{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T00:51:51Z","timestamp":1791334311447,"version":"4.3.1"},"reference-count":79,"publisher":"The Royal Society","issue":"10","license":[{"start":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T00:00:00Z","timestamp":1791331200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001824","name":"Czech Science Foundation","doi-asserted-by":"publisher","award":["21-03305S"],"award-info":[{"award-number":["21-03305S"]}],"id":[{"id":"10.13039\/501100001824","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001824","name":"Czech Science Foundation","doi-asserted-by":"publisher","award":["23-07532S"],"award-info":[{"award-number":["23-07532S"]}],"id":[{"id":"10.13039\/501100001824","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001824","name":"Czech Science Foundation","doi-asserted-by":"publisher","award":["25-18241S"],"award-info":[{"award-number":["25-18241S"]}],"id":[{"id":"10.13039\/501100001824","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Grant Agency of the University of South Bohemia","award":["GAJU, 081\/2020\/P"],"award-info":[{"award-number":["GAJU, 081\/2020\/P"]}]},{"name":"Research Programme Strategy AV21, \u2018Future of Assisted Reproduction\u2019"},{"name":"GA\u010cR Junior Star","award":["24-11761M."],"award-info":[{"award-number":["24-11761M."]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,10,7]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>In mammalian preimplantation embryos, distinct cell lineages occupy defined spatial niches. The outer trophectoderm (TE) forms an epithelial monolayer surrounding the inner cell mass (ICM) and blastocyst cavity. In mice, the Tead4 gene encodes a polarity-dependent transcription factor required for TE specification. Here, we demonstrate that Tead4 also preserves TE integrity during blastocyst maturation. Clonal siRNA-mediated Tead4 knockdown (KD) disrupts outer cell apical domain morphology, causing abnormal allocation of these clones to an enlarged ICM, despite minimal impact on established polarity. Fixed sample and live embryo light-sheet microscopy imaging reveal that TEAD4-deficient outer cells atypically migrate into the blastocyst ICM, sometimes via apical domain abscission, or are repositioned post-division, linking disrupted apical domain morphology to altered spatial positioning and fate. Transcriptomic analysis indicates that TEAD4 regulates genes involved in cytoskeletal organization, particularly those related to actin and cell adhesion, which we propose are critical for maintaining outer cell TE positioning. Combined KD of Tead4 and two of its targets, the atypical GTPases Rnd1 and Rnd3, partially rescues misallocation but does not prevent apical morphological abnormalities. These findings indicate TEAD4 and its downstream regulatory network actively contribute to blastocyst TE maintenance, beyond initial specification, up until the peri-implantation stage, ensuring proper lineage segregation.<\/jats:p>","DOI":"10.1098\/rsob.250441","type":"journal-article","created":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T00:10:42Z","timestamp":1791331842000},"source":"Crossref","is-referenced-by-count":0,"title":["TEAD4 Regulates apical domain homeostasis and cell-positioning to maintain the trophectoderm lineage during mouse blastocyst development"],"prefix":"10.1098","volume":"16","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6604-4845","authenticated-orcid":false,"given":"Rebecca","family":"Collier","sequence":"first","affiliation":[{"name":"Department of Molecular Biology and Genetics (Faculty of Science), University of South Bohemia in Ceske Budejovice 1 , Ceske Budejovice, South Bohemian Region, Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2015-7190","authenticated-orcid":false,"given":"Martina","family":"Bohuslavov\u00e1","sequence":"additional","affiliation":[{"name":"Department of Molecular Biology and Genetics (Faculty of Science), University of South Bohemia in Ceske Budejovice 1 , Ceske Budejovice, South Bohemian