{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,4]],"date-time":"2026-10-04T13:55:59Z","timestamp":1791122159506,"version":"4.1.0"},"reference-count":22,"publisher":"The Royal Society","issue":"1590","license":[{"start":{"date-parts":[[2006,5,7]],"date-time":"2006-05-07T00:00:00Z","timestamp":1146960000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/royalsociety.org\/journals\/ethics-policies\/data-sharing-mining\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Proc. R. Soc. B."],"published-print":{"date-parts":[[2006,5,7]]},"abstract":"<jats:p>\n            The cytoplasmic dye carboxyfluorescein diacetate succinimidyl ester (CFSE) is used to quantify cell kinetics. It is particularly important in studies of lymphocyte homeostasis where its labelling of cells irrespective of their stage in the cell cycle makes it preferable to deuterated glucose and BrdU which only label dividing cells and thus produce unrepresentative results. In the past, experiments have been limited by the need to obtain a clear separation of CFSE peaks forcing scientists to adopt a strategy of\n            <jats:italic>in vitro<\/jats:italic>\n            labelling of cells followed by their injection into the host. Here we develop a framework for analysis of\n            <jats:italic>in vivo<\/jats:italic>\n            CFSE labelling data. This enables us to estimate the rate of proliferation and death of lymphocytes\n            <jats:italic>in situ<\/jats:italic>\n            , and thus represents a considerable advance over current procedures. We illustrate this approach using\n            <jats:italic>in vivo<\/jats:italic>\n            CFSE labelling of B lymphocytes in sheep.\n          <\/jats:p>","DOI":"10.1098\/rspb.2005.3432","type":"journal-article","created":{"date-parts":[[2006,2,1]],"date-time":"2006-02-01T20:20:33Z","timestamp":1138825233000},"page":"1165-1171","source":"Crossref","is-referenced-by-count":36,"title":["Quantifying lymphocyte kinetics\n            <i>in vivo<\/i>\n            using carboxyfluorescein diacetate succinimidyl ester"],"prefix":"10.1098","volume":"273","author":[{"given":"Becca","family":"Asquith","sequence":"first","affiliation":[{"name":"Department of Immunology, Imperial College LondonNorfolk Place, London W2 1PG, UK"},{"name":"Department of Zoology, University of OxfordSouth Parks Road, Oxford OX1 3PS, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christophe","family":"Debacq","sequence":"additional","affiliation":[{"name":"Molecular and Cellular Biology, FUSAGB5030 Gembloux, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Arnaud","family":"Florins","sequence":"additional","affiliation":[{"name":"Molecular and Cellular Biology, FUSAGB5030 Gembloux, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nicolas","family":"Gillet","sequence":"additional","affiliation":[{"name":"Molecular and Cellular Biology, FUSAGB5030 Gembloux, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Teresa","family":"Sanchez-Alcaraz","sequence":"additional","affiliation":[{"name":"Molecular and Cellular Biology, FUSAGB5030 Gembloux, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Angelina","family":"Mosley","sequence":"additional","affiliation":[{"name":"Department of Immunology, Imperial College LondonNorfolk Place, London W2 1PG, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luc","family":"Willems","sequence":"additional","affiliation":[{"name":"Molecular and Cellular Biology, FUSAGB5030 Gembloux, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"175","published-online":{"date-parts":[[2006,5,7]]},"reference":[{"key":"e_1_3_2_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1471-4906(02)02337-2"},{"key":"e_1_3_2_3_1","doi-asserted-by":"publisher","DOI":"10.1098\/rspb.2002.2316"},{"key":"e_1_3_2_4_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.170.5.2479"},{"key":"e_1_3_2_5_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.142100999"},{"key":"e_1_3_2_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jim.2005.01.011"},{"key":"e_1_3_2_7_1","doi-asserted-by":"publisher","DOI":"10.1038\/79782"},{"key":"e_1_3_2_8_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.7123245"},{"key":"e_1_3_2_9_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1521-4141(199805)28:05<1597::AID-IMMU1597>3.0.CO;2-Y"},{"key":"e_1_3_2_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0167-5699(99)01529-7"},{"key":"e_1_3_2_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jtbi.2004.04.011"},{"key":"e_1_3_2_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/0022-1759(94)90236-4"},{"key":"e_1_3_2_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0091-679X(01)63021-8"},{"key":"e_1_3_2_14_1","doi-asserted-by":"publisher","DOI":"10.1002\/eji.200324295"},{"key":"e_1_3_2_15_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.279.5354.1223"},{"key":"e_1_3_2_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-5193(03)00245-5"},{"key":"e_1_3_2_17_1","doi-asserted-by":"publisher","DOI":"10.1002\/eji.1830210207"},{"key":"e_1_3_2_18_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.242358099"},{"key":"e_1_3_2_19_1","doi-asserted-by":"publisher","DOI":"10.1093\/intimm\/dxg016"},{"key":"e_1_3_2_20_1","doi-asserted-by":"publisher","DOI":"10.1002\/eji.1830200812"},{"key":"e_1_3_2_21_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.70.4.1263"},{"key":"e_1_3_2_22_1","doi-asserted-by":"publisher","DOI":"10.1038\/76907"},{"key":"e_1_3_2_23_1","doi-asserted-by":"publisher","DOI":"10.1093\/intimm\/7.10.1607"}],"container-title":["Proceedings 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