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However, the study of intestinal mucosal immunity in teleosts has been hindered by the lack of reliable and specific marker genes to distinguish between different cell types. In this study, single-cell RNA-seq analysis of the intestinal epithelial cells from healthy and infected Sebastes schlegeli was performed to investigate the heterogeneity of teleost intestinal epithelial cells. Immune cells, including T cells, B cells, non-specific cytotoxic cells (NCCs) and myeloid cells, and non-immune cells including enterocyte, erythrocyte, BEST4+ cell, goblet cell and enteroendocrine cell, were identified. Several notable cell populations, including early myeloid cells, lysosome-rich enterocytes, enterocyte progenitors and BEST4+ cells, were characterized in detail. Additionally, significant changes in the expression of actin polymerization-related genes were observed in various intestinal mucosal cell types following Edwardsiella piscicida infection, suggesting a mechanism of invasion with E. piscicida. These findings enhance our understanding of teleost intestinal cellular composition and provide valuable insights for the development of therapeutic strategies against E. piscicida.<\/jats:p>","DOI":"10.1098\/rsob.250346","type":"journal-article","created":{"date-parts":[[2026,8,5]],"date-time":"2026-08-05T09:17:26Z","timestamp":1785921446000},"source":"Crossref","is-referenced-by-count":0,"title":["Regulation of actin polarization by\n                    <i>Edwardsiella piscicida<\/i>\n                    during invasion of\n                    <i>Sebastes schlegeli<\/i>\n                    intestinal mucosa and cellular immune responses"],"prefix":"10.1098","volume":"16","author":[{"given":"Ting","family":"Xue","sequence":"first","affiliation":[{"name":"School of Marine Science and Engineering, Qingdao Agricultural University 1 , Qingdao, People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yanmin","family":"Wang","sequence":"additional","affiliation":[{"name":"School of Marine Science and Engineering, Qingdao Agricultural University 1 , Qingdao, People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Baofeng","family":"Su","sequence":"additional","affiliation":[{"name":"School of Fisheries, Aquaculture and Aquatic Sciences, Auburn University 2 , Auburn, AL, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Min","family":"Cao","sequence":"additional","affiliation":[{"name":"School of Marine Science and Engineering, Qingdao Agricultural University 1 , Qingdao, People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuping","family":"Liu","sequence":"additional","affiliation":[{"name":"School of Marine Science and Engineering, Qingdao Agricultural University 1 , Qingdao, People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yiping","family":"Liu","sequence":"additional","affiliation":[{"name":"School of Marine Science and Engineering, Qingdao Agricultural University 1 , Qingdao, People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1950-0190","authenticated-orcid":false,"given":"Chao","family":"Li","sequence":"additional","affiliation":[{"name":"School of Marine Science and Engineering, Qingdao Agricultural University 1 , Qingdao, People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"175","published-online":{"date-parts":[[2026,8,5]]},"reference":[{"key":"2026080505172288500_RSOB250346C1","doi-asserted-by":"crossref","first-page":"108572","DOI":"10.1016\/j.fsi.2023.108572","article-title":"Immunity of the intestinal mucosa in teleost fish","volume":"133","author":"Stosik","year":"2023","journal-title":"Fish. 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