{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,27]],"date-time":"2026-02-27T01:33:47Z","timestamp":1772156027154,"version":"3.50.1"},"reference-count":98,"publisher":"The Royal Society","issue":"9","license":[{"start":{"date-parts":[[2023,9,1]],"date-time":"2023-09-01T00:00:00Z","timestamp":1693526400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/royalsociety.org\/journals\/ethics-policies\/data-sharing-mining\/"}],"funder":[{"name":"MRC"},{"DOI":"10.13039\/100010269","name":"Wellcome Trust","doi-asserted-by":"crossref","id":[{"id":"10.13039\/100010269","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Max Planck Society"}],"content-domain":{"domain":["royalsocietypublishing.org"],"crossmark-restriction":true},"short-container-title":["Open Biol."],"published-print":{"date-parts":[[2023,9]]},"abstract":"<jats:p>\n                    Alterations in the neuromuscular system underlie several neuromuscular diseases and play critical roles in the development of sarcopenia, the age-related loss of muscle mass and function. Mammalian Myostatin (MST) and GDF11, members of the TGF-\u03b2 superfamily of growth factors, are powerful regulators of muscle size in both model organisms and humans. Myoglianin (MYO), the\n                    <jats:italic>Drosophila<\/jats:italic>\n                    homologue of MST and GDF11, is a strong inhibitor of synaptic function and structure at the neuromuscular junction in flies. Here, we identified Plum, a transmembrane cell surface protein, as a modulator of MYO function in the larval neuromuscular system. Reduction of Plum in the larval body-wall muscles abolishes the previously demonstrated positive effect of attenuated MYO signalling on both muscle size and neuromuscular junction structure and function. In addition, downregulation of\n                    <jats:italic>Plum<\/jats:italic>\n                    on its own results in decreased synaptic strength and body weight, classifying Plum as a (novel) regulator of neuromuscular function and body (muscle) size. These findings offer new insights into possible regulatory mechanisms behind ageing- and disease-related neuromuscular dysfunctions in humans and identify potential targets for therapeutic interventions.\n                  <\/jats:p>","DOI":"10.1098\/rsob.230171","type":"journal-article","created":{"date-parts":[[2023,9,12]],"date-time":"2023-09-12T19:05:43Z","timestamp":1694545543000},"update-policy":"https:\/\/doi.org\/10.1098\/crossmark-policy","source":"Crossref","is-referenced-by-count":3,"title":["Plum modulates Myoglianin and regulates synaptic function in\n                    <i>D. melanogaster<\/i>"],"prefix":"10.1098","volume":"13","author":[{"given":"Virender K.","family":"Sahota","sequence":"first","affiliation":[{"name":"Department of Biological Sciences, Centre for Biomedical Sciences, Royal Holloway University of London, Egham, Surrey TW20 0EX, UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Aelfwin","family":"Stone","sequence":"additional","affiliation":[{"name":"Faculty of Medicine &amp; Health Sciences, Queen's Medical Centre, Nottingham NG7 2UH, UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nathaniel S.","family":"Woodling","sequence":"additional","affiliation":[{"name":"Department of Biological Sciences, Centre for Biomedical Sciences, Royal Holloway University of London, Egham, Surrey TW20 0EX, UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jereme G.","family":"Spiers","sequence":"additional","affiliation":[{"name":"Faculty of Medicine &amp; Health Sciences, Queen's