{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,4]],"date-time":"2026-08-04T03:06:32Z","timestamp":1785812792736,"version":"3.56.0"},"reference-count":35,"publisher":"The Royal Society","issue":"6","license":[{"start":{"date-parts":[[2025,6,1]],"date-time":"2025-06-01T00:00:00Z","timestamp":1748736000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,6,1]],"date-time":"2025-06-01T00:00:00Z","timestamp":1748736000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100011889","name":"Diamond Light Source","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100011889","id-type":"DOI","asserted-by":"publisher"}]},{"name":"NSF XSEDE"},{"DOI":"10.13039\/501100000268","name":"BBSRC","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100000268","id-type":"DOI","asserted-by":"crossref"}]},{"name":"EPSRC Centre for Doctoral Training in Fluid Dynamics"},{"name":"Glasgow Children\u2019s Hospital Charity"},{"DOI":"10.13039\/501100001671","name":"European Synchrotron Radiation Facility","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001671","id-type":"DOI","asserted-by":"publisher"}]},{"name":"NSF"}],"content-domain":{"domain":["royalsocietypublishing.org"],"crossmark-restriction":true},"short-container-title":["Open Biol."],"published-print":{"date-parts":[[2025,6]]},"abstract":"<jats:p>\n                    Enterohaemorrhagic\n                    <jats:italic toggle=\"yes\">Escherichia coli<\/jats:italic>\n                    causes sporadic, and sometimes large-scale, food poisoning outbreaks, for which antibiotic treatment in humans is contraindicated. As an alternative form of therapy, previous studies developed the family of salicylidene acylhydrazide (SA) anti-virulence compounds. One target of the SA compounds is AdhE, an enzyme that converts acetyl-CoA to ethanol and vice versa. AdhE oligomerizes, forming helicoidal filaments, heterogeneous in length, called spirosomes. We show it is possible to only partially fractionate AdhE spirosomes because\n                    <jats:italic toggle=\"yes\">in vitro<\/jats:italic>\n                    they oligomerize in the absence of stimuli, and that spirosome formation is necessary to regulate the direction of AdhE enzymatic reactions. We also show that the SA compound ME0054 binds and perturbs AdhE spirosomes, enhancing the conversion of ethanol to acetyl-CoA. This mechanistic understanding of how ME0054 impacts AdhE function will help in the development of SA compounds as novel anti-virulence inhibitors.\n                  <\/jats:p>","DOI":"10.1098\/rsob.250041","type":"journal-article","created":{"date-parts":[[2025,6,17]],"date-time":"2025-06-17T19:06:03Z","timestamp":1750187163000},"update-policy":"https:\/\/doi.org\/10.1098\/crossmark-policy","source":"Crossref","is-referenced-by-count":2,"title":["Enterohaemorrhagic\n                    <i>Escherichia coli<\/i>\n                    AdhE spirosome length correlates with enzymatic directionality and is perturbed by salicylidene acylhydrazides"],"prefix":"10.1098","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5075-2954","authenticated-orcid":true,"given":"Ester","family":"Serrano","sequence":"first","affiliation":[{"name":"School of Infection and Immunity, University of Glasgow, Glasgow G12 8TA, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8078-0638","authenticated-orcid":true,"given":"Tianxiao","family":"Zhao","sequence":"additional","affiliation":[{"name":"EaStCHEM School of Chemistry, University of Edinburgh, Edinburgh EH9 3FJ, UK"},{"name":"IRR Chemistry Hub, Institute for Regeneration and Repair, University of Edinburgh, Edinburgh EH16 4UU, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1688-5224","authenticated-orcid":true,"given":"David R.","family":"Mark","sequence":"additional","affiliation":[{"name":"School of Infection and Immunity, University of Glasgow, Glasgow G12 8TA, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1112-8387","authenticated-orcid":true,"given":"Mostafa","family":"Soroor","sequence":"additional","affiliation":[{"name":"EPSRC CDT in Fluid Dynamics, School of Computing, University of Leeds, Leeds LS2 9JT, UK"},{"name":"Diamond Light Source, Harwell Science and Innovation Campus, Didcot OX11 0DE, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0007-9251-527X","authenticated-orcid":true,"given":"Iris","family":"Floria","sequence":"additional","affiliation":[{"name":"School of Infection and Immunity, University of Glasgow, Glasgow G12 8TA, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8783-1282","authenticated-orcid":true,"given":"Nicholas J.","family":"Terrill","sequence":"additional","affiliation":[{"name":"Diamond Light Source, Harwell Science and Innovation Campus, Didcot OX11 0DE, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1041-8390","authenticated-orcid":true,"given":"Nikil","family":"Kapur","sequence":"additional","affiliation":[{"name":"School of Mechanical Engineering, University of Leeds, Leeds LS2 9JT, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2116-1084","authenticated-orcid":true,"given":"Arwen I. 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