{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,4]],"date-time":"2026-01-04T04:52:45Z","timestamp":1767502365255,"version":"3.48.0"},"reference-count":23,"publisher":"The Royal Society","issue":"1254","license":[{"start":{"date-parts":[[1986,10,22]],"date-time":"1986-10-22T00:00:00Z","timestamp":530323200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/royalsociety.org\/journals\/ethics-policies\/data-sharing-mining\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[1986,10,22]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>In attempts to transform and immortalize human cell cultures, skin fibroblasts from normal donors of different ages, from patients with the premature ageing diseases Werner\u2019s syndrome (WS) and progeria (PR), and from donors with the cancer-prone diseases ataxia telangiectasia (AT), Bloom\u2019s syndrome (BS) and Fanconi\u2019s anaemia (FA), were infected with SV40 virus and their growth monitored thereafter. Lesch\u2013Nyhan (LN) fibroblasts were also infected. SV40-infected cultures from two normal and from WS, AT and LN donors attained a spectrum of transformed properties, high mitotic activity at confluence, presence of T-antigen, anchorage independence and altered morphology. Most of these pretransformed cultures died in the crisis period. However, two cultures from the WS and LN patients survived the crisis period and have now been grown to more than 200 passages. For the LN culture the crisis period was at least 200 days. Both permanent lines retain the properties of pretransformed cells, but differ in their modal chromosome number and ability to grow in methionine-free medium. It can be concluded from these experiments that transformation by SV40 to permanent lines is a rare event in human skin fibroblasts, even when these cells were taken from patients predisposed to form cancers.<\/jats:p>","DOI":"10.1098\/rspb.1986.0070","type":"journal-article","created":{"date-parts":[[2006,12,15]],"date-time":"2006-12-15T17:20:43Z","timestamp":1166203243000},"page":"1-12","source":"Crossref","is-referenced-by-count":18,"title":["The susceptibility of Werner\u2019s syndrome and other human skin fibroblasts to SV40-induced transformation and immortalization"],"prefix":"10.1098","volume":"229","author":[{"given":"L. I.","family":"Huschtscha","sequence":"first","affiliation":[{"name":"Genetics Division, National Institute for Medical Research, The Ridgeway, Mill Hill, London NW7 1AA, U. K."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"K. V. A.","family":"Thompson","sequence":"additional","affiliation":[{"name":"Genetics Division, National Institute for Medical Research, The Ridgeway, Mill Hill, London NW7 1AA, U. K."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Robin","family":"Holliday","sequence":"additional","affiliation":[{"name":"Genetics Division, National Institute for Medical Research, The Ridgeway, Mill Hill, London NW7 1AA, U. K."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"175","reference":[{"key":"p_1","doi-asserted-by":"crossref","unstructured":"Chen T. R. 1977 In situ detection of mycoplasma contam ination in cell cultures by fluorescent Hoescht 33258 strain.","DOI":"10.1016\/0014-4827(77)90089-1"},{"key":"p_2","unstructured":"Croce C. Knowles B. B. & Koprowski H. 1983"},{"key":"p_3","unstructured":"ExplCell Res. 104 255-262. Preferential retention of the hum an"},{"key":"p_4","unstructured":"chromosome C-7 (thymidine kinase deficient) mouse hybrid cells. 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J. biol","volume":"193","author":"Lowry 0 .","year":"1951","journal-title":"Chem."},{"key":"p_12","first-page":"105","article-title":"Immortalisation of human Lesch-Nyhan fibroblasts following infection with SV40","volume":"53","author":"Liibbe L.","year":"1983","journal-title":"Arch. Geschumlstforsch"},{"key":"p_13","doi-asserted-by":"crossref","unstructured":"Matsumura T. Nagata M. Konishi R. & Goto M. 1985 Studies of SV40-infected Werner syndrome fibroblasts. In Werner's syndrome and human ageing (ed. D. Salk G. M. Martin & Y. Fujiwara) pp. 313-330. New York and London: Plenum Press.","DOI":"10.1007\/978-1-4684-7853-2_15"},{"key":"p_14","first-page":"530","article-title":"Efficient immortalisation and morphological transform ation of human fibroblasts by transfection with SV40 DNA linked to a dom inant marker","volume":"162","author":"Mayne L. 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Ini","volume":"37","author":"Namba M.","year":"1986","journal-title":"J. Cancer"},{"key":"p_19","doi-asserted-by":"publisher","DOI":"10.1007\/BF02618025"},{"key":"p_20","first-page":"317","article-title":"Hum an brain in tissue culture. III. PML-SV40 induced transform ation of brain cells and establishment of permanent lines. J. comp","volume":"161","author":"Santoli D.","year":"1975","journal-title":"Neurol."},{"key":"p_21","doi-asserted-by":"publisher","DOI":"10.1126\/science.153.3741.1252"},{"key":"p_22","doi-asserted-by":"publisher","DOI":"10.3181\/00379727-124-31974"},{"key":"p_23","first-page":"271","article-title":"Variable susceptibility of human cell strains to SV40 transformation","volume":"29","author":"Todaro G. J.","year":"1968","journal-title":"Nat. Cancer Inst. Monogr."}],"container-title":["Proceedings of the Royal Society of London. Series B. 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