Region, Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4928-5732","authenticated-orcid":false,"given":"Michaela","family":"Va\u0161kovi\u010dov\u00e1","sequence":"additional","affiliation":[{"name":"Institute of Animal Physiology and Genetics CAS 2 , Lib\u011bchov, Central Bohemian Region, Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9420-7122","authenticated-orcid":false,"given":"Aleksandar I.","family":"Mihajlovi\u0107","sequence":"additional","affiliation":[{"name":"Department of Molecular Biology and Genetics (Faculty of Science), University of South Bohemia in Ceske Budejovice 1 , Ceske Budejovice, South Bohemian Region, Czech Republic"},{"name":"Centre de Recherche du Centre Hospitalier de l'Universite de Montreal 3 , Montreal, Quebec, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6277-9713","authenticated-orcid":false,"given":"Andrea","family":"Hauserov\u00e1","sequence":"additional","affiliation":[{"name":"Department of Molecular Biology and Genetics (Faculty of Science), University of South Bohemia in Ceske Budejovice 1 , Ceske Budejovice, South Bohemian Region, Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4228-0413","authenticated-orcid":false,"given":"Valeriya","family":"Zabelina","sequence":"additional","affiliation":[{"name":"Department of Molecular Biology and Genetics (Faculty of Science), University of South Bohemia in Ceske Budejovice 1 , Ceske Budejovice, South Bohemian Region, Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0948-1045","authenticated-orcid":false,"given":"Monika","family":"Fluks","sequence":"additional","affiliation":[{"name":"Department of Molecular Biology and Genetics (Faculty of Science), University of South Bohemia in Ceske Budejovice 1 , Ceske Budejovice, South Bohemian Region, Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9412-7971","authenticated-orcid":false,"given":"Lenka","family":"Gahurov\u00e1","sequence":"additional","affiliation":[{"name":"Department of Molecular Biology and Genetics (Faculty of Science), University of South Bohemia in Ceske Budejovice 1 , Ceske Budejovice, South Bohemian Region, Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7099-3042","authenticated-orcid":false,"given":"D\u00e1vid","family":"Drutovi\u010d","sequence":"additional","affiliation":[{"name":"Institute of Animal Physiology and Genetics CAS 2 , Lib\u011bchov, Central Bohemian Region, Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4297-4412","authenticated-orcid":false,"given":"Alexander W.","family":"Bruce","sequence":"additional","affiliation":[{"name":"Department of Molecular Biology and Genetics (Faculty of Science), University of South Bohemia in Ceske Budejovice 1 , Ceske Budejovice, South Bohemian Region, Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"175","published-online":{"date-parts":[[2026,10,7]]},"reference":[{"key":"2026100620100339400_RSOB250441C1","doi-asserted-by":"crossref","first-page":"1063","DOI":"10.1242\/dev.128314","article-title":"Lineage specification in the mouse preimplantation embryo","volume":"143","author":"Chazaud","year":"2016","journal-title":"Development"},{"key":"2026100620100339400_RSOB250441C2","doi-asserted-by":"crossref","first-page":"173","DOI":"10.1016\/bs.ctdb.2016.04.006","article-title":"The genetic regulation of cell fate during preimplantation mouse development","volume":"120","author":"Lokken","year":"2016","journal-title":"Curr. Top Dev. Biol."},{"key":"2026100620100339400_RSOB250441C3","doi-asserted-by":"crossref","first-page":"dev183079","DOI":"10.1242\/dev.183079","article-title":"Common principles of early mammalian embryo self-organisation","volume":"147","author":"P\u0142usa","year":"2020","journal-title":"Development"},{"key":"2026100620100339400_RSOB250441C4","doi-asserted-by":"crossref","first-page":"467","DOI":"10.1038\/nrg2564","article-title":"Making a firm decision: multifaceted regulation of cell fate in the early mouse embryo","volume":"10","author":"Zernicka-Goetz","year":"2009","journal-title":"Nat. Rev. Genet."