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Soc. open sci."],"published-print":{"date-parts":[[2022,11]]},"abstract":"<jats:p>\n                    The discovery of novel antihelmintic molecules to combat the development and spread of schistosomiasis, a disease caused by several\n                    <jats:italic>Schistosoma<\/jats:italic>\n                    flatworm species, mobilizes significant research efforts worldwide. With a limited number of biochemical assays for measuring the viability of adult worms, the antischistosomicidal activity of molecules is usually evaluated by a microscopic observation of worm mobility and\/or integrity upon drug exposure. Even if these phenotypical assays enable multiple parameters analysis, they are often conducted during several days and need to be associated with image-based analysis to minimized subjectivity. We describe here a self-purifying microfluidic system enabling the selection of healthy adult worms and the identification of molecules acting instantly on the parasite. The worms are assayed in a dynamic environment that eliminates unhealthy worms that cannot attach firmly to the chip walls prior to being exposed to the drug. The detachment of the worms is also used as second step readout for identifying active compounds. We have validated this new fluidic screening approach using the two major antihelmintic drugs, praziquantel and artemisinin. The reported dynamic system is simple to produce and to parallelize. Importantly, it enables a quick and sensitive detection of antischistosomal compounds in no more than one hour.\n                  <\/jats:p>","DOI":"10.1098\/rsos.220648","type":"journal-article","created":{"date-parts":[[2022,11,30]],"date-time":"2022-11-30T03:06:40Z","timestamp":1669777600000},"update-policy":"https:\/\/doi.org\/10.1098\/crossmark-policy","source":"Crossref","is-referenced-by-count":5,"title":["A self-purifying microfluidic system for identifying drugs acting against adult schistosomes"],"prefix":"10.1098","volume":"9","author":[{"given":"Vincent","family":"Girod","sequence":"first","affiliation":[{"name":"University of Lille, CNRS, Inserm, CHU Lille, UMR9020-U1277 \u2013 CANTHER \u2013 Cancer Heterogeneity Plasticity and Resistance to Therapies, Lille F-59000, France"},{"name":"CNRS, University of Tokyo, IRL2820 \u2013 LIMMS, Lille F-59000, France"},{"name":"University of Lille, CNRS, Inserm, CHU Lille, Institut Pasteur de Lille, U1019-UMR9017 \u2013 Center for Infection and Immunity of Lille, F-59000 Lille, France"},{"name":"University of Lille, CNRS, UPHF, JUNIA, CLI, UMR 8520 \u2013 IEMN \u2013 Institut d'Electronique, de Micro\u00e9lectronique et de Nanotechnologie, Villeneuve d'Ascq F-59650, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Robin","family":"Houssier","sequence":"additional","affiliation":[{"name":"University of Lille, CNRS, Inserm, CHU Lille, UMR9020-U1277 \u2013 CANTHER \u2013 Cancer Heterogeneity Plasticity and Resistance to Therapies, Lille F-59000, France"},{"name":"University of Lille, CNRS, Inserm, CHU Lille, Institut Pasteur de Lille, U1019-UMR9017 \u2013 Center for Infection and Immunity of Lille, F-59000 Lille, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Karin","family":"Sahmer","sequence":"additional","affiliation":[{"name":"University of Lille, IMT Lille Douai, University of Artois, JUNIA, ULR 4515 \u2013 LGCgE, Laboratoire de G\u00e9nie Civil et g\u00e9o-Environnement, F-59000 Lille, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marie-Jos\u00e9","family":"Ghoris","sequence":"additional","affiliation":[{"name":"University of Lille, CNRS, Inserm, CHU Lille, Institut Pasteur de Lille, U1019-UMR9017 \u2013 Center for Infection and Immunity of Lille, F-59000 Lille, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"St\u00e9phanie","family":"Caby","sequence":"additional","affiliation":[{"name":"University of Lille, CNRS, Inserm, CHU Lille, Institut Pasteur de Lille, U1019-UMR9017 \u2013 Center for Infection and Immunity of Lille, F-59000 Lille, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Oleg","family":"Melnyk","sequence":"additional","affiliation":[{"name":"University of Lille, CNRS, Inserm, CHU Lille, Institut Pasteur de Lille, U1019-UMR9017 \u2013 Center for Infection and Immunity of Lille, F-59000 Lille, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Colette","family":"Dissous","sequence":"additional","affiliation":[{"name":"University of Lille, CNRS, Inserm, CHU Lille, Institut Pasteur de Lille, U1019-UMR9017 \u2013 Center for Infection and Immunity of Lille, F-59000 Lille, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vincent","family":"Senez","sequence":"additional","affiliation":[{"name":"University of Lille, CNRS, Inserm, CHU Lille, UMR9020-U1277 \u2013 CANTHER \u2013 Cancer Heterogeneity Plasticity and Resistance to Therapies, Lille F-59000, France"},{"name":"CNRS, University of Tokyo, IRL2820 \u2013 LIMMS, Lille F-59000, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8360-7497","authenticated-orcid":true,"given":"J\u00e9r\u00f4me","family":"Vicogne","sequence":"additional","affiliation":[{"name":"University of Lille, CNRS, Inserm, CHU Lille, Institut Pasteur de Lille, U1019-UMR9017 \u2013 Center for Infection and Immunity of Lille, F-59000 Lille, France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"175","published-online":{"date-parts":[[2022,11,30]]},"reference":[{"key":"e_1_3_7_2_2","unstructured":"WHO. 2022 Schistosomiasis . 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