{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,27]],"date-time":"2026-06-27T07:26:12Z","timestamp":1782545172193,"version":"3.54.5"},"reference-count":46,"publisher":"The Royal Society","issue":"2034","license":[{"start":{"date-parts":[[2024,11,1]],"date-time":"2024-11-01T00:00:00Z","timestamp":1730419200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2024,11,1]],"date-time":"2024-11-01T00:00:00Z","timestamp":1730419200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000057","name":"National Institute of General Medical Sciences","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100000057","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000185","name":"Defense Advanced Research Projects Agency","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100000185","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Iraqi Ministry of Higher Education and Scientific Research"},{"name":"School of Biomedical Sciences, University of Plymouth, UK"}],"content-domain":{"domain":["royalsocietypublishing.org"],"crossmark-restriction":true},"short-container-title":["Proc. R. Soc. B."],"published-print":{"date-parts":[[2024,11]]},"abstract":"<jats:p>\n                    Spillover of infectious diseases from wild animal populations constitutes a long-standing threat to human health for which few globally viable solutions have been developed. The use of oral baits laden with conventional vaccines distributed\n                    <jats:italic>en masse<\/jats:italic>\n                    represents one success story but is costly and practicable primarily for rabies risk reduction in North American and European carnivores. Efforts to expand vaccination to control pathogens within less accessible wildlife populations have raised interest in a new kind of vaccine capable of spreading pathogen-specific immunity through autonomous spread. However, such \u2018transmissible\u2019 vaccines raise concerns about the irrevocable release of genetically modified viruses into the environment. Herein, we explore the feasibility of an intrinsic strategy for transgene control within these vaccines based on the genetic destabilizing effect of\n                    <jats:italic>cis<\/jats:italic>\n                    -acting sequences flanking the heterologous transgene of interest. While suitable for the control of transgene stability within all types of DNA-viral vectored vaccines, this strategy has particular applicability to transmissible vaccines. Using a combination of experiments, mathematical modelling and whole-genome sequencing, we show that the rate of transgene loss can be controlled by varying the lengths of the direct repeat sequences. This opens a way for fine-tuning the lifespan of a transmissible vaccine in the wild.\n                  <\/jats:p>","DOI":"10.1098\/rspb.2024.1827","type":"journal-article","created":{"date-parts":[[2024,11,12]],"date-time":"2024-11-12T19:07:17Z","timestamp":1731438437000},"update-policy":"https:\/\/doi.org\/10.1098\/crossmark-policy","source":"Crossref","is-referenced-by-count":2,"title":["Fine-tuning the evolutionary stability of recombinant herpesviral transmissible vaccines"],"prefix":"10.1098","volume":"291","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0788-9503","authenticated-orcid":true,"given":"Baca","family":"Chan","sequence":"first","affiliation":[{"name":"Institute for Respiratory Health, University of Western Australia, Nedlands, WA 6009, Australia"},{"name":"School of Biomedical Science, University of Western Australia, Nedlands, WA 6009, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9817-0056","authenticated-orcid":true,"given":"Scott L.","family":"Nuismer","sequence":"additional","affiliation":[{"name":"Department of Biological Sciences, University of Idaho, Moscow, ID 83844, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hujaz","family":"Alqirbi","sequence":"additional","affiliation":[{"name":"School of Biomedical Sciences, University of Plymouth, Plymouth PL4 8AA, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0093-7170","authenticated-orcid":true,"given":"Jenna","family":"Nichols","sequence":"additional","affiliation":[{"name":"MRC-University of Glasgow Centre for Virus Research, Glasgow G61 1QH, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1179-9041","authenticated-orcid":true,"given":"Christopher H.","family":"Remien","sequence":"additional","affiliation":[{"name":"Department of Mathematics and Statistical Science, University of Idaho, Moscow, ID 83844, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4991-9128","authenticated-orcid":true,"given":"Andrew J.","family":"Davison","sequence":"additional","affiliation":[{"name":"MRC-University of Glasgow Centre for Virus Research, Glasgow G61 