{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,12]],"date-time":"2026-09-12T02:40:12Z","timestamp":1789180812966,"version":"build-2803163510"},"reference-count":40,"publisher":"The Royal Society","issue":"1616","license":[{"start":{"date-parts":[[2013,4,19]],"date-time":"2013-04-19T00:00:00Z","timestamp":1366329600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/royalsociety.org\/journals\/ethics-policies\/data-sharing-mining\/"}],"content-domain":{"domain":["royalsocietypublishing.org"],"crossmark-restriction":true},"short-container-title":["Phil. Trans. R. Soc. B"],"published-print":{"date-parts":[[2013,4,19]]},"abstract":"<jats:p>\n                    Two novel reductive dehalogenases (RDases) that are highly similar to each other but catalyse distinct dechlorination reactions were identified from\n                    <jats:italic>Dehalobacter<\/jats:italic>\n                    -containing mixed cultures. These two RDases were partially purified from crude protein extracts of anaerobic dechlorinating enrichment cultures using blue native polyacrylamide gel electrophoresis. Gel slices were assayed for dechlorinating activity, and associated proteins were identified using liquid chromatography tandem mass spectrometry with the metagenome of the parent culture as the reference database. The two RDases identified, annotated as CfrA and DcrA, share an amino acid identity of 95.2 per cent, but use different substrates: CfrA dechlorinates chloroform (CF) and 1,1,1-trichloroethane (1,1,1-TCA), but not 1,1-dichloroethane; DcrA dechlorinates 1,1-dichloroethane, but not CF or 1,1,1-TCA. These two novel RDases share no more than 40 per cent amino acid identity to any other known or putative RDases, but both have a twin-arginine motif and two iron\u2013sulfur binding motifs conserved in most RDases. Peptides specific to two putative membrane anchor proteins, annotated as CfrB and DcrB, were also detected in gel slices.\n                  <\/jats:p>","DOI":"10.1098\/rstb.2012.0318","type":"journal-article","created":{"date-parts":[[2013,3,11]],"date-time":"2013-03-11T22:15:04Z","timestamp":1363040104000},"page":"20120318","update-policy":"https:\/\/doi.org\/10.1098\/crossmark-policy","source":"Crossref","is-referenced-by-count":85,"title":["Identification of\n                    <i>Dehalobacter<\/i>\n                    reductive dehalogenases that catalyse dechlorination of chloroform, 1,1,1-trichloroethane and 1,1-dichloroethane"],"prefix":"10.1098","volume":"368","author":[{"given":"Shuiquan","family":"Tang","sequence":"first","affiliation":[{"name":"Department of Chemical Engineering and Applied Chemistry, University of Toronto, Toronto, Ontario, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elizabeth A.","family":"Edwards","sequence":"additional","affiliation":[{"name":"Department of Chemical Engineering and Applied Chemistry, University of Toronto, Toronto, Ontario, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"175","published-online":{"date-parts":[[2013,4,19]]},"reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1006\/enfo.2000.0010"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1080\/10937400290070080"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.pep.2012.08.007"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1021\/es990809f"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1007\/s002530051393"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.2166\/wst.1991.0591"},{"key":"e_1_3_2_8_2","volume-title":"The effects of cyanide and chloroform toxicity on methane fermentation","author":"Yang C-HJ","year":"1981"},{"key":"e_1_3_2_9_2","first-page":"117","article-title":"Metallic iron-enhanced biotransformation of carbon tetrachloride and chloroform under methanogenic conditions","volume":"3","author":"Weathers LJ","year":"1995","journal-title":"Bioremediation Chlorinated Solvents"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1897\/1551-5028(2000)019<2212:IOMBCA>2.3.CO;2"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0043-1354(99)00121-9"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0043-1354(02)00151-3"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1002\/Tcr.20134"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1128\/Aem.01269-06"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1111\/J.1462-2920.2009.02150.X"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1007\/s002030050577"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.02037-08"},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1074675"},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1462-2920.2011.02656.x"},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.1021\/es200480k"},{"key":"e_1_3_2_21_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.femsec.2005.03.003"},{"key":"e_1_3_2_22_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.02112-08"},{"key":"e_1_3_2_23_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.07325-11"},{"key":"e_1_3_2_24_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.micro.58.030603.123600"},{"key":"e_1_3_2_25_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pgen.1000714"},{"key":"e_1_3_2_26_2","doi-asserted-by":"publisher","DOI":"10.1038\/nbt1131"},{"key":"e_1_3_2_27_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.01873-12"},{"key":"e_1_3_2_28_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.01649-07"},{"key":"e_1_3_2_29_2","volume-title":"Geneious v. 5.4.2","author":"Drummond AJ","year":"2011"},{"key":"e_1_3_2_30_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0052038"},{"key":"e_1_3_2_31_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkh340"},{"key":"e_1_3_2_32_2","doi-asserted-by":"publisher","DOI":"10.1080\/10635150390235520"},{"key":"e_1_3_2_33_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0097-8485(01)00099-7"},{"key":"e_1_3_2_34_2","first-page":"203","article-title":"SIGNAL SCAN: a computer program that scans DNA sequences for eukaryotic transcriptional elements","volume":"7","author":"Prestridge DS","year":"1991","journal-title":"Comput. 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