{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,12]],"date-time":"2026-09-12T02:39:59Z","timestamp":1789180799666,"version":"build-2803163510"},"reference-count":48,"publisher":"The Royal Society","issue":"1616","license":[{"start":{"date-parts":[[2013,4,19]],"date-time":"2013-04-19T00:00:00Z","timestamp":1366329600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/royalsociety.org\/journals\/ethics-policies\/data-sharing-mining\/"}],"content-domain":{"domain":["royalsocietypublishing.org"],"crossmark-restriction":true},"short-container-title":["Phil. Trans. R. Soc. B"],"published-print":{"date-parts":[[2013,4,19]]},"abstract":"<jats:p>\n                    The genome sequence of psychrophilic\n                    <jats:italic>Shewanella sediminis<\/jats:italic>\n                    revealed the presence of five putative reductive dehalogenases (Rdhs). We found that cell extracts of pyruvate\/fumarate-grown\n                    <jats:italic>S. sediminis<\/jats:italic>\n                    cells catalysed reduced methyl viologen-dependent reductive dechlorination of tetrachloroethene (PCE) to trichloroethene (TCE) at a specific activity of approximately 1 nmol TCE min\n                    <jats:sup>\u22121<\/jats:sup>\n                    (mg protein)\n                    <jats:sup>\u22121<\/jats:sup>\n                    . Dechlorination of PCE followed Michaelis\u2013Menten kinetics with an apparent\n                    <jats:italic>K<\/jats:italic>\n                    <jats:sub>m<\/jats:sub>\n                    of 120 \u03bcM PCE. No PCE dechlorination was observed with heat-denatured extract or when cyanocobalamin was omitted from the growth medium; however, the presence of PCE in the growth medium increased PCE transformation rates. Analysis of mutants carrying in-frame deletions of all five Rdhs encoding genes showed that only deletion of Ssed_3769 resulted in the loss of PCE dechlorination activity suggesting that Ssed_3769 is a functional Rdh. This is the first study to show reductive dechlorination activity of PCE in a sediment-dwelling\n                    <jats:italic>Shewanella<\/jats:italic>\n                    species that may be important for linking the flux of organohalogens to organic carbon via reductive dehalogenation in marine sediments.\n                  <\/jats:p>","DOI":"10.1098\/rstb.2012.0326","type":"journal-article","created":{"date-parts":[[2013,3,11]],"date-time":"2013-03-11T22:15:04Z","timestamp":1363040104000},"page":"20120326","update-policy":"https:\/\/doi.org\/10.1098\/crossmark-policy","source":"Crossref","is-referenced-by-count":39,"title":["Identification of a reductive tetrachloroethene dehalogenase in\n                    <i>Shewanella sediminis<\/i>"],"prefix":"10.1098","volume":"368","author":[{"given":"Svenja T.","family":"Lohner","sequence":"first","affiliation":[{"name":"Civil and Environmental Engineering, Stanford University, Stanford, CA 94305, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alfred M.","family":"Spormann","sequence":"additional","affiliation":[{"name":"Department of Chemical Engineering, Stanford University, Stanford, CA 94305, USA"},{"name":"Civil and Environmental Engineering, Stanford University, Stanford, CA 94305, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"175","published-online":{"date-parts":[[2013,4,19]]},"reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.micro.58.030603.123600"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF02818543"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.4056\/sigs.2806097"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1102226"},{"key":"e_1_3_2_6_2","doi-asserted-by":"crossref","first-page":"482","DOI":"10.1128\/MR.56.3.482-507.1992","article-title":"Microbial reductive dehalogenation","volume":"56","author":"Mohn W","year":"1992","journal-title":"Microbiol. 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