{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,16]],"date-time":"2026-06-16T23:26:33Z","timestamp":1781652393835,"version":"3.54.5"},"reference-count":72,"publisher":"The Royal Society","issue":"2072","license":[{"start":{"date-parts":[[2026,6,10]],"date-time":"2026-06-10T00:00:00Z","timestamp":1781049600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,6,10]],"date-time":"2026-06-10T00:00:00Z","timestamp":1781049600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100000270","name":"Natural Environment Research Council","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100000270","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000643","name":"Daphne Jackson Trust","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100000643","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Royal Society Dorothy Hodgkin Fellowship"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,6,10]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>When females mate with multiple males, ejaculates from different partners compete within the female reproductive tract. Males gain a competitive advantage by transferring seminal fluid proteins (Sfps) that manipulate female post-mating responses, such as reducing female propensity to remate. But in monandrous species, where females typically mate only once, post-mating sexual selection is absent, raising questions about the complexity and identity of Sfps in monandrous seminal proteomes. To address this, we characterized the seminal proteome of the monandrous fly Drosophila subobscura. Using a label-free quantitative proteomics approach, we identified 172 Sfps, comparable in number to the 153 Sfps previously reported in polyandrous Drosophila melanogaster using a similar approach. The D. subobscura seminal proteome contains all the D. melanogaster sex peptide network\u2014proteins that control the female post-mating response\u2014and many proteins known to be involved in post-copulatory sexual selection. We also found a surprising over-abundance of proteins previously shown to be downregulated under monogamy selection in D. melanogaster. Collectively, the patterns suggest that the D. subobscura seminal proteome is characterized more by conflict than cooperation and, despite differences in mating systems, is compositionally similar to that of D. melanogaster, with a complex proteome rich in receptivity-inhibiting Sfps.<\/jats:p>","DOI":"10.1098\/rspb.2026.0345","type":"journal-article","created":{"date-parts":[[2026,6,10]],"date-time":"2026-06-10T00:16:11Z","timestamp":1781050571000},"source":"Crossref","is-referenced-by-count":1,"title":["The seminal proteome of a monandrous fly,\n                    <i>Drosophila subobscura<\/i>"],"prefix":"10.1098","volume":"293","author":[{"ORCID":"https:\/\/orcid.org\/0009-0006-0001-2492","authenticated-orcid":false,"given":"Rebecca","family":"Dean","sequence":"first","affiliation":[{"name":"University of Liverpool 1 , ,","place":["Liverpool, UK, L69 7ZB"],"department":["Department of Evolution, Ecology and Behaviour"]},{"name":"University of Oxford 2 , ,","place":["Oxford, UK, OX1 3EL"],"department":["Department of Biology"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kawinnat","family":"Sue-ob","sequence":"additional","affiliation":[{"name":"University of Liverpool 3 , ,","place":["Liverpool, UK, L69 7ZB"],"department":["Computational Biology Facility"]},{"name":"Mahidol University 4 , ,","place":["Salaya, Thailand, 10400"],"department":["Department of Plant Science"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rudi","family":"Verspoor","sequence":"additional","affiliation":[{"name":"University of Liverpool 5 , , ,","place":["Liverpool, Merseyside, UK, L69 7BE"],"department":["Institute for Systems, Molecular and Integrative Biology"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jolanta","family":"Tanianis-Hughes","sequence":"additional","affiliation":[{"name":"University of Liverpool 1 , ,","place":["Liverpool, UK, L69 7ZB"],"department":["Department of Evolution, Ecology and Behaviour"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3228-5480","authenticated-orcid":false,"given":"Irem","family":"Sepil","sequence":"additional","affiliation":[{"name":"University of Oxford 2 , ,","place":["Oxford, UK, OX1 3EL"],"department":["Department of Biology"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tom A. R.","family":"Price","sequence":"additional","affiliation":[{"name":"University of Liverpool 1 , ,","place":["Liverpool, UK, L69 7ZB"],"department":["Department of Evolution, Ecology and Behaviour"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Margaret","family":"Hughes","sequence":"additional","affiliation":[{"name":"University of Liverpool 6 , ,","place":["Liverpool, UK, L69 7ZB"],"department":["Centre for Genomic Research"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stuart","family":"Hesketh","sequence":"additional","affiliation":[{"name":"University of Liverpool 7 , ,","place":["Liverpool, UK, L69 7ZB"],"department":["Centre for Proteome Research"]},{"name":"University of Lancashire 8 , ,","place":["Preston, UK, PR1 2HE"],"department":["School of Medicine"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stephen F.","family":"Goodwin","sequence":"additional","affiliation":[{"name":"University of Oxford 9 , ,","place":["Oxford, UK, OX1 3SR"],"department":["Centre for Neural Circuits and Behaviour"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Steve","family":"Paterson","sequence":"additional","affiliation":[{"name":"University of Liverpool 6 , ,","place":["Liverpool, UK, L69 7ZB"],"department":["Centre for Genomic Research"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Claire","family":"Eyers","sequence":"additional","affiliation":[{"name":"University of Liverpool 7 , ,","place":["Liverpool, UK, L69 7ZB"],"department":["Centre for Proteome Research"]},{"name":"University of Liverpool 10 , ,","place":["Liverpool, UK, L69 7BE"],"department":["Department of Biochemistry"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrew R.","family":"Jones","sequence":"additional","affiliation":[{"name":"University of Liverpool 3 , ,","place":["Liverpool, UK, L69 7ZB"],"department":["Computational Biology Facility"]},{"name":"University of Liverpool 11 , ,","place":["Liverpool, UK, L69 7BE"],"department":["Institute of Systems, Molecular and Integrative Biology"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2260-2948","authenticated-orcid":false,"given":"Stuart","family":"Wigby","sequence":"additional","affiliation":[{"name":"University of Liverpool 1 , ,","place":["Liverpool, UK, L69 7ZB"],"department":["Department of Evolution, Ecology and Behaviour"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"175","published-online":{"date-parts":[[2026,6,10]]},"reference":[{"key":"2026060920160469800_R1","doi-asserted-by":"crossref","first-page":"525","DOI":"10.1111\/j.1469-185X.1970.tb01176.x","article-title":"Sperm competition and its evolutionary consequences in the insects","volume":"45","author":"Parker","year":"1970","journal-title":"Biol. 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