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R. Soc. B."],"published-print":{"date-parts":[[2020,12,23]]},"abstract":"<jats:p>\n            Many lakes across Canada and northern Europe have experienced declines in ambient phosphorus (P) and calcium (Ca) supply for over 20 years. While these declines might create or exacerbate nutrient limitation in aquatic food webs, our ability to detect and quantify different types of nutrient stress on zooplankton remains rudimentary. Here, we used growth bioassay experiments and whole transcriptome RNAseq, collectively nutrigenomics, to examine the nutritional phenotypes produced by low supplies of P and Ca separately and together in the freshwater zooplankter\n            <jats:italic>Daphnia pulex<\/jats:italic>\n            . We found that daphniids in all three nutrient-deficient categories grew slower and differed in their elemental composition. Our RNAseq results show distinct responses in singly limited treatments (Ca or P) and largely a mix of these responses in animals under low Ca and P conditions. Deeper investigation of effect magnitude and gene functional annotations reveals this patchwork of responses to cumulatively represent a co-limited nutritional phenotype. Linear discriminant analysis identified a significant separation between nutritional treatments based upon gene expression patterns with the expression patterns of just five genes needed to predict animal nutritional status with 99% accuracy. These data reveal how nutritional phenotypes are altered by individual and co-limitation of two highly important nutritional elements (Ca and P) and provide evidence that aquatic consumers can respond to limitation by more than one nutrient at a time by differentially altering their metabolism. This use of nutrigenomics demonstrates its potential to address many of the inherent complexities in studying interactions between multiple nutritional stressors in ecology and beyond.\n          <\/jats:p>","DOI":"10.1098\/rspb.2020.2302","type":"journal-article","created":{"date-parts":[[2020,12,23]],"date-time":"2020-12-23T00:06:45Z","timestamp":1608682005000},"page":"20202302","update-policy":"https:\/\/doi.org\/10.1098\/crossmark-policy","source":"Crossref","is-referenced-by-count":4,"title":["The complexity of co-limitation: nutrigenomics reveal non-additive interactions of calcium and phosphorus on gene expression in\n            <i>Daphnia pulex<\/i>"],"prefix":"10.1098","volume":"287","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0225-6371","authenticated-orcid":true,"given":"Catriona L. 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