},{"key":"2026100620100339400_RSOB250441C5","doi-asserted-by":"crossref","first-page":"352","DOI":"10.1016\/0012-1606(82)90384-0","article-title":"Allocation of cells to inner cell mass and trophectoderm lineages in preimplantation mouse embryos","volume":"90","author":"Balakier","year":"1982","journal-title":"Dev. Biol."},{"key":"2026100620100339400_RSOB250441C6","doi-asserted-by":"crossref","first-page":"520","DOI":"10.1016\/0012-1606(87)90055-8","article-title":"A quantitative analysis of cell allocation to trophectoderm and inner cell mass in the mouse blastocyst","volume":"119","author":"Fleming","year":"1987","journal-title":"Dev. Biol."},{"key":"2026100620100339400_RSOB250441C7","doi-asserted-by":"crossref","first-page":"6364","DOI":"10.1073\/pnas.0915063107","article-title":"Origin and formation of the first two distinct cell types of the inner cell mass in the mouse embryo","volume":"107","author":"Morris","year":"2010","journal-title":"Proc. Natl Acad. Sci. USA"},{"key":"2026100620100339400_RSOB250441C8","doi-asserted-by":"crossref","first-page":"615","DOI":"10.1016\/j.devcel.2006.02.020","article-title":"Early lineage segregation between epiblast and primitive endoderm in mouse blastocysts through the Grb2-MAPK pathway","volume":"10","author":"Chazaud","year":"2006","journal-title":"Dev. Cell"},{"key":"2026100620100339400_RSOB250441C9","doi-asserted-by":"crossref","first-page":"210","DOI":"10.1016\/j.ydbio.2009.04.036","article-title":"Active cell movements coupled to positional induction are involved in lineage segregation in the mouse blastocyst","volume":"331","author":"Meilhac","year":"2009","journal-title":"Dev. Biol."},{"key":"2026100620100339400_RSOB250441C10","doi-asserted-by":"crossref","first-page":"3081","DOI":"10.1242\/dev.021519","article-title":"Distinct sequential cell behaviours direct primitive endoderm formation in the mouse blastocyst","volume":"135","author":"Plusa","year":"2008","journal-title":"Development"},{"key":"2026100620100339400_RSOB250441C11","doi-asserted-by":"crossref","first-page":"344","DOI":"10.1038\/nature18958","article-title":"Asymmetric division of contractile domains couples cell positioning and fate specification","volume":"536","author":"Ma\u00eetre","year":"2016","journal-title":"Nature"},{"key":"2026100620100339400_RSOB250441C12","doi-asserted-by":"crossref","first-page":"435","DOI":"10.1016\/j.devcel.2015.07.004","article-title":"Cortical tension allocates the first inner cells of the mammalian embryo","volume":"34","author":"Samarage","year":"2015","journal-title":"Dev. Cell"},{"key":"2026100620100339400_RSOB250441C13","doi-asserted-by":"crossref","first-page":"303","DOI":"10.1083\/jcb.91.1.303","article-title":"Induction of polarity in mouse 8-cell blastomeres: specificity, geometry, and stability","volume":"91","author":"Johnson","year":"1981","journal-title":"J. Cell Biol."},{"key":"2026100620100339400_RSOB250441C14","doi-asserted-by":"crossref","first-page":"568","DOI":"10.1006\/dbio.1996.0186","article-title":"Ezrin becomes restricted to outer cells following asymmetrical division in the preimplantation mouse embryo","volume":"177","author":"Louvet","year":"1996","journal-title":"Dev. Biol."},{"key":"2026100620100339400_RSOB250441C15","doi-asserted-by":"crossref","first-page":"307","DOI":"10.1016\/j.ydbio.2005.03.001","article-title":"Asymmetric distribution of PAR proteins in the mouse embryo begins at the 8-cell stage during compaction","volume":"282","author":"Vinot","year":"2005","journal-title":"Dev. Biol."},{"key":"2026100620100339400_RSOB250441C16","doi-asserted-by":"crossref","first-page":"eabd2703","DOI":"10.1126\/science.abd2703","article-title":"Developmental clock and mechanism of de novo polarization of the mouse embryo","volume":"370","author":"Zhu","year":"2020","journal-title":"Science"},{"key":"2026100620100339400_RSOB250441C17","doi-asserted-by":"crossref","first-page":"921","DOI":"10.1038\/s41467-017-00977-8","article-title":"Actomyosin polarisation through PLC-PKC triggers symmetry breaking of the mouse embryo","volume":"8","author":"Zhu","year":"2017","journal-title":"Nat. Commun."