{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,2]],"date-time":"2026-06-02T14:31:57Z","timestamp":1780410717936,"version":"3.54.1"},"reference-count":45,"publisher":"The Royal Society","issue":"2058","license":[{"start":{"date-parts":[[2025,11,1]],"date-time":"2025-11-01T00:00:00Z","timestamp":1761955200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/royalsociety.org\/-\/media\/journals\/author\/Licence-to-Publish-20062019-final.pdf"},{"start":{"date-parts":[[2025,11,1]],"date-time":"2025-11-01T00:00:00Z","timestamp":1761955200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/royalsociety.org\/journals\/ethics-policies\/data-sharing-mining\/"}],"funder":[{"name":"NIH"},{"name":"NSF"},{"DOI":"10.13039\/501100003130","name":"Fonds Wetenschappelijk Onderzoek","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100003130","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Whitman"}],"content-domain":{"domain":["royalsocietypublishing.org"],"crossmark-restriction":true},"short-container-title":["Proc. R. Soc. B."],"published-print":{"date-parts":[[2025,11]]},"abstract":"<jats:p>\n                    Bacterial endosymbionts are common in terrestrial arthropods, where they can manipulate host biology and reproduction, with\n                    <jats:italic toggle=\"yes\">Wolbachia<\/jats:italic>\n                    and\n                    <jats:italic toggle=\"yes\">Cardinium<\/jats:italic>\n                    as the best-known examples. Vertical transmission is a hallmark of such endosymbiosis. The high incidence of parthenogenesis makes non-marine ostracods (small, bivalved crustaceans) excellent models for exploring the potential effects of endosymbiosis on host reproduction in fully aquatic arthropods. Here, we complement DNA sequencing-based detection of\n                    <jats:italic toggle=\"yes\">Cardinium<\/jats:italic>\n                    in non-marine ostracods with an imaging approach based on microdissection and fluorescence\n                    <jats:italic toggle=\"yes\">in situ<\/jats:italic>\n                    hybridization (FISH). We use confocal microscopy to illustrate the location and spatial organization of\n                    <jats:italic toggle=\"yes\">Cardinium<\/jats:italic>\n                    cells within female ovaries and eggs in natural, parthenogenetic populations of three species of ostracods with mixed reproduction. Our results provide evidence that these bacteria are stable endosymbionts and confirm vertical transmission of\n                    <jats:italic toggle=\"yes\">Cardinium<\/jats:italic>\n                    in non-marine ostracods. The location of\n                    <jats:italic toggle=\"yes\">Cardinium<\/jats:italic>\n                    in reproductive tissues of parthenogenetic females further indicates that these intracellular bacteria are potential reproductive manipulators in at least some non-marine ostracod species. Given that our knowledge on endosymbionts is still largely biased towards terrestrial arthropods with haplodiploidy, our results open the way to novel comparative studies of reproductive manipulation by endosymbionts in a much wider range of taxa.\n                  <\/jats:p>","DOI":"10.1098\/rspb.2025.1193","type":"journal-article","created":{"date-parts":[[2025,11,12]],"date-time":"2025-11-12T00:06:13Z","timestamp":1762905973000},"update-policy":"https:\/\/doi.org\/10.1098\/crossmark-policy","source":"Crossref","is-referenced-by-count":1,"title":["Vertical transmission of\n                    <i>Cardinium<\/i>\n                    bacteria in parthenogenetic non-marine ostracods (Crustacea)"],"prefix":"10.1098","volume":"292","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9269-6487","authenticated-orcid":true,"given":"Isa","family":"Sch\u00f6n","sequence":"first","affiliation":[{"name":"Department of Freshwater Biology, Royal Belgian Institute of Natural Sciences","place":["1000 Brussels, Belgium"]},{"name":"Centre for Environmental Sciences, Hasselt University - Diepenbeek Campus","place":["3590 