{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,16]],"date-time":"2026-04-16T07:33:02Z","timestamp":1776324782241,"version":"3.50.1"},"reference-count":99,"publisher":"The Royal Society","issue":"8","license":[{"start":{"date-parts":[[2023,8,1]],"date-time":"2023-08-01T00:00:00Z","timestamp":1690848000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/royalsociety.org\/journals\/ethics-policies\/data-sharing-mining\/"}],"funder":[{"name":"National Science Foundation","award":["MCB1818293"],"award-info":[{"award-number":["MCB1818293"]}]},{"name":"Minnesota Clinical and Translational Science Institute","award":["UL1TR002493"],"award-info":[{"award-number":["UL1TR002493"]}]},{"name":"Minnesota Clinical and Translational Science Institute","award":["UL1TR002494"],"award-info":[{"award-number":["UL1TR002494"]}]},{"name":"National Institute of Health","award":["5F30CA257227-02"],"award-info":[{"award-number":["5F30CA257227-02"]}]},{"name":"National Institute of Health","award":["R01GM074917"],"award-info":[{"award-number":["R01GM074917"]}]},{"name":"National Institute of Health","award":["R35GM141805"],"award-info":[{"award-number":["R35GM141805"]}]}],"content-domain":{"domain":["royalsocietypublishing.org"],"crossmark-restriction":true},"short-container-title":["Open Biol."],"published-print":{"date-parts":[[2023,8]]},"abstract":"<jats:p>\n                    DNA replication requires precise regulation achieved through post-translational modifications, including ubiquitination and SUMOylation. These modifications are linked by the SUMO-targeted E3 ubiquitin ligases (STUbLs). Ring finger protein 4 (RNF4), one of only two mammalian STUbLs, participates in double-strand break repair and resolving DNA\u2013protein cross-links. However, its role in DNA replication has been poorly understood. Using CRISPR\/Cas9 genetic screens, we discovered an unexpected dependency of\n                    <jats:italic>RNF4<\/jats:italic>\n                    mutants on\n                    <jats:italic>ubiquitin specific peptidase 7<\/jats:italic>\n                    (\n                    <jats:italic>USP7)<\/jats:italic>\n                    for survival in\n                    <jats:italic>TP53<\/jats:italic>\n                    -null retinal pigment epithelial cells.\n                    <jats:italic>\n                      TP53\n                      <jats:sup>\u2212\/\u2013<\/jats:sup>\n                      \/RNF4\n                      <jats:sup>\u2212\/\u2013<\/jats:sup>\n                      \/USP7\n                      <jats:sup>\u2212\/\u2013<\/jats:sup>\n                    <\/jats:italic>\n                    triple knockout (TKO) cells displayed defects in DNA replication that cause genomic instability. These defects were exacerbated by the proteasome inhibitor bortezomib, which limited the nuclear ubiquitin pool. A shortage of free ubiquitin suppressed the ataxia telangiectasia and Rad3-related (ATR)-mediated checkpoint response, leading to increased cell death. In conclusion, RNF4 and USP7 work cooperatively to sustain a functional level of nuclear ubiquitin to maintain the integrity of the genome.\n                  <\/jats:p>","DOI":"10.1098\/rsob.230068","type":"journal-article","created":{"date-parts":[[2023,8,22]],"date-time":"2023-08-22T19:06:48Z","timestamp":1692731208000},"update-policy":"https:\/\/doi.org\/10.1098\/crossmark-policy","source":"Crossref","is-referenced-by-count":8,"title":["RNF4 and USP7 cooperate in ubiquitin-regulated steps of DNA replication"],"prefix":"10.1098","volume":"13","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2058-3827","authenticated-orcid":true,"given":"Ya-Chu","family":"Chang","sequence":"first","affiliation":[{"name":"Department of Biochemistry, Molecular Biology and Biophysics, University of Minnesota, Minneapolis, MN 55455, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kevin","family":"Lin","sequence":"additional","affiliation":[{"name":"Department of Computer Science 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