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Fatty acids are critical for the growth and proliferation of\n                    <jats:italic>T. gondii<\/jats:italic>\n                    , which has at least two pathways to synthesize fatty acids, including the type II de novo synthesis pathway in the apicoplast and the elongation pathway in the endoplasmic reticulum (ER). Acetyl-CoA is the key substrate for both fatty acid synthesis pathways. In the apicoplast, acetyl-CoA is mainly provided by the pyruvate dehydrogenase complex. However, how the ER acquires acetyl-CoA is not fully understood. Here, we identified a putative acetyl-CoA transporter (TgAT1) that localized to the ER of\n                    <jats:italic>T. gondii<\/jats:italic>\n                    . Deletion of TgAT1 impaired parasite growth and invasion\n                    <jats:italic>in vitro<\/jats:italic>\n                    and attenuated tachyzoite virulence\n                    <jats:italic>in vivo<\/jats:italic>\n                    . Metabolic tracing using\n                    <jats:sup>13<\/jats:sup>\n                    C-acetate found that loss of TgAT1 reduced the incorporation of\n                    <jats:sup>13<\/jats:sup>\n                    C into certain fatty acids, suggesting reduced activities of elongation. Truncation of AT1 was previously reported to confer resistance to the antimalarial compound GNF179 in\n                    <jats:italic>Plasmodium falciparum<\/jats:italic>\n                    . Interestingly, GNF179 had much weaker inhibitory effect on\n                    <jats:italic>Toxoplasma<\/jats:italic>\n                    than on\n                    <jats:italic>Plasmodium<\/jats:italic>\n                    . In addition, deletion of AT1 did not affect the susceptibility of\n                    <jats:italic>Toxoplasma<\/jats:italic>\n                    to GNF179, suggesting that this compound might be taken up differently or has different inhibitory mechanisms in these parasites. Together, our data show that TgAT1 has important roles for parasite growth and fatty acid synthesis, but its disruption does not confer GNF179 resistance in\n                    <jats:italic>T. gondii<\/jats:italic>\n                    .\n                  <\/jats:p>","DOI":"10.1098\/rsob.240184","type":"journal-article","created":{"date-parts":[[2024,11,12]],"date-time":"2024-11-12T19:05:17Z","timestamp":1731438317000},"update-policy":"https:\/\/doi.org\/10.1098\/crossmark-policy","source":"Crossref","is-referenced-by-count":5,"title":["An endoplasmic reticulum localized acetyl-CoA transporter is required for efficient fatty acid synthesis in\n                    <i>Toxoplasma gondii<\/i>"],"prefix":"10.1098","volume":"14","author":[{"given":"Biyun","family":"Qin","sequence":"first","affiliation":[{"name":"National Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, Hubei Province, People\u2019s Republic of 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