},{"key":"2026100620100339400_RSOB250441C18","doi-asserted-by":"crossref","first-page":"2813","DOI":"10.1242\/dev.107276","article-title":"Initiation of Hippo signaling is linked to polarity rather than to cell position in the pre-implantation mouse embryo","volume":"141","author":"Anani","year":"2014","journal-title":"Development"},{"key":"2026100620100339400_RSOB250441C19","doi-asserted-by":"crossref","first-page":"505","DOI":"10.1242\/jcs.01666","article-title":"Downregulation of Par3 and aPKC function directs cells towards the ICM in the preimplantation mouse embryo","volume":"118","author":"Plusa","year":"2005","journal-title":"J. Cell Sci."},{"key":"2026100620100339400_RSOB250441C20","doi-asserted-by":"crossref","first-page":"e7117","DOI":"10.1371\/journal.pone.0007117","article-title":"Inactivation of aPKClambda reveals a context dependent allocation of cell lineages in preimplantation mouse embryos","volume":"4","author":"Dard","year":"2009","journal-title":"PLoS ONE"},{"key":"2026100620100339400_RSOB250441C21","doi-asserted-by":"crossref","first-page":"1181","DOI":"10.1016\/j.cub.2013.05.014","article-title":"Polarity-dependent distribution of angiomotin localizes Hippo signaling in preimplantation embryos","volume":"23","author":"Hirate","year":"2013","journal-title":"Curr. Biol."},{"key":"2026100620100339400_RSOB250441C22","doi-asserted-by":"crossref","first-page":"398","DOI":"10.1016\/j.devcel.2009.02.003","article-title":"The Hippo signaling pathway components lats and Yap pattern Tead4 activity to distinguish mouse trophectoderm from inner cell mass","volume":"16","author":"Nishioka","year":"2009","journal-title":"Dev. Cell"},{"key":"2026100620100339400_RSOB250441C23","doi-asserted-by":"crossref","first-page":"e1004618","DOI":"10.1371\/journal.pgen.1004618","article-title":"HIPPO pathway members restrict SOX2 to the inner cell mass where it promotes ICM fates in the mouse blastocyst","volume":"10","author":"Wicklow","year":"2014","journal-title":"PLoS Genet."},{"key":"2026100620100339400_RSOB250441C24","doi-asserted-by":"crossref","first-page":"404","DOI":"10.1038\/s41586-020-2647-4","article-title":"Keratins are asymmetrically inherited fate determinants in the mammalian embryo","volume":"585","author":"Lim","year":"2020","journal-title":"Nature"},{"key":"2026100620100339400_RSOB250441C25","doi-asserted-by":"crossref","first-page":"270","DOI":"10.1016\/j.mod.2007.11.002","article-title":"Tead4 is required for specification of trophectoderm in pre-implantation mouse embryos","volume":"125","author":"Nishioka","year":"2008","journal-title":"Mech. Dev."},{"key":"2026100620100339400_RSOB250441C26","doi-asserted-by":"crossref","first-page":"3827","DOI":"10.1242\/dev.010223","article-title":"Transcription factor TEAD4 specifies the trophectoderm lineage at the beginning of mammalian development","volume":"134","author":"Yagi","year":"2007","journal-title":"Development"},{"key":"2026100620100339400_RSOB250441C27","doi-asserted-by":"crossref","first-page":"15034","DOI":"10.1038\/srep15034","article-title":"The first two cell-fate decisions of preimplantation mouse embryo development are not functionally independent","volume":"5","author":"Mihajlovi\u0107","year":"2015","journal-title":"Sci. Rep."},{"key":"2026100620100339400_RSOB250441C28","doi-asserted-by":"crossref","first-page":"170210","DOI":"10.1098\/rsob.170210","article-title":"The first cell-fate decision of mouse preimplantation embryo development: integrating cell position and polarity","volume":"7","author":"Mihajlovi\u0107","year":"2017","journal-title":"Open Biol."},{"key":"2026100620100339400_RSOB250441C29","doi-asserted-by":"crossref","first-page":"347","DOI":"10.1095\/biolreprod.110.084400","article-title":"Cell polarity regulator PARD6B is essential for trophectoderm formation in the preimplantation mouse embryo","volume":"83","author":"Alarcon","year":"2010","journal-title":"Biol. Reprod."