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They cluster as a triplet of genes on the X chromosome, but their function remains unknown. Here, we demonstrate that\n                    <jats:italic>Rtl8a<\/jats:italic>\n                    and\n                    <jats:italic>Rtl8b<\/jats:italic>\n                    play important roles in the brain: their double knockout (DKO) mice not only exhibit reduced social responses and increased apathy-like behaviour, but also become obese from young adulthood, similar to patients with late Prader\u2013Willi syndrome (PWS), a neurodevelopmental genomic imprinting disorder. Mouse RTL8A\/8B proteins are expressed in the prefrontal cortex and hypothalamus and localize to both the nucleus and cytoplasm of neurons, presumably due to the N-terminal nuclear localization signal-like sequence at the N-terminus. An RNAseq study in the cerebral cortex revealed reduced expression of several GABA type A receptor subunit genes in DKO, in particular\n                    <jats:italic>Gabrb2,<\/jats:italic>\n                    which encodes its \u03b22 subunit. We confirmed the reduction of GABRB2 protein in the DKO cerebral cortex by western blotting. As GABRB2 has been implicated in the aetiology of several neurodevelopmental and neuropsychiatric disorders, it is likely that the reduction of GABRB2 is one of the major causes of the neuropsychiatric defects in the DKO mice.\n                  <\/jats:p>","DOI":"10.1098\/rsob.240279","type":"journal-article","created":{"date-parts":[[2025,1,28]],"date-time":"2025-01-28T19:07:24Z","timestamp":1738091244000},"update-policy":"https:\/\/doi.org\/10.1098\/crossmark-policy","source":"Crossref","is-referenced-by-count":4,"title":["Targeting of retrovirus-derived\n                    <i>Rtl8a<\/i>\n                    \/\n                    <i>8b<\/i>\n                    causes late-onset obesity, reduced social response and increased apathy-like behaviour"],"prefix":"10.1098","volume":"15","author":[{"given":"Yoshifumi","family":"Fujioka","sequence":"first","affiliation":[{"name":"Department of Epigenetics, Medical Research Institute (MRI), Tokyo Medical and Dental University (TMDU), 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