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Soc. open sci."],"published-print":{"date-parts":[[2020,7]]},"abstract":"<jats:p>In the field of public health, treatment of multidrug-resistant (MDR) bacterial infection is a great challenge. Herein, we provide a solution to this problem with the use of graphene oxide-silver (GO-Ag) nanocomposites as antibacterial agent. Following established protocols, silver nanoparticles were grown on graphene oxide sheets. Then, a series of<jats:italic>in vitro<\/jats:italic>studies were conducted to validate the antibacterial efficiency of the GO-Ag nanocomposites against clinical MDR<jats:italic>Escherichia coli<\/jats:italic>(<jats:italic>E. coli<\/jats:italic>) strains. GO-Ag nanocomposites showed the highest antibacterial efficiency among tested antimicrobials (graphene oxide, silver nanoparticles, GO-Ag), and synergetic antibacterial effect was observed in GO-Ag nanocomposites treated group. Treatment with 14.0 \u00b5g ml<jats:sup>\u22121<\/jats:sup>GO-Ag could greatly inhibit bacteria growth; remaining bacteria viabilities were 4.4% and 4.1% for MDR-1 and MDR-2<jats:italic>E. coli<\/jats:italic>bacteria, respectively. In addition, with assistance of photothermal effect, effective sterilization could be achieved using GO-Ag nanocomposites as low as 7.0 \u00b5g ml<jats:sup>\u22121<\/jats:sup>. Fluorescence imaging and morphology characterization uncovered that bacteria integrity was disrupted after GO-Ag nanocomposites treatment. Cytotoxicity results of GO-Ag using human-derived cell lines (HEK 293T, Hep G2) suggested more than 80% viability remained at 7.0 \u00b5g ml<jats:sup>\u22121<\/jats:sup>. All the results proved that GO-Ag nanocomposites are efficient antibacterial agent against multidrug-resistant<jats:italic>E. coli<\/jats:italic>.<\/jats:p>","DOI":"10.1098\/rsos.192019","type":"journal-article","created":{"date-parts":[[2020,7,22]],"date-time":"2020-07-22T07:06:16Z","timestamp":1595401576000},"page":"192019","update-policy":"https:\/\/doi.org\/10.1098\/crossmark-policy","source":"Crossref","is-referenced-by-count":23,"title":["Photothermal-assisted antibacterial application of graphene oxide-Ag nanocomposites against clinically isolated multi-drug resistant<i>Escherichia coli<\/i>"],"prefix":"10.1098","volume":"7","author":[{"given":"Yuqing","family":"Chen","sequence":"first","affiliation":[{"name":"Children's ENT Department, The Affiliated Wuxi Maternity and Child Health Care Hospital of Nanjing Medical University, 214122 Wuxi, People's Republic of China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wei","family":"Wu","sequence":"additional","affiliation":[{"name":"Cardiothoracic Surgery Department, The Affiliated Wuxi No. 2 People's Hospital of Nanjing Medical University, 214002 Wuxi, People's Republic of China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zeqiao","family":"Xu","sequence":"additional","affiliation":[{"name":"Urology Surgery Department, The Affiliated Wuxi People's Hospital of Nanjing Medical University, 214122 Wuxi, People's Republic of China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Cheng","family":"Jiang","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine, The Affiliated Wuxi People's Hospital of Nanjing Medical University, 214122 Wuxi, People's Republic of China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shuang","family":"Han","sequence":"additional","affiliation":[{"name":"School of Biotechnology, Jiangnan University, 214122 Wuxi, People's Republic of China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jun","family":"Ruan","sequence":"additional","affiliation":[{"name":"Urology Surgery Department, The Affiliated Wuxi People's Hospital of Nanjing Medical University, 214122 Wuxi, People's Republic of China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4244-852X","authenticated-orcid":true,"given":"Yong","family":"Wang","sequence":"additional","affiliation":[{"name":"Urology Surgery Department, The Affiliated Wuxi People's Hospital of Nanjing Medical University, 214122 Wuxi, People's Republic of China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"175","published-online":{"date-parts":[[2020,7,22]]},"reference":[{"key":"e_1_3_6_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1473-3099(15)00466-1"},{"key":"e_1_3_6_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajic.2006.05.219"},{"key":"e_1_3_6_4_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-0691.2011.03570.x"},{"key":"e_1_3_6_5_2","doi-asserted-by":"publisher","DOI":"10.1097\/01.CCM.0000245666.28867.C6"},{"key":"e_1_3_6_6_2","doi-asserted-by":"publisher","DOI":"10.1097\/QCO.0b013e328354f192"},{"key":"e_1_3_6_7_2","doi-asserted-by":"publisher","DOI":"10.2217\/fmb-2015-0014"},{"key":"e_1_3_6_8_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrmicro3432"},{"key":"e_1_3_6_9_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa011265"},{"key":"e_1_3_6_10_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2672.1977.tb00773.x"},{"key":"e_1_3_6_11_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.48.10.3996-4001.2004"},{"key":"e_1_3_6_12_2","doi-asserted-by":"publisher","DOI":"10.4315\/0362-028X-61.11.1511"},{"key":"e_1_3_6_13_2","doi-asserted-by":"publisher","DOI":"10.1080\/00480169.1991.35678"},{"key":"e_1_3_6_14_2","doi-asserted-by":"publisher","DOI":"10.1086\/428451"},{"key":"e_1_3_6_15_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.00763-07"},{"key":"e_1_3_6_16_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1220761"},{"key":"e_1_3_6_17_2","first-page":"2767","article-title":"Antimicrobial applications of nanotechnology: methods and literature","volume":"7","author":"Seil JT","year":"2012","journal-title":"Int. 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