively, in Dielmo and Ndiop villages with differing malaria endemicity. The improvement led to increased CL intensity and assay sensitivity, and a higher reproducibility. In both areas, ADRB activity correlated with malaria endemicity and individual's age discriminated groups with and without clinical malaria episodes, and significantly correlated with\n                    <jats:italic>in vivo<\/jats:italic>\n                    clinical protection from\n                    <jats:italic>Plasmodium falciparum<\/jats:italic>\n                    malaria. Our results demonstrate that the improved ADRB assay can be valuably used to assess acquired immunity during monitoring by control programmes and\/or clinical trials.\n                  <\/jats:p>","DOI":"10.1098\/rsob.210288","type":"journal-article","created":{"date-parts":[[2022,3,16]],"date-time":"2022-03-16T04:05:47Z","timestamp":1647403547000},"update-policy":"https:\/\/doi.org\/10.1098\/crossmark-policy","source":"Crossref","is-referenced-by-count":3,"title":["Improvement of the antibody-dependent respiratory burst assay for assessing protective immune responses to malaria"],"prefix":"10.1098","volume":"12","author":[{"given":"Annick","family":"Mansourou","sequence":"first","affiliation":[{"name":"Unit\u00e9 Immunologie, 36 Avenue Pasteur, 220 Dakar, S\u00e9n\u00e9gal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Charlotte","family":"Joos","sequence":"additional","affiliation":[{"name":"Unit\u00e9 Immunologie, 36 Avenue Pasteur, 220 Dakar, S\u00e9n\u00e9gal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Oumy","family":"Niass","sequence":"additional","affiliation":[{"name":"Unit\u00e9 Immunologie, 36 Avenue Pasteur, 220 Dakar, S\u00e9n\u00e9gal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Babacar","family":"Diouf","sequence":"additional","affiliation":[{"name":"Unit\u00e9 Immunologie, 36 Avenue Pasteur, 220 Dakar, S\u00e9n\u00e9gal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Adama","family":"Tall","sequence":"additional","affiliation":[{"name":"Unit\u00e9 Epid\u00e9miologie, 36 Avenue Pasteur, 220 Dakar, S\u00e9n\u00e9gal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ronald","family":"Perraut","sequence":"additional","affiliation":[{"name":"Unit\u00e9 Immunologie, 36 Avenue Pasteur, 220 Dakar, S\u00e9n\u00e9gal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0810-4595","authenticated-orcid":true,"given":"Makhtar","family":"Niang","sequence":"additional","affiliation":[{"name":"Unit\u00e9 Immunologie, 36 Avenue Pasteur, 220 Dakar, S\u00e9n\u00e9gal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aissatou","family":"Toure-Balde","sequence":"additional","affiliation":[{"name":"Unit\u00e9 Immunologie, 36 Avenue Pasteur, 220 Dakar, S\u00e9n\u00e9gal"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"175","published-online":{"date-parts":[[2022,3,16]]},"reference":[{"key":"e_1_3_6_2_2","volume-title":"World malaria report 2019","author":"WHO","year":"2020","unstructured":"WHO. 2020 World malaria report 2019. 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