{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,22]],"date-time":"2026-10-22T18:58:24Z","timestamp":1792695504120,"version":"4.5.0"},"reference-count":56,"publisher":"The Royal Society","issue":"5","license":[{"start":{"date-parts":[[2026,5,27]],"date-time":"2026-05-27T00:00:00Z","timestamp":1779840000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,5,27]],"date-time":"2026-05-27T00:00:00Z","timestamp":1779840000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100004901","name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado de Minas Gerais","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100004901","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003593","name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002322","name":"Coordena\u00e7\u00e3o de Aperfei\u00e7oamento de Pessoal de N\u00edvel Superior","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100002322","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,5,27]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Aeromonas species are globally significant pathogens. However, the mechanisms driving their antimicrobial resistance patterns remain unclear. This study addresses the spread of resistance genes in the Aeromonas genus through a large-scale genomic analysis of all complete Aeromonas genomes in the RefSeq database. The emergence of next-generation genomic sequencing enabled the sequencing, assembling and annotation of numerous genomes with a description and characterization of the genomic plasticity and the pan-resistome, through bioinformatics programmes, of each species in the Aeromonas genus, and revealed species-specific patterns of resistance determinants. Leveraging these genomic insights, we applied a reverse vaccinology approach with a subtractive genomic workflow to select novel in silico vaccine targets for the three main pathogens: A. veronii, A. hydrophila and A. caviae. These protein candidates offer a potential alternative to prevent the spread of antibiotic resistance genes. Our findings underscore that continuous genomic surveillance is essential for monitoring established and emerging pathogens. While further in vitro and in vivo validation is pending, this work provides a robust framework for understanding Aeromonas resistance and developing new strategies to protect public and environmental health.<\/jats:p>","DOI":"10.1098\/rsob.250423","type":"journal-article","created":{"date-parts":[[2026,5,27]],"date-time":"2026-05-27T00:06:36Z","timestamp":1779840396000},"source":"Crossref","is-referenced-by-count":1,"title":["Pan-resistome and genomic plasticity analysis of antibiotic resistance in\n                    <i>Aeromonas:<\/i>\n                    new vaccine targets for\n                    <i>A. caviae, A. veronii<\/i>\n                    and\n                    <i>A. hydrophila<\/i>\n                    through reverse vaccinology"],"prefix":"10.1098","volume":"16","author":[{"ORCID":"https:\/\/orcid.org\/0009-0004-9660-0816","authenticated-orcid":false,"given":"Caio Luigi Antunes Moura","family":"Trist\u00e3o","sequence":"first","affiliation":[{"name":"UFTM 1 , , ,","place":["Uberaba, Minas Gerais, Brazil"],"department":["Department of Microbiology, Immunology and Parasitology"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrei Giacchetto","family":"Felice","sequence":"additional","affiliation":[{"name":"UFTM 1 , , ,","place":["Uberaba, Minas Gerais, Brazil"],"department":["Department of Microbiology, Immunology and Parasitology"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ligia Carolina da Silva","family":"Prado","sequence":"additional","affiliation":[{"name":"UFTM 1 , , ,","place":["Uberaba, Minas Gerais, Brazil"],"department":["Department of Microbiology, Immunology and Parasitology"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mateus","family":"Matiuzzi da Costa","sequence":"additional","affiliation":[{"name":"UNIVASF 2 , ,","place":["Petrolina, Brazil"],"department":["Center for Open Access Genomic Analysis (CALAnGO)"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gisele","family":"Veneroni Gouveia","sequence":"additional","affiliation":[{"name":"UNIVASF 2 , ,","place":["Petrolina, Brazil"],"department":["Center for Open Access Genomic Analysis (CALAnGO)"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pedro Henrique","family":"Marques","sequence":"additional","affiliation":[{"name":"UFTM 1 , , ,","place":["Uberaba, Minas Gerais, Brazil"],"department":["Department of Microbiology, Immunology and Parasitology"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7299-3724","authenticated-orcid":false,"given":"Siomar de Castro","family":"Soares","sequence":"additional","affiliation":[{"name":"UFTM 1 , , ,","place":["Uberaba, Minas Gerais, Brazil"],"department":["Department of Microbiology, Immunology and Parasitology"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"175","published-online":{"date-parts":[[2026,5,27]]},"reference":[{"key":"2026060109361396100_R1","doi-asserted-by":"crossref","DOI":"10.3389\/fmicb.2017.00931","article-title":"Species distribution and prevalence of putative virulence factors in mesophilic Aeromonas spp. isolated from fresh retail sushi","volume":"8","author":"Hoel","year":"2017","journal-title":"Front. 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