1QH, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0124-4061","authenticated-orcid":true,"given":"Michael A.","family":"Jarvis","sequence":"additional","affiliation":[{"name":"School of Biomedical Sciences, University of Plymouth, Plymouth PL4 8AA, UK"},{"name":"The Vaccine Group, Plymouth, Devon PL6 6BU, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8601-8292","authenticated-orcid":true,"given":"Alec J.","family":"Redwood","sequence":"additional","affiliation":[{"name":"Institute for Respiratory Health, University of Western Australia, Nedlands, WA 6009, Australia"},{"name":"School of Biomedical Science, University of Western Australia, Nedlands, WA 6009, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"175","published-online":{"date-parts":[[2024,11,13]]},"reference":[{"key":"e_1_3_8_2_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature06536"},{"key":"e_1_3_8_3_2","doi-asserted-by":"publisher","DOI":"10.1098\/rstb.2001.0888"},{"key":"e_1_3_8_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.econmod.2022.106147"},{"key":"e_1_3_8_5_2","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.69336"},{"key":"e_1_3_8_6_2","doi-asserted-by":"publisher","DOI":"10.1126\/sciadv.abl4183"},{"key":"e_1_3_8_7_2","doi-asserted-by":"publisher","DOI":"10.1126\/sciadv.ade6169"},{"key":"e_1_3_8_8_2","doi-asserted-by":"publisher","DOI":"10.1637\/10183-041012-Review.1"},{"key":"e_1_3_8_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0264-410X(02)00778-8"},{"key":"e_1_3_8_10_2","doi-asserted-by":"publisher","DOI":"10.1038\/srep18232"},{"key":"e_1_3_8_11_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0609122104"},{"key":"e_1_3_8_12_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pbio.1000053"},{"key":"e_1_3_8_13_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0002372"},{"key":"e_1_3_8_14_2","doi-asserted-by":"publisher","DOI":"10.1098\/rstb.2012.0142"},{"key":"e_1_3_8_15_2","doi-asserted-by":"publisher","DOI":"10.1186\/s13567-017-0459-9"},{"key":"e_1_3_8_16_2","doi-asserted-by":"publisher","DOI":"10.1038\/354520a0"},{"key":"e_1_3_8_17_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.tim.2017.09.007"},{"key":"e_1_3_8_18_2","doi-asserted-by":"publisher","DOI":"10.1098\/rspb.2016.1903"},{"key":"e_1_3_8_19_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41559-020-1254-y"},{"key":"e_1_3_8_20_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0264-410X(01)00184-0"},{"key":"e_1_3_8_21_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.2216667120"},{"key":"e_1_3_8_22_2","doi-asserted-by":"publisher","DOI":"10.1586\/14760584.2016.1106942"},{"key":"e_1_3_8_23_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.pt.2020.08.006"},{"key":"e_1_3_8_24_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.abj5593"},{"key":"e_1_3_8_25_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.abo0238"},{"key":"e_1_3_8_26_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.adn3231"},{"key":"e_1_3_8_27_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00430-019-00597-7"},{"key":"e_1_3_8_28_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41541-022-00503-y"},{"key":"e_1_3_8_29_2","doi-asserted-by":"publisher","DOI":"10.1111\/eva.12806"},{"key":"e_1_3_8_30_2","doi-asserted-by":"publisher","DOI":"10.1071\/WR07041"},{"key":"e_1_3_8_31_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1439-0531.2012.02079.x"},{"key":"e_1_3_8_32_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41392-021-00487-6"},{"key":"e_1_3_8_33_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.2108610119"},{"key":"e_1_3_8_34_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.79.5.2998-3008.2005"},{"key":"e_1_3_8_35_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.76.2.885-894.2002"},{"key":"e_1_3_8_36_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.78.14.7536-7544.2004"},{"key":"e_1_3_8_37_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.83.14.5199"},{"key":"e_1_3_8_38_2","doi-asserted-by":"publisher","DOI":"10.1093\/synbio\/ysab018"},{"key":"e_1_3_8_39_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.00545-11"},{"key":"e_1_3_8_40_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.01456-20"},{"key":"e_1_3_8_41_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.00160-08"},{"key":"e_1_3_8_42_2","doi-asserted-by":"publisher","DOI":"10.1128\/jvi.69.10.6098-6105.1995"},{"key":"e_1_3_8_43_2","doi-asserted-by":"publisher","DOI":"10.1099\/jgv.0.001873"},{"key":"e_1_3_8_44_2","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.1923"},{"key":"e_1_3_8_45_2","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btp352"},{"key":"e_1_3_8_46_2","doi-asserted-by":"publisher","DOI":"10.1093\/bib\/bbs012"},{"key":"e_1_3_8_47_2","unstructured":"Chan 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