{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,13]],"date-time":"2026-10-13T12:18:48Z","timestamp":1791893928597,"version":"4.3.4"},"reference-count":40,"publisher":"The Royal Society","issue":"1616","license":[{"start":{"date-parts":[[2013,4,19]],"date-time":"2013-04-19T00:00:00Z","timestamp":1366329600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/royalsociety.org\/journals\/ethics-policies\/data-sharing-mining\/"}],"content-domain":{"domain":["royalsocietypublishing.org"],"crossmark-restriction":true},"short-container-title":["Phil. Trans. R. Soc. B"],"published-print":{"date-parts":[[2013,4,19]]},"abstract":"<jats:p>\n                    <jats:italic>Dehalococcoides mccartyi<\/jats:italic>\n                    strains are corrinoid-auxotrophic Bacteria and axenic cultures that require vitamin B\n                    <jats:sub>12<\/jats:sub>\n                    (CN-Cbl) to conserve energy via organohalide respiration. Cultures of\n                    <jats:italic>D. mccartyi<\/jats:italic>\n                    strains BAV1, GT and FL2 grown with limiting amounts of 1 \u00b5g l\n                    <jats:sup>\u22121<\/jats:sup>\n                    CN-Cbl quickly depleted CN-Cbl, and reductive dechlorination of polychlorinated ethenes was incomplete leading to vinyl chloride (VC) accumulation. In contrast, the same cultures amended with 25 \u00b5g l\n                    <jats:sup>\u22121<\/jats:sup>\n                    CN-Cbl exhibited up to 2.3-fold higher dechlorination rates, 2.8\u20139.1-fold increased growth yields, and completely consumed growth-supporting chlorinated ethenes. To explore whether known cobamide-producing microbes supply\n                    <jats:italic>Dehalococcoides<\/jats:italic>\n                    with the required corrinoid cofactor, co-culture experiments were performed with the methanogen\n                    <jats:italic>Methanosarcina barkeri<\/jats:italic>\n                    strain Fusaro and two acetogens,\n                    <jats:italic>Sporomusa ovata<\/jats:italic>\n                    and\n                    <jats:italic>Sporomusa<\/jats:italic>\n                    sp. strain KB-1, as\n                    <jats:italic>Dehalococcoides<\/jats:italic>\n                    partner populations. During growth with H\n                    <jats:sub>2<\/jats:sub>\n                    \/CO\n                    <jats:sub>2<\/jats:sub>\n                    ,\n                    <jats:italic>M. barkeri<\/jats:italic>\n                    axenic cultures produced 4.2 \u00b1 0.1 \u00b5g l\n                    <jats:sup>\u22121<\/jats:sup>\n                    extracellular cobamide (factor III), whereas the\n                    <jats:italic>Sporomusa<\/jats:italic>\n                    cultures produced phenolyl- and\n                    <jats:italic>p<\/jats:italic>\n                    -cresolyl-cobamides. Neither factor III nor the phenolic cobamides supported\n                    <jats:italic>Dehalococcoides<\/jats:italic>\n                    reductive dechlorination activity suggesting that\n                    <jats:italic>M. barkeri<\/jats:italic>\n                    and the\n                    <jats:italic>Sporomusa<\/jats:italic>\n                    sp. cannot fulfil\n                    <jats:italic>Dehalococcoides<\/jats:italic>\n                    ' nutritional requirements.\n                    <jats:italic>Dehalococcoides<\/jats:italic>\n                    dechlorination activity and growth occurred in\n                    <jats:italic>M. barkeri<\/jats:italic>\n                    and\n                    <jats:italic>Sporomusa<\/jats:italic>\n                    sp. co-cultures amended with 10 \u00b5M 5\u2032,6\u2032-dimethylbenzimidazole (DMB), indicating that a cobalamin is a preferred corrinoid cofactor of strains BAV1, GT and FL2 when grown with chlorinated ethenes as electron acceptors. Even though the methanogen and acetogen populations tested did not produce cobalamin, the addition of DMB enabled guided biosynthesis and generated a cobalamin that supported\n                    <jats:italic>Dehalococcoides<\/jats:italic>\n                    ' activity and growth. Guided cobalamin biosynthesis may offer opportunities to sustain and enhance\n                    <jats:italic>Dehalococcoides<\/jats:italic>\n                    activity in contaminated subsurface environments.\n                  <\/jats:p>","DOI":"10.1098\/rstb.2012.0320","type":"journal-article","created":{"date-parts":[[2013,3,11]],"date-time":"2013-03-11T22:15:04Z","timestamp":1363040104000},"page":"20120320","update-policy":"https:\/\/doi.org\/10.1098\/crossmark-policy","source":"Crossref","is-referenced-by-count":144,"title":["Guided cobalamin biosynthesis supports\n                    <i>Dehalococcoides mccartyi<\/i>\n                    reductive dechlorination activity"],"prefix":"10.1098","volume":"368","author":[{"given":"Jun","family":"Yan","sequence":"first","affiliation":[{"name":"Department of Microbiology, University of Tennessee, Knoxville, TN 37996, USA"},{"name":"Department of Civil and Environmental Engineering, University of Tennessee, Knoxville, TN 37996, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jeongdae","family":"Im","sequence":"additional","affiliation":[{"name":"Department of Microbiology, University of Tennessee, Knoxville, TN 37996, USA"},{"name":"Department of Civil and Environmental Engineering, University of Tennessee, Knoxville, TN 37996, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yi","family":"Yang","sequence":"additional","affiliation":[{"name":"Department of Civil and Environmental Engineering, University of Tennessee, Knoxville, TN 37996, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Frank E.","family":"L\u00f6ffler","sequence":"additional","affiliation":[{"name":"Department of Microbiology, University of Tennessee, Knoxville, TN 37996, USA"},{"name":"Department of Civil and Environmental Engineering, University of Tennessee, Knoxville, TN 37996, USA"},{"name":"Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, TN 37831, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"175","published-online":{"date-parts":[[2013,4,19]]},"reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.2237418"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1099\/ijs.0.034926-0"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.276.5318.1568"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1038\/35046063"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.micro.50.1.137"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1042\/bse0340139"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.69.8.4628-4638.2003"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1007\/s002030050602"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.271.28.16515"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1002\/hlca.200390313"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2164-10-78"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6968.2009.01569.x"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.02574-06"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1038\/ismej.2011.111"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.01535-12"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.2011.07741.x"},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.1515\/9783110844795"},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6968.1990.tb04936.x"},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1432-1033.1988.tb13910.x"},{"key":"e_1_3_2_21_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1432-1033.1988.tb14303.x"},{"key":"e_1_3_2_22_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature01717"},{"key":"e_1_3_2_23_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.72.3.1980-1987.2006"},{"key":"e_1_3_2_24_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1462-2920.2005.00830.x"},{"key":"e_1_3_2_25_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.72.4.2765-2774.2006"},{"key":"e_1_3_2_26_2","doi-asserted-by":"publisher","DOI":"10.1016\/0003-2697(86)90447-1"},{"key":"e_1_3_2_27_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1432-1033.1989.tb15256.x"},{"key":"e_1_3_2_28_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.tet.2007.08.061"},{"key":"e_1_3_2_29_2","doi-asserted-by":"crossref","first-page":"1347","DOI":"10.1016\/S0021-9258(17)34873-1","article-title":"Transport of vitamin B12 in Escherichia coli. 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