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CDK-opposing phosphatases PP1, PP2A-B55 and PP2A-B56 are regulators of mitosis. A screen for differentially phosphorylated sites in a \u0394PP1dis2 genetic background in Schizosaccharomyces pombe identified phosphorylation of T73 or T75 in the regulatory B56Par1 subunit. The B56Par1.T73T75 phosphorylation is directly mediated by CDK1-Cyclin B, and a phospho-mimetic mutation increased while a non-phosphorylatable mutation reduced PP2A-B56Par1 phosphatase activity. Blocking B56Par1.T73T75 phosphorylation reduced cell length in unperturbed divisions from 14 to 12 \u00b5m, without causing pleiotropic defects. Therefore, blocking phosphorylation at T73T75 alone prematurely unlocked amplification of the CDK1\/Cdc25\/Wee1 feedback loop, advancing cells into mitosis. Signalling from T73T75 reveals for the first time that timely mitotic commitment in unperturbed cycles is mediated by PP2A-B56.<\/jats:p>","DOI":"10.1098\/rsob.260089","type":"journal-article","created":{"date-parts":[[2026,8,19]],"date-time":"2026-08-19T00:13:12Z","timestamp":1787098392000},"source":"Crossref","is-referenced-by-count":1,"title":["CDK1-Cyclin B mediated phosphorylation of PP2A-B56 on B56Par1.T73T75 controls mitotic entry timing and\n                    <i>Schizosaccharomyces pombe<\/i>\n                    cell size"],"prefix":"10.1098","volume":"16","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7253-6512","authenticated-orcid":false,"given":"Lenka","family":"Halova","sequence":"first","affiliation":[{"name":"Cell Division, Cancer Research UK Manchester Institute, The University of Manchester 1 , Manchester, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yvonne","family":"Connolly","sequence":"additional","affiliation":[{"name":"Biological Mass Spectrometry, Cancer Research UK Manchester Institute, The University of Manchester 2 , Manchester, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Duncan","family":"Smith","sequence":"additional","affiliation":[{"name":"Biological Mass Spectrometry, Cancer Research UK Manchester Institute, The University of Manchester 2 , Manchester, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0729-9335","authenticated-orcid":false,"given":"Janni","family":"Petersen","sequence":"additional","affiliation":[{"name":"Flinders Centre for Innovation in Cancer, Flinders University College of Medicine and Public Health 3 , Adelaide, South Australia, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5858-9415","authenticated-orcid":false,"given":"Iain","family":"Hagan","sequence":"additional","affiliation":[{"name":"Cell Division, Cancer Research UK Manchester Institute, The University of Manchester 1 , Manchester, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"175","published-online":{"date-parts":[[2026,8,19]]},"reference":[{"key":"2026081820130580800_RSOB260089C1","doi-asserted-by":"crossref","first-page":"503","DOI":"10.1038\/344503a0","article-title":"Universal control mechanism regulating onset of M-phase","volume":"344","author":"Nurse","year":"1990","journal-title":"Nature"},{"key":"2026081820130580800_RSOB260089C2","doi-asserted-by":"crossref","first-page":"345","DOI":"10.1016\/S0168-9525(96)80016-3","article-title":"A quantitative model for the cdc2 control of S phase and mitosis in fission yeast","volume":"12","author":"Stern","year":"1996","journal-title":"Trends Genet."},{"key":"2026081820130580800_RSOB260089C3","doi-asserted-by":"crossref","first-page":"680","DOI":"10.1038\/347680a0","article-title":"Distinct nuclear and spindle pole body population of cyclin-cdc2 in fission yeast","volume":"347","author":"Alfa","year":"1990","journal-title":"Nature"},{"key":"2026081820130580800_RSOB260089C4","doi-asserted-by":"crossref","first-page":"2627","DOI":"10.1242\/jcs.114.14.2627","article-title":"In vivo localisation of fission yeast cyclin-dependent kinase cdc2p and cyclin B cdc13p during mitosis and meiosis","volume":"114","author":"Decottignies","year":"2001","journal-title":"J. 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