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Having recently characterized the cue to gregarious settlement of<jats:italic>Balanus amphitrite<\/jats:italic>, the settlement-inducing protein complex (SIPC), we use two polyclonal antibodies to examine the tissue distribution and ontogenetic expression of this glycoprotein. These antibodies were raised against two separate peptides located near the N- and C-termini of the SIPC and were used to detect the glycoprotein by western blotting and immunohistochemistry. By<jats:italic>in situ<\/jats:italic>hybridization we also show that the SIPC mRNA co-occurs with the expressed glycoprotein in the cuticles of<jats:italic>both<\/jats:italic>nauplius and cypris larval stages and the adult. In the larvae, the SIPC is expressed most strongly in the mouthparts and the hindgut of the stage 2 nauplius and in the thoracopods, antennules and bivalved carapace of the cyprid. In adult<jats:italic>B. amphitrite<\/jats:italic>, the expressed SIPC is present in protein extracts of the shell and in all organs that are lined by cuticular tissues. We suggest that the SIPC is produced by the epidermal cells that secrete the cuticle and discuss these observations with regard to earlier studies and the role of the SIPC as a contact pheromone.<\/jats:p>","DOI":"10.1098\/rspb.2006.3649","type":"journal-article","created":{"date-parts":[[2006,8,14]],"date-time":"2006-08-14T19:14:34Z","timestamp":1155582874000},"page":"2721-2728","source":"Crossref","is-referenced-by-count":57,"title":["Locating the barnacle settlement pheromone: spatial and ontogenetic expression of the settlement-inducing protein complex of<i>Balanus amphitrite<\/i>"],"prefix":"10.1098","volume":"273","author":[{"given":"Catherine","family":"Dreanno","sequence":"first","affiliation":[{"name":"School of Marine Science and Technology, University of NewcastleNewcastle upon Tyne NE1 7RU, UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Richard R","family":"Kirby","sequence":"additional","affiliation":[{"name":"School of Biological Sciences, University of PlymouthPlymouth PL4 8AA, UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anthony S","family":"Clare","sequence":"additional","affiliation":[{"name":"School of Marine Science and Technology, University of NewcastleNewcastle upon Tyne NE1 7RU, UK"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"175","published-online":{"date-parts":[[2006,8,2]]},"reference":[{"key":"e_1_3_2_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0749.2005.00217.x"},{"key":"e_1_3_2_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0145-305X(99)00018-X"},{"key":"e_1_3_2_4_1","first-page":"281","article-title":"Shell structure in sessile barnacles","volume":"104","author":"Bourget E","year":"1977","journal-title":"Nat. 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