Medical Centre, Nottingham NG7 2UH, UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Joern R.","family":"Steinert","sequence":"additional","affiliation":[{"name":"Faculty of Medicine &amp; Health Sciences, Queen's Medical Centre, Nottingham NG7 2UH, UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Linda","family":"Partridge","sequence":"additional","affiliation":[{"name":"Institute of Healthy Ageing, and GEE, University College London, Darwin Building, Gower Street, London WC1E 6BT, UK"},{"name":"Max Planck Institute for Biology of Ageing, Joseph-Stelzmann-Str. 9b, Cologne 50931, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2041-3844","authenticated-orcid":true,"given":"Hrvoje","family":"Augustin","sequence":"additional","affiliation":[{"name":"Department of Biological Sciences, Centre for Biomedical Sciences, Royal Holloway University of London, Egham, Surrey TW20 0EX, UK"},{"name":"Institute of Healthy Ageing, and GEE, University College London, Darwin Building, Gower Street, London WC1E 6BT, UK"},{"name":"Max Planck Institute for Biology of Ageing, Joseph-Stelzmann-Str. 9b, Cologne 50931, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"175","published-online":{"date-parts":[[2023,9,13]]},"reference":[{"key":"e_1_3_8_2_2","doi-asserted-by":"publisher","DOI":"10.3389\/fnagi.2014.00208"},{"key":"e_1_3_8_3_2","doi-asserted-by":"publisher","DOI":"10.1093\/ageing\/afq034"},{"key":"e_1_3_8_4_2","doi-asserted-by":"publisher","DOI":"10.1177\/33.10.2931475"},{"key":"e_1_3_8_5_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature01135"},{"key":"e_1_3_8_6_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1475-097X.1985.tb00590.x"},{"key":"e_1_3_8_7_2","first-page":"1339","article-title":"Mechanisms of skeletal muscle aging: insights from Drosophila and mammalian models","volume":"6","author":"Demontis F","year":"2013","unstructured":"Demontis F, Piccirillo R, Goldberg AL, Perrimon N. 2013 Mechanisms of skeletal muscle aging: insights from Drosophila and mammalian models. Dis. Model Mech. 6, 1339-1352. (doi:10.1242\/dmm.012559)","journal-title":"Dis. Model Mech."},{"key":"e_1_3_8_8_2","first-page":"162","article-title":"Regulation of excitation contraction coupling by insulin-like growth factor-1 in aging skeletal muscle","volume":"4","author":"Delbono O","year":"2000","unstructured":"Delbono O. 2000 Regulation of excitation contraction coupling by insulin-like growth factor-1 in aging skeletal muscle. J. Nutr. Health Aging 4, 162-164.","journal-title":"J. Nutr. Health Aging"},{"key":"e_1_3_8_9_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF00235039"},{"key":"e_1_3_8_10_2","doi-asserted-by":"publisher","DOI":"10.1098\/rspb.1966.0008"},{"key":"e_1_3_8_11_2","doi-asserted-by":"publisher","DOI":"10.1002\/cne.22764"},{"key":"e_1_3_8_12_2","doi-asserted-by":"publisher","DOI":"10.1038\/srep27935"},{"key":"e_1_3_8_13_2","doi-asserted-by":"publisher","DOI":"10.1016\/0022-510X(84)90021-2"},{"key":"e_1_3_8_14_2","doi-asserted-by":"publisher","DOI":"10.1016\/0022-510X(90)90076-Y"},{"key":"e_1_3_8_15_2","doi-asserted-by":"publisher","DOI":"10.18632\/aging.101198"},{"key":"e_1_3_8_16_2","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.1983.sp014898"},{"key":"e_1_3_8_17_2","doi-asserted-by":"publisher","DOI":"10.1016\/0047-6374(73)90040-7"},{"key":"e_1_3_8_18_2","doi-asserted-by":"publisher","DOI":"10.3390\/ijms18102092"},{"key":"e_1_3_8_19_2","doi-asserted-by":"publisher","DOI":"10.1096\/fj.201700835R"},{"key":"e_1_3_8_20_2","doi-asserted-by":"publisher","DOI":"10.1007\/s12035-013-8578-