},{"key":"2026100620100339400_RSOB250441C30","doi-asserted-by":"crossref","first-page":"520","DOI":"10.1016\/j.cub.2004.02.061","article-title":"Two PAR6 proteins become asymmetrically localized during establishment of polarity in mouse oocytes","volume":"14","author":"Vinot","year":"2004","journal-title":"Curr. Biol."},{"key":"2026100620100339400_RSOB250441C31","doi-asserted-by":"crossref","first-page":"395","DOI":"10.1242\/dev.038828","article-title":"Gata3 regulates trophoblast development downstream of Tead4 and in parallel to Cdx2","volume":"137","author":"Ralston","year":"2010","journal-title":"Development"},{"key":"2026100620100339400_RSOB250441C32","first-page":"1606","article-title":"Transcription factor AP\u20132\u03b3 induces early Cdx2 expression and represses HIPPO signaling to specify the trophectoderm lineage","volume":"142","author":"Cao","year":"2015","journal-title":"Development"},{"key":"2026100620100339400_RSOB250441C33","doi-asserted-by":"crossref","first-page":"2093","DOI":"10.1242\/dev.01801","article-title":"Cdx2 is required for correct cell fate specification and differentiation of trophectoderm in the mouse blastocyst","volume":"132","author":"Strumpf","year":"2005","journal-title":"Development"},{"key":"2026100620100339400_RSOB250441C34","doi-asserted-by":"crossref","first-page":"593","DOI":"10.1002\/dvg.20335","article-title":"A global double-fluorescent Cre reporter mouse","volume":"45","author":"Muzumdar","year":"2007","journal-title":"Genesis"},{"key":"2026100620100339400_RSOB250441C35","doi-asserted-by":"crossref","first-page":"235","DOI":"10.1016\/j.devcel.2017.01.006","article-title":"The apical domain is required and sufficient for the first lineage segregation in the mouse embryo","volume":"40","author":"Korotkevich","year":"2017","journal-title":"Dev. Cell"},{"key":"2026100620100339400_RSOB250441C36","doi-asserted-by":"crossref","first-page":"544","DOI":"10.1111\/dgd.12235","article-title":"Par-aPKC-dependent and -independent mechanisms cooperatively control cell polarity, Hippo signaling, and cell positioning in 16-cell stage mouse embryos","volume":"57","author":"Hirate","year":"2015","journal-title":"Dev. Growth Differ."},{"key":"2026100620100339400_RSOB250441C37","doi-asserted-by":"crossref","first-page":"142","DOI":"10.1016\/j.ydbio.2014.06.023","article-title":"Inhibition of RHO-ROCK signaling enhances ICM and suppresses TE characteristics through activation of Hippo signaling in the mouse blastocyst","volume":"394","author":"Kono","year":"2014","journal-title":"Dev. Biol."},{"key":"2026100620100339400_RSOB250441C38","doi-asserted-by":"crossref","first-page":"381","DOI":"10.1016\/j.rbmo.2016.06.028","article-title":"Rho-associated protein kinase regulates subcellular localisation of Angiomotin and Hippo-signalling during preimplantation mouse embryo development","volume":"33","author":"Mihajlovi\u0107","year":"2016","journal-title":"Reprod. Biomed. Online"},{"key":"2026100620100339400_RSOB250441C39","article-title":"Asynchronous mouse embryo polarization leads to heterogeneity in cell fate specification","author":"Lamba","year":"2025"},{"key":"2026100620100339400_RSOB250441C40","doi-asserted-by":"crossref","first-page":"4623","DOI":"10.1242\/dev.086645","article-title":"Transcription factor AP\u20132\u03b3 is a core regulator of tight junction biogenesis and cavity formation during mouse early embryogenesis","volume":"139","author":"Choi","year":"2012","journal-title":"Development"},{"key":"2026100620100339400_RSOB250441C41","doi-asserted-by":"crossref","first-page":"4435","DOI":"10.1242\/dev.01316","article-title":"Maternal beta-catenin and E-cadherin in mouse development","volume":"131","author":"De Vries","year":"2004","journal-title":"Development"},{"key":"2026100620100339400_RSOB250441C42","doi-asserted-by":"crossref","first-page":"e230322","DOI":"10.1530\/REP-23-0322","article-title":"TEAD4 regulates KRT8 and YAP in preimplantation embryos in mice but not in cattle","volume":"167","author":"Wu","year":"2024","journal-title":"Reproduction"},{"key":"2026100620100339400_RSOB250441C43","doi-asserted-by":"crossref","first-page":"7362","DOI":"10.1073\/pnas.1201595109","article-title":"Altered