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Naegleria aerobia, Hartmannella culbertsoni and H. rhysodes are pathogenic; H. castellanii, H. astronyxis, H. palestinensis, H. glebae, H. exundans, H. vermiformis, Schizopyrenus russelli, Didascalus thorntoni and Tetramitus rostratus are non-pathogenic. Strains of H. culbertsoni and H. rhysodes are present in Indian soils. A classification of the order Amoebida Kent into families Schizopyrenidae Singh, Hartmannellidae Volkonsky, 1931 emend. Singh, 1952, and Endamoebidae (Calkins), based on nuclear division, is proposed, and the relation of this classification to previously defined families and genera of amoebae and its bearing on phylogeny are discussed. Metronidazole and other anti-amoebic drugs are ineffective against N. aerobia and H. culbertsoni in vitro and in meningo-encephalitis in mice.<\/jats:p>","DOI":"10.1098\/rstb.1970.0063","type":"journal-article","created":{"date-parts":[[2006,12,15]],"date-time":"2006-12-15T16:31:17Z","timestamp":1166200277000},"page":"435-476","source":"Crossref","is-referenced-by-count":86,"title":["Studies on pathogenic and non-pathogenic small free-living amoebae and the bearing of nuclear division on the classification of the order Amoebida"],"prefix":"10.1098","volume":"259","author":[{"given":"B. N.","family":"Singh","sequence":"first","affiliation":[{"name":"Central Drug Research Institute, Lucknow, U. P. , India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S. R.","family":"Das","sequence":"additional","affiliation":[{"name":"Central Drug Research Institute, Lucknow, U. P. , India"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"175","reference":[{"key":"p_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1550-7408.1964.tb01774.x"},{"key":"p_2","unstructured":"Adam K. M. G. & Blewett D. A. 1968 Extraction and characterization of the DNA from Acanthamoeba castellanii. J. Protozool. ( Suppl.)15 35."},{"key":"p_3","unstructured":"Alexeieff A. 1911 Sur la division nucleaire et l'enkystement chez quelques amibes du groupe limax. C. r. Soc. Biol. Paris 70 455 534 588."},{"key":"p_4","unstructured":"Balamuth W. & Kawakami T. 1967 Antigenic relationship among selected hartmannellid amoebae and amoebo-flagellates. J. Protozool. {Suppl.) 14 31."},{"key":"p_5","doi-asserted-by":"publisher","DOI":"10.1111\/j.1550-7408.1963.tb01642.x"},{"key":"p_6","doi-asserted-by":"publisher","DOI":"10.1017\/S0031182000013044"},{"key":"p_7","unstructured":"Boeck W. G. & Stiles C. W. 1923 Studies on various intestinal parasites {especially amoebae of man). 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