4"},{"key":"e_1_3_8_21_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.clineuro.2016.08.020"},{"key":"e_1_3_8_22_2","doi-asserted-by":"publisher","DOI":"10.4081\/ejh.2012.e36"},{"key":"e_1_3_8_23_2","doi-asserted-by":"publisher","DOI":"10.3389\/fnagi.2015.00125"},{"key":"e_1_3_8_24_2","doi-asserted-by":"publisher","DOI":"10.3389\/fnagi.2014.00099"},{"key":"e_1_3_8_25_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.biocel.2013.05.001"},{"key":"e_1_3_8_26_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.182.4109.293"},{"key":"e_1_3_8_27_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1447-0594.2010.00608.x"},{"key":"e_1_3_8_28_2","doi-asserted-by":"publisher","DOI":"10.1007\/s004280000237"},{"key":"e_1_3_8_29_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbagen.2009.06.011"},{"key":"e_1_3_8_30_2","doi-asserted-by":"publisher","DOI":"10.1016\/bs.ctdb.2016.07.003"},{"key":"e_1_3_8_31_2","doi-asserted-by":"publisher","DOI":"10.3390\/molecules20046237"},{"key":"e_1_3_8_32_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0959-4388(96)80038-9"},{"key":"e_1_3_8_33_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0925-4773(99)00108-2"},{"key":"e_1_3_8_34_2","first-page":"2445","article-title":"Myostatin-like proteins regulate synaptic function and neuronal morphology","volume":"144","author":"Augustin H","year":"2017","unstructured":"Augustin H et al. 2017 Myostatin-like proteins regulate synaptic function and neuronal morphology. Development 144, 2445-2455. (doi:10.1242\/dev.152975)","journal-title":"Development"},{"key":"e_1_3_8_35_2","doi-asserted-by":"publisher","DOI":"10.2174\/138920207783591672"},{"key":"e_1_3_8_36_2","doi-asserted-by":"publisher","DOI":"10.1038\/387083a0"},{"key":"e_1_3_8_37_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuron.2013.03.004"},{"key":"e_1_3_8_38_2","doi-asserted-by":"publisher","DOI":"10.1006\/geno.2000.6114"},{"key":"e_1_3_8_39_2","doi-asserted-by":"publisher","DOI":"10.1186\/1471-213X-10-115"},{"key":"e_1_3_8_40_2","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.0473-10.2010"},{"key":"e_1_3_8_41_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1601-183X.2008.00425.x"},{"key":"e_1_3_8_42_2","first-page":"465","article-title":"Dscam and Sidekick proteins direct lamina-specific synaptic connections in the vertebrate lamina","volume":"451","author":"Yamagata M","year":"2008","unstructured":"Yamagata M, Sanes JR. 2008 Dscam and Sidekick proteins direct lamina-specific synaptic connections in the vertebrate lamina. Neuron 451, 465-469.","journal-title":"Neuron"},{"key":"e_1_3_8_43_2","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.25567"},{"key":"e_1_3_8_44_2","doi-asserted-by":"publisher","DOI":"10.1155\/2016\/7614971"},{"key":"e_1_3_8_45_2","doi-asserted-by":"publisher","DOI":"10.1038\/nn.2833"},{"key":"e_1_3_8_46_2","first-page":"D77","article-title":"Expression Atlas update: from tissues to single cells","volume":"48","author":"Papatheodorou I","year":"2020","unstructured":"Papatheodorou I et al. 2020 Expression Atlas update: from tissues to single cells. Nucleic Acids Res. 48, D77-D83. (doi:10.1093\/nar\/gkaa339)","journal-title":"Nucleic Acids Res."