{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,20]],"date-time":"2026-08-20T16:38:52Z","timestamp":1787243932209,"version":"build-2736575974"},"reference-count":69,"publisher":"The Royal Society","issue":"10","license":[{"start":{"date-parts":[[2023,10,1]],"date-time":"2023-10-01T00:00:00Z","timestamp":1696118400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/royalsociety.org\/journals\/ethics-policies\/data-sharing-mining\/"}],"funder":[{"name":"European Research Council","award":["336306"],"award-info":[{"award-number":["336306"]}]}],"content-domain":{"domain":["royalsocietypublishing.org"],"crossmark-restriction":true},"short-container-title":["R. Soc. open sci."],"published-print":{"date-parts":[[2023,10]]},"abstract":"<jats:p>\n                    Mechanical loading is critical for collagen network maintenance and remodelling in adult skeletal tissues, but the role of loading in collagen network formation during development is poorly understood. We test the hypothesis that mechanical loading is necessary for the onset and maturation of spatial localization and structure of collagens in prenatal cartilage and bone, using\n                    <jats:italic>in vivo<\/jats:italic>\n                    and\n                    <jats:italic>in vitro<\/jats:italic>\n                    mouse models of altered loading. The majority of collagens studied was aberrant in structure or localization, or both, when skeletal muscle was absent\n                    <jats:italic>in vivo<\/jats:italic>\n                    . Using\n                    <jats:italic>in vitro<\/jats:italic>\n                    bioreactor culture system, we demonstrate that mechanical loading directly modulates the spatial localization and structure of collagens II and X. Furthermore, we show that mechanical loading\n                    <jats:italic>in vitro<\/jats:italic>\n                    rescues aspects of the development of collagens II and X from the effects of fetal immobility. In conclusion, our findings show that mechanical loading is a critical determinant of collagen network establishment during prenatal skeletal development.\n                  <\/jats:p>","DOI":"10.1098\/rsos.231023","type":"journal-article","created":{"date-parts":[[2023,10,18]],"date-time":"2023-10-18T03:05:56Z","timestamp":1697598356000},"update-policy":"https:\/\/doi.org\/10.1098\/crossmark-policy","source":"Crossref","is-referenced-by-count":7,"title":["Mechanical loading due to muscle movement regulates establishment of the collagen network in the developing murine skeleton"],"prefix":"10.1098","volume":"10","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0455-5452","authenticated-orcid":true,"given":"Saima","family":"Ahmed","sequence":"first","affiliation":[{"name":"Department of Bioengineering, Imperial College London, London, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrew 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