{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,21]],"date-time":"2026-09-21T17:49:21Z","timestamp":1790012961987,"version":"4.0.1"},"reference-count":99,"publisher":"The Royal Society","issue":"6","license":[{"start":{"date-parts":[[2024,6,1]],"date-time":"2024-06-01T00:00:00Z","timestamp":1717200000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2024,6,1]],"date-time":"2024-06-01T00:00:00Z","timestamp":1717200000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"State Agency of Research, Spanish State"},{"DOI":"10.13039\/501100004281","name":"National Science Centre of Poland","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100004281","id-type":"DOI","asserted-by":"crossref"}]},{"name":"National Government of Catalonia"}],"content-domain":{"domain":["royalsocietypublishing.org"],"crossmark-restriction":true},"short-container-title":["Open Biol."],"published-print":{"date-parts":[[2024,6]]},"abstract":"<jats:p>\n                    Gram-negative bacteria from the Bacteroidota phylum possess a type-IX secretion system (T9SS) for protein secretion, which requires cargoes to have a C-terminal domain (CTD). Structurally analysed CTDs are from\n                    <jats:italic>Porphyromonas gingivalis<\/jats:italic>\n                    proteins RgpB, HBP35, PorU and PorZ, which share a compact immunoglobulin-like antiparallel 3+4 \u03b2-sandwich (\u03b21\u2013\u03b27). This architecture is essential as a\n                    <jats:italic>P. gingivalis<\/jats:italic>\n                    strain with a single-point mutant of RgpB disrupting the interaction of the CTD with its preceding domain prevented secretion of the protein. Next, we identified the C-terminus (\u2018motif C-t.\u2019) and the loop connecting strands \u03b23 and \u03b24 (\u2018motif L\u03b23\u03b24\u2019) as conserved. We generated two strains with insertion and replacement mutants of PorU, as well as three strains with ablation and point mutants of RgpB, which revealed both motifs to be relevant for T9SS function. Furthermore, we determined the crystal structure of the CTD of mirolase, a cargo of the\n                    <jats:italic>Tannerella forsythia<\/jats:italic>\n                    T9SS\n                    <jats:italic>,<\/jats:italic>\n                    which shares the same general topology as in\n                    <jats:italic>Porphyromonas<\/jats:italic>\n                    CTDs. However, motif L\u03b23\u03b24 was not conserved. Consistently,\n                    <jats:italic>P. gingivalis<\/jats:italic>\n                    could not properly secrete a chimaeric protein with the CTD of peptidylarginine deiminase replaced with this foreign CTD. Thus, the incompatibility of the CTDs between these species prevents potential interference between their T9SSs.\n                  <\/jats:p>","DOI":"10.1098\/rsob.230448","type":"journal-article","created":{"date-parts":[[2024,6,11]],"date-time":"2024-06-11T19:05:15Z","timestamp":1718132715000},"update-policy":"https:\/\/doi.org\/10.1098\/crossmark-policy","source":"Crossref","is-referenced-by-count":11,"title":["Structural and functional insights into the C-terminal signal domain of the Bacteroidetes type-IX secretion system"],"prefix":"10.1098","volume":"14","author":[{"given":"Danuta","family":"Mizgalska","sequence":"first","affiliation":[{"name":"Department of Microbiology, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Krak\u00f3w, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Arturo","family":"Rodr\u00edguez-Banqueri","sequence":"additional","affiliation":[{"name":"Proteolysis Laboratory, Department of Structural Biology, Molecular Biology Institute of Barcelona (CSIC), Barcelona, Catalonia 08028, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Florian","family":"Veillard","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Krak\u00f3w, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Miros\u0142aw","family":"Ksi\u0105\u017c\u0119k","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Krak\u00f3w, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Theodoros","family":"Goulas","sequence":"additional","affiliation":[{"name":"Department of Food Science and Nutrition, School of Agricultural Sciences, University of Thessaly, Karditsa 43100, Greece"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tibisay","family":"Guevara","sequence":"additional","affiliation":[{"name":"Proteolysis Laboratory, Department of Structural Biology, Molecular Biology Institute of Barcelona (CSIC), Barcelona, Catalonia 08028, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5863-4514","authenticated-orcid":true,"given":"Ulrich","family":"Eckhard","sequence":"additional","affiliation":[{"name":"Synthetic Structural Biology Group, Department of Structural Biology, Molecular Biology Institute of Barcelona (CSIC), Barcelona, Catalonia 08028, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jan","family":"Potempa","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Krak\u00f3w, Poland"},{"name":"Department of Oral Immunology and Infectious Diseases, University of Louisville School of Dentistry, Louisville, KY 40202, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6848-6874","authenticated-orcid":true,"given":"F. 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