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B."],"published-print":{"date-parts":[[2016,5,11]]},"abstract":"<jats:p>Cuticular hydrocarbons (CHCs) play a critical role in the establishment of the waterproof barrier that prevents dehydration and wetting in insects. While rich data are available on CHC composition in different species, we know little about their distribution and organization. Here, we report on our studies of the surface barrier of the fruit fly<jats:italic>Drosophila melanogaster<\/jats:italic>applying a newly developed Eosin Y staining method. The inert Eosin Y penetrates different regions of the adult body at distinct temperatures. By contrast, the larval body takes up the dye rather uniformly and gradually with increasing temperature. Cooling down specimens to 25\u00b0C after incubation at higher temperatures restores impermeability. Eosin Y penetration is also sensitive to lipid solvents such as chloroform indicating that permeability depends on CHCs. As in<jats:italic>D. melanogaster<\/jats:italic>adult flies, Eosin Y penetration is regionalized in<jats:italic>Tenebrio molitor<\/jats:italic>larvae, whereas it is not in<jats:italic>Locusta migratoria<\/jats:italic>nymphs. Regionalization of the fly surface implies tissue-specific variation of the genetic or biochemical programmes of CHC production and deposition. The Eosin Y-based map of CHC distribution may serve to identify the respective factors that are activated to accommodate ecological needs.<\/jats:p>","DOI":"10.1098\/rspb.2015.2994","type":"journal-article","created":{"date-parts":[[2016,5,11]],"date-time":"2016-05-11T09:14:00Z","timestamp":1462958040000},"page":"20152994","update-policy":"https:\/\/doi.org\/10.1098\/crossmark-policy","source":"Crossref","is-referenced-by-count":44,"title":["Regionalization of surface lipids in insects"],"prefix":"10.1098","volume":"283","author":[{"given":"Yiwen","family":"Wang","sequence":"first","affiliation":[{"name":"Animal Genetics, Eberhard-Karls University of T\u00fcbingen, Auf der Morgenstelle 15, T\u00fcbingen 72076, Germany"},{"name":"Robert-Bosch Krankenhaus, Institut f\u00fcr Klinische Pharmakologie, Auerbachstrasse 112, Stuttgart 70376, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhitao","family":"Yu","sequence":"additional","affiliation":[{"name":"Institute of Applied Biology, College of Life Science, Shanxi University, Taiyuan, Shanxi 030006, People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jianzhen","family":"Zhang","sequence":"additional","affiliation":[{"name":"Institute of Applied Biology, College of Life Science, Shanxi University, Taiyuan, Shanxi 030006, People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bernard","family":"Moussian","sequence":"additional","affiliation":[{"name":"Applied Zoology, Technical University Dresden, Zellescher Weg 20b, Dresden 01217, Germany"},{"name":"iBV, Universit\u00e9 Nice Sophia-Antipolis, Parc Valrose, Nice 06108, France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"175","published-online":{"date-parts":[[2016,5,11]]},"reference":[{"key":"e_1_3_6_2_2","doi-asserted-by":"publisher","DOI":"10.1017\/CBO9780511711909"},{"key":"e_1_3_6_3_2","doi-asserted-by":"publisher","DOI":"10.1002\/arch.940060404"},{"key":"e_1_3_6_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/0305-0491(88)90305-7"},{"key":"e_1_3_6_5_2","doi-asserted-by":"publisher","DOI":"10.1093\/icb\/38.3.471"},{"key":"e_1_3_6_6_2","doi-asserted-by":"publisher","DOI":"10.1673\/031.001.0401"},{"key":"e_1_3_6_7_2","doi-asserted-by":"crossref","first-page":"373","DOI":"10.1242\/jeb.12.4.373","article-title":"The evaporation of water from the cockroach","volume":"12","author":"Ramsey JA","year":"1935","journal-title":"J. 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