},{"key":"e_1_3_8_47_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0074-7742(06)75001-2"},{"key":"e_1_3_8_48_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cbpa.2009.04.626"},{"key":"e_1_3_8_49_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0896-6273(00)80415-8"},{"key":"e_1_3_8_50_2","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.19-08-03023.1999"},{"key":"e_1_3_8_51_2","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.4201-04.2005"},{"key":"e_1_3_8_52_2","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.4194-04.2005"},{"key":"e_1_3_8_53_2","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.22-17-07362.2002"},{"key":"e_1_3_8_54_2","doi-asserted-by":"publisher","DOI":"10.1038\/nn789"},{"key":"e_1_3_8_55_2","doi-asserted-by":"publisher","DOI":"10.4137\/BBI.S2010"},{"key":"e_1_3_8_56_2","doi-asserted-by":"publisher","DOI":"10.1038\/nn1484"},{"key":"e_1_3_8_57_2","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.1527-05.2005"},{"key":"e_1_3_8_58_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuron.2006.02.008"},{"key":"e_1_3_8_59_2","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.23-16-06546.2003"},{"key":"e_1_3_8_60_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0012778"},{"key":"e_1_3_8_61_2","doi-asserted-by":"publisher","DOI":"10.3389\/fnmol.2019.00232"},{"key":"e_1_3_8_62_2","doi-asserted-by":"crossref","first-page":"a022152","DOI":"10.1101\/cshperspect.a022152","article-title":"TGF-\u03b2 Family signalling in Drosophila","volume":"9","author":"Upadhyay A","year":"2017","unstructured":"Upadhyay A, Moss-Taylor L, Kim M-J, Ghosh AC, O'Connor MB. 2017 TGF-\u03b2 Family signalling in Drosophila. Cold Spring Harb. Prospect. Biol. 9, a022152.","journal-title":"Cold Spring Harb. Prospect. Biol."},{"key":"e_1_3_8_63_2","doi-asserted-by":"publisher","DOI":"10.1007\/s10495-019-01574-4"},{"key":"e_1_3_8_64_2","doi-asserted-by":"publisher","DOI":"10.1111\/asj.12052"},{"key":"e_1_3_8_65_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2005.12.060"},{"key":"e_1_3_8_66_2","doi-asserted-by":"publisher","DOI":"10.1152\/physrev.00033.2014"},{"key":"e_1_3_8_67_2","doi-asserted-by":"publisher","DOI":"10.1007\/s10522-015-9627-3"},{"key":"e_1_3_8_68_2","first-page":"471","article-title":"The relationship of peripheral motor nerve conduction velocity to age-associated loss of grip strength","volume":"10","author":"Metter EJ","year":"1998","unstructured":"Metter EJ, Conwit R, Metter B, Pacheco T, Tobin J. 1998 The relationship of peripheral motor nerve conduction velocity to age-associated loss of grip strength. Aging (Milano) 10, 471-478.","journal-title":"Aging (Milano)"},{"key":"e_1_3_8_69_2","doi-asserted-by":"publisher","DOI":"10.1152\/jn.00386.2014"},{"key":"e_1_3_8_70_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.neures.2006.02.002"},{"key":"e_1_3_8_71_2","doi-asserted-by":"publisher","DOI":"10.1093\/jn\/128.2.351S"},{"key":"e_1_3_8_72_2","doi-asserted-by":"publisher","DOI":"10.3389\/fphys.2015.00252"},{"key":"e_1_3_8_73_2","doi-asserted-by":"publisher","DOI":"10.1096\/fj.11-191262"},{"key":"e_1_3_8_74_2","doi-asserted-by":"publisher","DOI":"10.7600\/jpfsm.3.111"},{"key":"e_1_3_8_75_2","doi-asserted-by":"publisher","DOI":"10.3389\/fnana.2016.00006"},{"key":"e_1_3_8_76_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.exger.2012.03.002"},{"key":"e_1_3_8_77_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.exger.2012.05.021"},{"key":"e_1_3_8_78_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrm1074"},{"key":"e_1_3_8_79_2","doi-asserted-by":"publisher","DOI":"10.1016\/0896-6273(93)90153-I"},{"key":"e_1_3_8_80_2","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(94)90052-3"},{"key":"e_1_3_8_81_2","doi-asserted-by":"publisher","DOI":"10.1093\/emboj\/18.23.6762"},{"key":"e_1_3_8_82_2","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.3590-11.2011"},{"key":"e_1_3_8_83_2","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