subcellular localization of transcription factor TEAD4 regulates first mammalian cell lineage commitment","volume":"109","author":"Home","year":"2012","journal-title":"Proc. Natl Acad. Sci. USA"},{"key":"2026100620100339400_RSOB250441C44","doi-asserted-by":"crossref","first-page":"675","DOI":"10.1016\/j.devcel.2010.02.012","article-title":"Resolution of cell fate decisions revealed by single-cell gene expression analysis from zygote to blastocyst","volume":"18","author":"Guo","year":"2010","journal-title":"Dev. Cell"},{"key":"2026100620100339400_RSOB250441C45","doi-asserted-by":"crossref","first-page":"496","DOI":"10.1016\/j.devcel.2017.05.003","article-title":"Lineage establishment and progression within the inner cell mass of the mouse blastocyst requires FGFR1 and FGFR2","volume":"41","author":"Kang","year":"2017","journal-title":"Dev. Cell"},{"key":"2026100620100339400_RSOB250441C46","doi-asserted-by":"crossref","first-page":"312","DOI":"10.1101\/gad.1833510","article-title":"Sox17 promotes differentiation in mouse embryonic stem cells by directly regulating extraembryonic gene expression and indirectly antagonizing self-renewal","volume":"24","author":"Niakan","year":"2010","journal-title":"Genes Dev."},{"key":"2026100620100339400_RSOB250441C47","doi-asserted-by":"crossref","first-page":"723","DOI":"10.1242\/dev.126.9.723","article-title":"The transcription factor GATA6 is essential for early extraembryonic development","volume":"126","author":"Koutsourakis","year":"1999","journal-title":"Development"},{"key":"2026100620100339400_RSOB250441C48","doi-asserted-by":"crossref","first-page":"3579","DOI":"10.1101\/gad.12.22.3579","article-title":"GATA6 regulates HNF4 and is required for differentiation of visceral endoderm in the mouse embryo","volume":"12","author":"Morrisey","year":"1998","journal-title":"Genes Dev."},{"key":"2026100620100339400_RSOB250441C49","doi-asserted-by":"crossref","first-page":"dev179861","DOI":"10.1242\/dev.179861","article-title":"TEAD4, YAP1 and WWTR1 prevent the premature onset of pluripotency prior to the 16-cell stage","volume":"146","author":"Frum","year":"2019","journal-title":"Development"},{"key":"2026100620100339400_RSOB250441C50","doi-asserted-by":"crossref","first-page":"562","DOI":"10.1038\/nrm2197","article-title":"Intermediate filaments: from cell architecture to nanomechanics","volume":"8","author":"Herrmann","year":"2007","journal-title":"Nat. Rev. Mol. Cell Biol."},{"key":"2026100620100339400_RSOB250441C51","doi-asserted-by":"crossref","first-page":"54","DOI":"10.1038\/nrm1788","article-title":"Function and regulation of Rnd proteins","volume":"7","author":"Chardin","year":"2006","journal-title":"Nat. Rev. Mol. Cell Biol."},{"key":"2026100620100339400_RSOB250441C52","doi-asserted-by":"crossref","first-page":"E1863","DOI":"10.1073\/pnas.1600968113","article-title":"A RhoA and Rnd3 cycle regulates actin reassembly during membrane blebbing","volume":"113","author":"Aoki","year":"2016","journal-title":"Proc. Natl Acad. Sci. USA"},{"key":"2026100620100339400_RSOB250441C53","doi-asserted-by":"crossref","first-page":"1170","DOI":"10.1038\/sj.emboj.7600612","article-title":"RhoE function is regulated by ROCK I-mediated phosphorylation","volume":"24","author":"Riento","year":"2005","journal-title":"EMBO J."},{"key":"2026100620100339400_RSOB250441C54","doi-asserted-by":"crossref","first-page":"7829","DOI":"10.1128\/MCB.24.18.7829-7840.2004","article-title":"RhoE inhibits cell cycle progression and Ras-induced transformation","volume":"24","author":"Villalonga","year":"2004","journal-title":"Mol. Cell Biol."},{"key":"2026100620100339400_RSOB250441C55","doi-asserted-by":"crossref","first-page":"1106","DOI":"10.1016\/S0960-9822(03)00418-4","article-title":"Rnd proteins function as RhoA antagonists by activating p190 RhoGAP","volume":"13","author":"Wennerberg","year":"2003","journal-title":"Curr. Biol."},{"key":"2026100620100339400_RSOB250441C56","doi-asserted-by":"crossref","first-page":"709","DOI":"10.1016\/j.ejcb.2005.04.001","article-title":"Type II keratins precede type I keratins during early embryonic development","volume":"84","author":"Lu","year":"2005","journal-title":"Eur. J. Cell Biol."