{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T15:43:20Z","timestamp":1790783000206,"version":"4.1.0"},"reference-count":71,"publisher":"The Royal Society","issue":"1934","license":[{"start":{"date-parts":[[2020,9,2]],"date-time":"2020-09-02T00:00:00Z","timestamp":1599004800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2020,9,2]],"date-time":"2020-09-02T00:00:00Z","timestamp":1599004800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001665","name":"Agence Nationale de la Recherche","doi-asserted-by":"crossref","award":["ANR-11-LABX-0028"],"award-info":[{"award-number":["ANR-11-LABX-0028"]}],"id":[{"id":"10.13039\/501100001665","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["royalsocietypublishing.org"],"crossmark-restriction":true},"short-container-title":["Proc. R. Soc. B."],"published-print":{"date-parts":[[2020,9,9]]},"abstract":"<jats:p>\n            Legumes can meet their nitrogen requirements through root nodule symbiosis, which could also trigger plant systemic resistance against pests. The pea aphid\n            <jats:italic>Acyrthosiphon pisum<\/jats:italic>\n            , a legume pest, can harbour different facultative symbionts (FS) influencing various traits of their hosts. It is therefore worth determining if and how the symbionts of the plant and the aphid modulate their interaction. We used different pea aphid lines without FS or with a single one (\n            <jats:italic>Hamiltonella defensa<\/jats:italic>\n            ,\n            <jats:italic>Regiella insecticola, Serratia symbiotica<\/jats:italic>\n            ) to infest\n            <jats:italic>Medicago truncatula<\/jats:italic>\n            plants inoculated with\n            <jats:italic>Sinorhizobium meliloti<\/jats:italic>\n            (symbiotic nitrogen fixation, SNF) or supplemented with nitrate (non-inoculated, NI). The growth of SNF and NI plants was reduced by aphid infestation, while aphid weight (but not survival) was lowered on SNF compared to NI plants. Aphids strongly affected the plant nitrogen fixation depending on their symbiotic status, suggesting indirect relationships between aphid- and plant-associated microbes. Finally, all aphid lines triggered expression of\n            <jats:italic>Pathogenesis-Related Protein 1<\/jats:italic>\n            (\n            <jats:italic>PR1<\/jats:italic>\n            ) and\n            <jats:italic>Proteinase Inhibitor (PI)<\/jats:italic>\n            , respective markers for salicylic and jasmonic pathways, in SNF plants, compared to only\n            <jats:italic>PR1<\/jats:italic>\n            in NI plants. We demonstrate that the plant symbiotic status influences plant\u2013aphid interactions while that of the aphid can modulate the amplitude of the plant's defence response.\n          <\/jats:p>","DOI":"10.1098\/rspb.2020.1493","type":"journal-article","created":{"date-parts":[[2020,9,2]],"date-time":"2020-09-02T07:25:36Z","timestamp":1599031536000},"page":"20201493","update-policy":"https:\/\/doi.org\/10.1098\/crossmark-policy","source":"Crossref","is-referenced-by-count":17,"title":["Aphid infestation differently affects the defences of nitrate-fed and nitrogen-fixing\n            <i>Medicago truncatula<\/i>\n            and alters symbiotic nitrogen fixation"],"prefix":"10.1098","volume":"287","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4318-5549","authenticated-orcid":true,"given":"Gaurav","family":"Pandharikar","sequence":"first","affiliation":[{"name":"Universit\u00e9 C\u00f4te d'Azur, INRAE, CNRS, ISA, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7683-718X","authenticated-orcid":true,"given":"Jean-Luc","family":"Gatti","sequence":"additional","affiliation":[{"name":"Universit\u00e9 C\u00f4te d'Azur, INRAE, CNRS, ISA, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0620-5835","authenticated-orcid":true,"given":"Jean-Christophe","family":"Simon","sequence":"additional","affiliation":[{"name":"INRAE, UMR IGEPP, Domaine de la Motte, Le Rheu, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4578-3366","authenticated-orcid":true,"given":"Pierre","family":"Frendo","sequence":"additional","affiliation":[{"name":"Universit\u00e9 C\u00f4te d'Azur, INRAE, CNRS, ISA, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3349-6925","authenticated-orcid":true,"given":"Maryl\u00e8ne","family":"Poiri\u00e9","sequence":"additional","affiliation":[{"name":"Universit\u00e9 C\u00f4te d'Azur, INRAE, CNRS, ISA, France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"175","published-online":{"date-parts":[[2020,9,2]]},"reference":[{"key":"e_1_3_6_2_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-genet-110410-132549"},{"key":"e_1_3_6_3_2","doi-asserted-by":"publisher","DOI":"10.3389\/fpls.2016.00454"},{"key":"e_1_3_6_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.micres.2013.09.012"},{"key":"e_1_3_6_5_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF00032245"},{"key":"e_1_3_6_6_2","doi-asserted-by":"publisher","DOI":"10.1093\/jxb\/eru435"},{"key":"e_1_3_6_7_2","doi-asserted-by":"publisher","DOI":"10.3389\/fpls.2013.00081"},{"key":"e_1_3_6_8_2","first-page":"1","article-title":"Cross-talk between plant defence signalling pathways: boost or burden?","volume":"3","author":"Pieterse CMJ","year":"2001","journal-title":"AgBiotechNet"},{"key":"e_1_3_6_9_2","doi-asserted-by":"publisher","DOI":"10.1111\/1365-2435.12100"},{"key":"e_1_3_6_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.crvi.2010.03.009"},{"key":"e_1_3_6_11_2","doi-asserted-by":"publisher","DOI":"10.1080\/17429140802567173"},{"key":"e_1_3_6_12_2","doi-asserted-by":"publisher","DOI":"10.1098\/rspb.1993.0098"},{"key":"e_1_3_6_13_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.crvi.2009.09.007"},{"key":"e_1_3_6_14_2","volume-title":"Endosymbiosis of animals with plant microorganisms","author":"Buchner P","year":"1965"},{"key":"e_1_3_6_15_2","doi-asserted-by":"publisher","DOI":"10.1186\/s40168-018-0562-9"},{"key":"e_1_3_6_16_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.aspen.2017.03.025"},{"key":"e_1_3_6_17_2","doi-asserted-by":"publisher","DOI":"10.1098\/rsbl.2011.0249"},{"key":"e_1_3_6_18_2","doi-asserted-by":"publisher","DOI":"10.1007\/s10682-016-9856-1"},{"key":"e_1_3_6_19_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-ento-112408-085305"},{"key":"e_1_3_6_20_2","doi-asserted-by":"publisher","DOI":"10.1098\/rspb.2003.2430"},{"key":"e_1_3_6_21_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0811117106"},{"key":"e_1_3_6_22_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cub.2013.07.029"},{"key":"e_1_3_6_23_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cois.2017.12.009"},{"key":"e_1_3_6_24_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1094611"},{"key":"e_1_3_6_25_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00709-010-0156-2"},{"key":"e_1_3_6_26_2","doi-asserted-by":"publisher","DOI":"10.1111\/tpj.13773"},{"key":"e_1_3_6_27_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0021831"},{"key":"e_1_3_6_28_2","doi-asserted-by":"publisher","DOI":"10.1006\/pmpp.1999.0213"},{"key":"e_1_3_6_29_2","doi-asserted-by":"publisher","DOI":"10.2307\/3869566"},{"key":"e_1_3_6_30_2","doi-asserted-by":"publisher","DOI":"10.1104\/pp.107.111971"},{"key":"e_1_3_6_31_2","unstructured":"Barker D et al. 2006 Growing M. truncatula : choice of substrates and growth conditions. 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