{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,27]],"date-time":"2026-09-27T16:01:08Z","timestamp":1790524868209,"version":"4.1.0"},"reference-count":47,"publisher":"The Royal Society","issue":"1654","license":[{"start":{"date-parts":[[2014,10,19]],"date-time":"2014-10-19T00:00:00Z","timestamp":1413676800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/royalsociety.org\/journals\/ethics-policies\/data-sharing-mining\/"}],"content-domain":{"domain":["royalsocietypublishing.org"],"crossmark-restriction":true},"short-container-title":["Phil. Trans. R. Soc. B"],"published-print":{"date-parts":[[2014,10,19]]},"abstract":"<jats:p>\n                    Perineuronal nets (PNs) in the brains of tenascin-R-deficient (\n                    <jats:italic>tn-r<\/jats:italic>\n                    <jats:sup>\u2212\/\u2212<\/jats:sup>\n                    ) mice develop in temporal concordance with those of wild-type (\n                    <jats:italic>\n                      tn-r\n                      <jats:sup>+\/+<\/jats:sup>\n                    <\/jats:italic>\n                    ) mice. However, the histological appearance of PNs is abnormal in adult\n                    <jats:italic>tn-r<\/jats:italic>\n                    <jats:sup>\u2212\/\u2212<\/jats:sup>\n                    mice. Here, we investigated whether similar defects are also seen in dissociated and organotypic cultures from hippocampus and forebrain of\n                    <jats:italic>tn-r<\/jats:italic>\n                    <jats:sup>\u2212\/\u2212<\/jats:sup>\n                    mice and whether the structure of PNs could be normalized. In\n                    <jats:italic>tn-r<\/jats:italic>\n                    <jats:sup>\u2212\/\u2212<\/jats:sup>\n                    cultures, accumulations of several extracellular matrix molecules were mostly associated with somata, whereas dendrites were sparsely covered, compared with\n                    <jats:italic>\n                      tn-r\n                      <jats:sup>+\/+<\/jats:sup>\n                    <\/jats:italic>\n                    mice. Experiments to normalize the structure of PNs in\n                    <jats:italic>tn-r<\/jats:italic>\n                    <jats:sup>\u2212\/\u2212<\/jats:sup>\n                    organotypic slice cultures by depolarization of neurons, or by co-culturing\n                    <jats:italic>tn-r<\/jats:italic>\n                    <jats:sup>+\/+<\/jats:sup>\n                    and\n                    <jats:italic>tn-r<\/jats:italic>\n                    <jats:sup>\u2212\/\u2212<\/jats:sup>\n                    brain slices failed to restore a normal PN phenotype. However, formation of dendritic PNs in cultures was improved by the application of tenascin-R protein and rescued by polyclonal antibodies to aggrecan and a bivalent, but not monovalent form of the lectin\n                    <jats:italic>Wisteria floribunda<\/jats:italic>\n                    agglutinin. These results show that tenascin-R and aggrecan are decisive contributors to formation and stabilization of PNs and that tenascin-R may implement these functions by clustering of aggrecan. Proposed approaches for restoration of normal PN structure are noteworthy in the context of PN abnormalities in neurological disorders, such as epilepsy, schizophrenia and addiction.\n                  <\/jats:p>","DOI":"10.1098\/rstb.2014.0046","type":"journal-article","created":{"date-parts":[[2014,9,16]],"date-time":"2014-09-16T01:50:39Z","timestamp":1410832239000},"page":"20140046","update-policy":"https:\/\/doi.org\/10.1098\/crossmark-policy","source":"Crossref","is-referenced-by-count":104,"title":["Tenascin-R promotes assembly of the extracellular matrix of perineuronal nets via clustering of aggrecan"],"prefix":"10.1098","volume":"369","author":[{"given":"Markus","family":"Morawski","sequence":"first","affiliation":[{"name":"Paul Flechsig Institute for Brain Research, University of Leipzig, Jahnallee 59, 04109 Leipzig, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alexander","family":"Dityatev","sequence":"additional","affiliation":[{"name":"Zentrum f\u00fcr Molekulare Neurobiologie, Universit\u00e4tsklinikum Hamburg - Eppendorf, Martinistr. 52, 20246 Hamburg, Germany"},{"name":"Department of Neuroscience and Brain Technologies, Italian Institute of Technology, via Morego 30, Genoa, Italy"},{"name":"Molecular Neuroplasticity Group, German Center for Neurodegenerative Diseases (DZNE), 39120 Magdeburg, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maike","family":"Hartlage-R\u00fcbsamen","sequence":"additional","affiliation":[{"name":"Paul Flechsig Institute for Brain Research, University of Leipzig, Jahnallee 59, 04109 Leipzig, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maren","family":"Blosa","sequence":"additional","affiliation":[{"name":"Paul Flechsig Institute for Brain Research, University of Leipzig, Jahnallee 59, 04109 Leipzig, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Max","family":"Holzer","sequence":"additional","affiliation":[{"name":"Paul Flechsig Institute for Brain Research, University of Leipzig, Jahnallee 59, 04109 Leipzig, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Katharina","family":"Flach","sequence":"additional","affiliation":[{"name":"Paul Flechsig Institute for Brain Research, University of Leipzig, Jahnallee 59, 04109 Leipzig, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sanja","family":"Pavlica","sequence":"additional","affiliation":[{"name":"Paul Flechsig Institute for Brain Research, University of Leipzig, Jahnallee 59, 04109 Leipzig, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Galina","family":"Dityateva","sequence":"additional","affiliation":[{"name":"Zentrum f\u00fcr Molekulare Neurobiologie, Universit\u00e4tsklinikum Hamburg - 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