},{"key":"2026100620100339400_RSOB250441C57","doi-asserted-by":"crossref","first-page":"565","DOI":"10.1242\/dev.101.3.565","article-title":"Cytokeratin filament assembly in the preimplantation mouse embryo","volume":"101","author":"Chisholm","year":"1987","journal-title":"Development"},{"key":"2026100620100339400_RSOB250441C58","doi-asserted-by":"crossref","first-page":"219","DOI":"10.1242\/dev.104.2.219","article-title":"Disruption of the cytokeratin filament network in the preimplantation mouse embryo","volume":"104","author":"Emerson","year":"1988","journal-title":"Development"},{"key":"2026100620100339400_RSOB250441C59","doi-asserted-by":"crossref","first-page":"614","DOI":"10.1016\/j.ydbio.2007.10.054","article-title":"Cdx2 acts downstream of cell polarization to cell-autonomously promote trophectoderm fate in the early mouse embryo","volume":"313","author":"Ralston","year":"2008","journal-title":"Dev. Biol."},{"key":"2026100620100339400_RSOB250441C60","doi-asserted-by":"crossref","first-page":"71","DOI":"10.1007\/978-1-60761-376-3_4","article-title":"Jasplakinolide: an actin-specific reagent that promotes actin polymerization","volume":"586","author":"Holzinger","year":"2009","journal-title":"Methods Mol. Biol."},{"key":"2026100620100339400_RSOB250441C61","doi-asserted-by":"crossref","first-page":"e22906","DOI":"10.7554\/eLife.22906","article-title":"Position- and Hippo signaling-dependent plasticity during lineage segregation in the early mouse embryo","volume":"6","author":"Posfai","year":"2017","journal-title":"eLife"},{"key":"2026100620100339400_RSOB250441C62","doi-asserted-by":"crossref","first-page":"2251","DOI":"10.1038\/ncomms3251","article-title":"Angiomotin prevents pluripotent lineage differentiation in mouse embryos via Hippo pathway-dependent and -independent mechanisms","volume":"4","author":"Leung","year":"2013","journal-title":"Nat. Commun."},{"key":"2026100620100339400_RSOB250441C63","doi-asserted-by":"crossref","first-page":"339","DOI":"10.1038\/35070009","article-title":"Membrane blebbing during apoptosis results from caspase\u2013mediated activation of ROCK I","volume":"3","author":"Coleman","year":"2001","journal-title":"Nat. Cell Biol."},{"key":"2026100620100339400_RSOB250441C64","doi-asserted-by":"crossref","first-page":"3571","DOI":"10.1007\/s00018-019-03153-x","article-title":"Death for life: a path from apoptotic signaling to tissue-scale effects of apoptotic epithelial extrusion","volume":"76","author":"Gagliardi","year":"2019","journal-title":"Cell Mol. Life Sci."},{"key":"2026100620100339400_RSOB250441C65","doi-asserted-by":"crossref","first-page":"139","DOI":"10.1016\/j.devcel.2019.05.024","article-title":"Epiblast formation by TEAD-YAP-dependent expression of pluripotency factors and competitive elimination of unspecified cells","volume":"50","author":"Hashimoto","year":"2019","journal-title":"Dev. Cell"},{"key":"2026100620100339400_RSOB250441C66","doi-asserted-by":"crossref","first-page":"632","DOI":"10.1038\/nbt.4166","article-title":"Efficient generation of targeted large insertions by microinjection into two-cell-stage mouse embryos","volume":"36","author":"Gu","year":"2018","journal-title":"Nat. Biotechnol."},{"key":"2026100620100339400_RSOB250441C67","doi-asserted-by":"crossref","first-page":"W332","DOI":"10.1093\/nar\/gkq317","article-title":"E-RNAi: a web application for the multi-species design of RNAi reagents\u20132010 update","volume":"38","author":"Horn","year":"2010","journal-title":"Nucleic. Acids Res."},{"key":"2026100620100339400_RSOB250441C68","doi-asserted-by":"crossref","first-page":"2692","DOI":"10.1101\/gad.486108","article-title":"Role of Cdx2 and cell polarity in cell allocation and specification of trophectoderm and inner cell mass in the mouse embryo","volume":"22","author":"Jedrusik","year":"2008","journal-title":"Genes Dev."