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Diversity of such processes usually arises through the addition or loss of components rather than through changes in their order, since reordering can disrupt functional integration. Here, we show that reordering of the steps associated with termite nuptial pairing can generate behavioural diversity. In most termites, dispersed adults shed their wings before initiating tandem pairing, as wings interfere with communication between females and males. However, we found that, in the Australian termite Microcerotermes nervosus, tandem running occurs prior to wing shedding (dealation), and the act of tandem running itself facilitates dealation. Video tracking revealed that wing removal improves movement coordination, and comparative analysis of sympatric species showed that M. nervosus exhibits much higher tandem stability than others. These results suggest that enhanced movement coordination of this species may have potentiated step reordering. Our findings demonstrate that even functionally conserved sequences can be reorganized and that overcoming such constraints can open novel pathways for behavioural diversification.<\/jats:p>","DOI":"10.1098\/rspb.2025.2738","type":"journal-article","created":{"date-parts":[[2026,3,5]],"date-time":"2026-03-05T14:10:47Z","timestamp":1772719847000},"source":"Crossref","is-referenced-by-count":0,"title":["Diversification of pre-mating behaviours through temporal reordering of components"],"prefix":"10.1098","volume":"293","author":[{"ORCID":"https:\/\/orcid.org\/0009-0001-8981-5024","authenticated-orcid":false,"given":"Elijah P.","family":"Carroll","sequence":"first","affiliation":[{"name":"Auburn University 1 , , ,","place":["Auburn, AL 36849, USA"],"department":["Department of Entomology and Plant Pathology"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Taisuke","family":"Kanao","sequence":"additional","affiliation":[{"name":"Yamagata University 2 , ,","place":["Yamagata 990-8560, Japan"],"department":["Faculty of Science"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nathan","family":"Lo","sequence":"additional","affiliation":[{"name":"The University of Sydney 3 , , ,","place":["Sydney, New South Wales 2006, Australia"],"department":["School of Life and Environmental Sciences"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6731-8684","authenticated-orcid":false,"given":"Nobuaki","family":"Mizumoto","sequence":"additional","affiliation":[{"name":"Auburn University 1 , , ,","place":["Auburn, AL 36849, USA"],"department":["Department of Entomology and Plant Pathology"]},{"name":"Okinawa Institute of Science and Technology 4 , , ,","place":["Onna, Okinawa 904-0495, Japan"],"department":["Evolutionary Genomics Unit"]},{"name":"Okinawa Institute of Science and Technology 5 , , ,","place":["Onna, Okinawa, Japan, 904-0495"],"department":["Computational Neuroethology Unit"]}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"175","published-online":{"date-parts":[[2026,3,4]]},"reference":[{"key":"2026030509104096700_R1","doi-asserted-by":"crossref","DOI":"10.7554\/eLife.50371","article-title":"Temporal cascade of frontal, motor and muscle processes underlying human action-stopping","volume":"9","author":"Jana","year":"2020","journal-title":"eLife"},{"key":"2026030509104096700_R2","doi-asserted-by":"crossref","first-page":"4983","DOI":"10.1016\/j.cub.2021.09.030","article-title":"Distinct movement patterns generate stages of spider web building","volume":"31","author":"Corver","year":"2021","journal-title":"Curr. 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