{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,13]],"date-time":"2026-01-13T15:52:14Z","timestamp":1768319534289,"version":"3.49.0"},"reference-count":58,"publisher":"The Royal Society","issue":"2025","license":[{"start":{"date-parts":[[2024,6,1]],"date-time":"2024-06-01T00:00:00Z","timestamp":1717200000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2024,6,1]],"date-time":"2024-06-01T00:00:00Z","timestamp":1717200000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100000038","name":"Natural Sciences and Engineering Research Council of Canada","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100000038","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["royalsocietypublishing.org"],"crossmark-restriction":true},"short-container-title":["Proc. R. Soc. B."],"published-print":{"date-parts":[[2024,6]]},"abstract":"<jats:p>\n            Interspecies hybrid sterility has been extensively studied, especially in the genus\n            <jats:italic>Drosophila<\/jats:italic>\n            . Hybrid sterility is more often found in the heterogametic (XY or ZW) sex, a trend called Haldane\u2019s rule. Although this phenomenon is pervasive, identification of a common genetic mechanism remains elusive, with modest support found for a range of potential theories. Here, we identify a single precise morphological phenotype, which we call \u2018needle-eye sperm\u2019, that is associated with hybrid sterility in three separate species pairs that span the\n            <jats:italic>Drosophila<\/jats:italic>\n            genus. The nature of the phenotype indicates a common point of meiotic failure in sterile hybrid males. We used 10 generations of backcross selection paired with whole-genome pooled sequencing to genetically map the regions underlying the needle-eye (NE) sperm phenotype. Surprisingly, the sterility phenotype was present in ~50% of males even after 10 generations of backcrossing, and only a single region of the X chromosome was associated with sterility in one direction of backcross. Owing to the common phenotype among sterile male hybrids\n            <jats:italic>,<\/jats:italic>\n            and the strong effect of individual loci, further exploration of these findings may identify a universal mechanism for the evolution of hybrid sterility.\n          <\/jats:p>","DOI":"10.1098\/rspb.2024.0483","type":"journal-article","created":{"date-parts":[[2024,6,18]],"date-time":"2024-06-18T23:07:33Z","timestamp":1718752053000},"update-policy":"https:\/\/doi.org\/10.1098\/crossmark-policy","source":"Crossref","is-referenced-by-count":1,"title":["Identification and genetic analysis of a pervasive \u2018needle-eye\u2019 sperm phenotype in\n            <i>Drosophila<\/i>\n            sterile hybrid males"],"prefix":"10.1098","volume":"291","author":[{"given":"Rachelle L.","family":"Kanippayoor","sequence":"first","affiliation":[{"name":"Department of Biology, Western University, London, Ontario N6A 5B7, Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Charles","family":"Soeder","sequence":"additional","affiliation":[{"name":"Department of Biology, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tom","family":"Hsiang","sequence":"additional","affiliation":[{"name":"School of Environmental Sciences, University of Guelph, Guelph, Ontario N1G 2W1, Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Corbin D.","family":"Jones","sequence":"additional","affiliation":[{"name":"Department of Biology, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8088-4007","authenticated-orcid":true,"given":"Amanda J.","family":"Moehring","sequence":"additional","affiliation":[{"name":"Department of Biology, Western University, London, Ontario N6A 5B7, Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"175","published-online":{"date-parts":[[2024,6,19]]},"reference":[{"key":"e_1_3_8_2_2","volume-title":"Speciation","author":"Coyne JA","year":"2004","unstructured":"Coyne JA, Orr HA. 2004 Speciation. 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