.4434-08.2009"},{"key":"e_1_3_8_84_2","doi-asserted-by":"publisher","DOI":"10.1097\/WCO.0b013e328364c079"},{"key":"e_1_3_8_85_2","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.17-21-08194.1997"},{"key":"e_1_3_8_86_2","doi-asserted-by":"publisher","DOI":"10.1093\/abbs\/gmx126"},{"key":"e_1_3_8_87_2","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.51710"},{"key":"e_1_3_8_88_2","doi-asserted-by":"publisher","DOI":"10.1093\/jb\/mvs089"},{"key":"e_1_3_8_89_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.mod.2010.04.001"},{"key":"e_1_3_8_90_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0107443"},{"key":"e_1_3_8_91_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1001695107"},{"key":"e_1_3_8_92_2","doi-asserted-by":"publisher","DOI":"10.1016\/0304-3940(94)90889-3"},{"key":"e_1_3_8_93_2","doi-asserted-by":"publisher","DOI":"10.1007\/978-3-319-27511-6_5"},{"key":"e_1_3_8_94_2","first-page":"559","article-title":"The transforming growth factor-betas: multifaceted regulators of the development and maintenance of skeletal muscles, motoneurons and Schwann cells","volume":"46","author":"McLennan IS","year":"2002","unstructured":"McLennan IS, Koishi K. 2002 The transforming growth factor-betas: multifaceted regulators of the development and maintenance of skeletal muscles, motoneurons and Schwann cells. Int. J. Dev. Biol. 46, 559-567.","journal-title":"Int. J. Dev. Biol."},{"key":"e_1_3_8_95_2","doi-asserted-by":"publisher","DOI":"10.1093\/gerona\/62.10.1071"},{"key":"e_1_3_8_96_2","doi-asserted-by":"publisher","DOI":"10.1242\/dev.118.2.401"},{"key":"e_1_3_8_97_2","doi-asserted-by":"publisher","DOI":"10.1093\/hmg\/ddu171"},{"key":"e_1_3_8_98_2","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.2006.123612"},{"key":"e_1_3_8_99_2","unstructured":"Sahota VK Stone A Woodling NS Spiers JG Steinert JR Partridge L Augustin H. 2023 Plum modulates myoglianin and regulates synaptic function in D. melanogaster . Figshare. (doi:10.6084\/m9.figshare.c.6812307)"}],"container-title":["Open Biology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/royalsocietypublishing.org\/doi\/pdf\/10.1098\/rsob.230171","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/royalsocietypublishing.org\/doi\/full-xml\/10.1098\/rsob.230171","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/royalsocietypublishing.org\/doi\/pdf\/10.1098\/rsob.230171","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,12]],"date-time":"2023-09-12T19:05:59Z","timestamp":1694545559000},"score":1,"resource":{"primary":{"URL":"https:\/\/royalsocietypublishing.org\/doi\/10.1098\/rsob.230171"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,9]]},"references-count":98,"journal-issue":{"issue":"9","published-print":{"date-parts":[[2023,9]]}},"alternative-id":["10.1098\/rsob.230171"],"URL":"https:\/\/doi.org\/10.1098\/rsob.230171","relation":{"has-review":[{"id-type":"doi","id":"10.1098\/RSOB.230171\/v1\/decision1","asserted-by":"object"},{"id-type":"doi","id":"10.1098\/RSOB.230171\/v2\/response1","asserted-by":"object"},{"id-type":"doi","id":"10.1098\/RSOB.230171\/v1\/review1","asserted-by":"object"},{"id-type":"doi","id":"10.1098\/RSOB.230171\/v2\/decision1","asserted-by":"object"}]},"ISSN":["2046-2441"],"issn-type":[{"value":"2046-2441","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,9]]},"assertion":[{"value":"2023-06-03","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-08-14","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-09-13","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"230171"}}