Diepenbeek, Belgium"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9024-8311","authenticated-orcid":true,"given":"Scott","family":"Chimileski","sequence":"additional","affiliation":[{"name":"Bay Paul Center, Marine Biological Laboratory, Woods Hole, MA 02543, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0696-2334","authenticated-orcid":true,"given":"Jessica","family":"Mark Welch","sequence":"additional","affiliation":[{"name":"Bay Paul Center, Marine Biological Laboratory, Woods Hole, MA 02543, USA"},{"name":"The ADA Forsyth Institute, Somerville, MA 02143, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8680-973X","authenticated-orcid":true,"given":"Koen","family":"Martens","sequence":"additional","affiliation":[{"name":"Department of Freshwater Biology, Royal Belgian Institute of Natural Sciences","place":["1000 Brussels, Belgium"]},{"name":"Department of Biology, Ghent University","place":["9000 Ghent, Belgium"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"175","published-online":{"date-parts":[[2025,11,12]]},"reference":[{"key":"e_1_3_8_2_2","first-page":"329","article-title":"Studies on Rickettsia-like micro-organisms in insects","volume":"44","author":"Hertig M","year":"1924","unstructured":"Hertig M, Wolbach SB. 1924 Studies on Rickettsia-like micro-organisms in insects. J. Med. Res. 44, 329\u2013374.","journal-title":"J. Med. Res."},{"key":"e_1_3_8_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/0035-9203(75)90110-8"},{"key":"e_1_3_8_4_2","doi-asserted-by":"publisher","DOI":"10.1098\/rsbl.2019.0273"},{"key":"e_1_3_8_5_2","doi-asserted-by":"publisher","DOI":"10.1242\/jeb.220079"},{"key":"e_1_3_8_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cois.2023.101023"},{"key":"e_1_3_8_7_2","doi-asserted-by":"publisher","DOI":"10.1111\/jeb.13069"},{"key":"e_1_3_8_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.tig.2011.05.002"},{"key":"e_1_3_8_9_2","doi-asserted-by":"publisher","DOI":"10.1186\/s12866-019-1638-9"},{"key":"e_1_3_8_10_2","doi-asserted-by":"publisher","DOI":"10.1007\/s10493-006-9019-0"},{"key":"e_1_3_8_11_2","doi-asserted-by":"publisher","DOI":"10.1096\/fj.07-101162"},{"key":"e_1_3_8_12_2","doi-asserted-by":"publisher","DOI":"10.1007\/s002849900270"},{"key":"e_1_3_8_13_2","doi-asserted-by":"publisher","DOI":"10.1016\/B978-0-12-385026-3.00030-9"},{"key":"e_1_3_8_14_2","doi-asserted-by":"publisher","DOI":"10.1016\/0169-5347(94)90127-9"},{"key":"e_1_3_8_15_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2540.1998.00454.x"},{"key":"e_1_3_8_16_2","doi-asserted-by":"publisher","DOI":"10.5852\/ejt.2024.974.2767"},{"key":"e_1_3_8_17_2","first-page":"119","volume-title":"Sex and parthenogenesis: evolutionary ecology of reproductive modes in non-marine ostracods","author":"Vandekerckhove T","year":"1998","unstructured":"Vandekerckhove T. 1998 Cytoplasmatic bacteria in Heterocypris incongruens (Crustacea, Ostracoda). In Sex and parthenogenesis: evolutionary ecology of reproductive modes in non-marine ostracods (ed. K Martens), pp. 119\u2013125. Leiden, The Netherlands: Backhuys."},{"key":"e_1_3_8_18_2","doi-asserted-by":"publisher","DOI":"10.7717\/peerj.6039"},{"key":"e_1_3_8_19_2","doi-asserted-by":"publisher","DOI":"10.1093\/jcbiol\/ruz018"},{"key":"e_1_3_8_20_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ympev.2018.09.008"},{"key":"e_1_3_8_21_2","doi-asserted-by":"publisher","DOI":"10.1007\/s10750-019-04110-2"},{"key":"e_1_3_8_22_2","doi-asserted-by":"publisher","DOI":"10.2108\/zs200127"},{"key":"e_1_3_8_23_2","article-title":"A new genus and species of the spring-endemic ostracoda (Cypricercinae, Cyprididae) and its genetic population structure among rheocrenic springs in Japan","volume":"63","author":"Munakata M","year":"2024","unstructured":"Munakata M, Tanaka H, Kakui K. 2024 A new genus and species of the spring-endemic ostracoda (Cypricercinae, Cyprididae) and its genetic population structure among rheocrenic springs in Japan. Zool. Stud. 63, 51. (doi:10.6620\/ZS.2024.63-51)","journal-title":"Zool. 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