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Here, through temporal application of Plk1 inhibitors, we identify a new role for Plk1 in the establishment of cortical polarity essential for highly asymmetric cell divisions of oocyte meiosis. Application of Plk1 inhibitors in late metaphase I abolishes pPlk1 from spindle poles and prevents the induction of actin polymerization at the cortex through inhibition of local recruitment of Cdc42 and Neuronal Wiskott-Aldrich Syndrome protein (N-WASP). By contrast, an already established polar actin cortex is insensitive to Plk1 inhibitors, but if the polar cortex is first depolymerized, Plk1 inhibitors completely prevent its restoration. Thus, Plk1 is essential for establishment but not maintenance of cortical actin polarity. These findings indicate that Plk1 regulates recruitment of Cdc42 and N-Wasp to coordinate cortical polarity and asymmetric cell division.<\/jats:p>","DOI":"10.1098\/rsob.220326","type":"journal-article","created":{"date-parts":[[2023,3,8]],"date-time":"2023-03-08T03:07:38Z","timestamp":1678244858000},"update-policy":"https:\/\/doi.org\/10.1098\/crossmark-policy","source":"Crossref","is-referenced-by-count":6,"title":["Polo-like kinase 1 promotes Cdc42-induced actin polymerization for asymmetric division in oocytes"],"prefix":"10.1098","volume":"13","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9968-3571","authenticated-orcid":true,"given":"Wai Shan","family":"Yuen","sequence":"first","affiliation":[{"name":"Department of Anatomy and Developmental Biology and Development and Stem Cells Program, Monash Biomedicine Discovery Institute, Monash University, Clayton, Victoria 3800, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qing Hua","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Anatomy and Developmental Biology and Development and Stem Cells Program, Monash Biomedicine Discovery Institute, Monash University, Clayton, Victoria 3800, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anne","family":"Bourdais","sequence":"additional","affiliation":[{"name":"University of Rennes, CNRS, IGDR - UMR 6290, F-35000 Rennes, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3404-8753","authenticated-orcid":false,"given":"Deepak","family":"Adhikari","sequence":"additional","affiliation":[{"name":"Department of Anatomy and Developmental Biology and Development and Stem Cells Program, Monash Biomedicine Discovery Institute, Monash University, Clayton, Victoria 3800, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6204-2105","authenticated-orcid":true,"given":"Guillaume","family":"Halet","sequence":"additional","affiliation":[{"name":"University of Rennes, CNRS, IGDR - UMR 6290, F-35000 Rennes, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9644-5861","authenticated-orcid":false,"given":"John","family":"Carroll","sequence":"additional","affiliation":[{"name":"Department of Anatomy and Developmental Biology and Development and Stem Cells Program, Monash Biomedicine Discovery Institute, Monash University, Clayton, Victoria 3800, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"175","published-online":{"date-parts":[[2023,3,8]]},"reference":[{"key":"e_1_3_7_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cub.2011.06.055"},{"key":"e_1_3_7_3_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrm3643"},{"key":"e_1_3_7_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/0012-1606(85)90320-3"},{"key":"e_1_3_7_5_2","first-page":"11","article-title":"Mechanism of polar body formation in the mouse oocyte: an interaction between the chromosomes, the cytoskeleton and the plasma membrane","volume":"92","author":"Maro B","year":"1986","unstructured":"Maro B, Johnson MH, Webb M, Flach G. 1986 Mechanism of polar body formation in the mouse oocyte: an interaction between the chromosomes, the cytoskeleton and the plasma membrane. 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