},{"key":"2026100620100339400_RSOB250441C69","doi-asserted-by":"crossref","first-page":"85","DOI":"10.1016\/0092-8674(95)90373-9","article-title":"Expression cloning of Siamois, a Xenopus homeobox gene expressed in dorsal-vegetal cells of blastulae and able to induce a complete secondary axis","volume":"81","author":"Lemaire","year":"1995","journal-title":"Cell"},{"key":"2026100620100339400_RSOB250441C70","doi-asserted-by":"crossref","first-page":"369","DOI":"10.1016\/j.cub.2009.01.064","article-title":"Regulation of APC\/C activity in oocytes by a Bub1-dependent spindle assembly checkpoint","volume":"19","author":"McGuinness","year":"2009","journal-title":"Curr. Biol."},{"key":"2026100620100339400_RSOB250441C71","doi-asserted-by":"crossref","first-page":"788","DOI":"10.1038\/s42003-021-02290-z","article-title":"p38-MAPK-mediated translation regulation during early blastocyst development is required for primitive endoderm differentiation in mice","volume":"4","author":"Bora","year":"2021","journal-title":"Commun. Biol."},{"key":"2026100620100339400_RSOB250441C72","doi-asserted-by":"crossref","first-page":"676","DOI":"10.1038\/nmeth.2019","article-title":"Fiji: an open-source platform for biological-image analysis","volume":"9","author":"Schindelin","year":"2012","journal-title":"Nat. Methods"},{"key":"2026100620100339400_RSOB250441C73","unstructured":"R Core Team\n          . 2024R: a language and environment for statistical computing. https:\/\/www.r-project.org."},{"key":"2026100620100339400_RSOB250441C74","unstructured":"Posit\n          . 2025RStudio: integrated development environment for R. Version 2024.04.2+764. https:\/\/posit.co\/products\/open-source\/rstudio."},{"key":"2026100620100339400_RSOB250441C75","doi-asserted-by":"crossref","first-page":"1","DOI":"10.18637\/jss.v025.i01","article-title":"FactoMineR: an R package for multivariate analysis","volume":"25","author":"L\u00ea","year":"2008","journal-title":"J. Stat. Softw."},{"key":"2026100620100339400_RSOB250441C76","doi-asserted-by":"crossref","unstructured":"Kassambara\n              A\n            \n          . 2016factoextra: extract and visualize the results of multivariate data analyses. Version 1.0.7. https:\/\/kassambara.r-universe.dev\/factoextra.","DOI":"10.32614\/CRAN.package.factoextra"},{"key":"2026100620100339400_RSOB250441C77","doi-asserted-by":"crossref","first-page":"jcs252924","DOI":"10.1242\/jcs.252924","article-title":"CDC25B is required for the metaphase I-metaphase II transition in mouse oocytes","volume":"135","author":"Ferencova","year":"2022","journal-title":"J. Cell Sci."},{"key":"2026100620100339400_RSOB250441C78","doi-asserted-by":"crossref","first-page":"402","DOI":"10.1006\/meth.2001.1262","article-title":"Analysis of relative gene expression data using real-time quantitative PCR and the 2\u2212\u0394\u0394CT method","volume":"25","author":"Livak","year":"2001","journal-title":"Methods"},{"key":"2026100620100339400_RSOB250441C79","article-title":"Supplementary material from: TEAD4 Regulates apical domain homeostasis and cell-positioning to maintain the trophectoderm lineage during mouse blastocyst development","author":"Collier","year":"2026"}],"container-title":["Open Biology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/royalsocietypublishing.org\/rsob\/article-pdf\/doi\/10.1098\/rsob.250441\/6169301\/rsob.250441.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/royalsocietypublishing.org\/rsob\/article-pdf\/doi\/10.1098\/rsob.250441\/6169301\/rsob.250441.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T00:10:51Z","timestamp":1791331851000},"score":1,"resource":{"primary":{"URL":"https:\/\/royalsocietypublishing.org\/rsob\/article\/16\/10\/250441\/483543\/TEAD4-Regulates-apical-domain-homeostasis-and-cell"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,10,7]]},"references-count":79,"journal-issue":{"issue":"10","published-print":{"date-parts":[[2026,10,7]]}},"URL":"https:\/\/doi.org\/10.1098\/rsob.250441","relation":{},"ISSN":["2046-2441"],"issn-type":[{"value":"2046-2441","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,10,7]]},"article-number":"250441"}}