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Trans. R. Soc. B"],"published-print":{"date-parts":[[2022,11,21]]},"abstract":"<jats:p>The highly organized transverse tubule (t-tubule) network facilitates cardiac excitation\u2013contraction coupling and synchronous cardiac myocyte contraction. In cardiac failure secondary to myocardial infarction (MI), changes in the structure and organization of t-tubules result in impaired cardiac contractility. However, there is still little knowledge on the regional variation of t-tubule remodelling in cardiac failure post-MI. Here, we investigate post-MI t-tubule remodelling in infarct border and remote regions, using serial block face scanning electron microscopy (SBF-SEM) applied to a translationally relevant sheep ischaemia reperfusion MI model and matched controls. We performed minimally invasive coronary angioplasty of the left anterior descending artery, followed by reperfusion after 90 min to establish the MI model. Left ventricular tissues obtained from control and MI hearts eight weeks post-MI were imaged using SBF-SEM. Image analysis generated three-dimensional reconstructions of the t-tubular network in control, MI border and remote regions. Quantitative analysis revealed that the MI border region was characterized by t-tubule depletion and fragmentation, dilation of surviving t-tubules and t-tubule elongation. This study highlights region-dependent remodelling of the tubular network post-MI and may provide novel localized therapeutic targets aimed at preservation or restoration of the t-tubules to manage cardiac contractility post-MI.<\/jats:p>\n                  <jats:p>This article is part of the theme issue \u2018The cardiomyocyte: new revelations on the interplay between architecture and function in growth, health, and disease\u2019.<\/jats:p>","DOI":"10.1098\/rstb.2021.0331","type":"journal-article","created":{"date-parts":[[2022,10,3]],"date-time":"2022-10-03T06:18:23Z","timestamp":1664777903000},"update-policy":"https:\/\/doi.org\/10.1098\/crossmark-policy","source":"Crossref","is-referenced-by-count":8,"title":["Serial block face scanning electron microscopy reveals region-dependent remodelling of transverse tubules post-myocardial infarction"],"prefix":"10.1098","volume":"377","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9627-9602","authenticated-orcid":true,"given":"Tharushi","family":"Perera","sequence":"first","affiliation":[{"name":"Unit of Cardiac Physiology, Division of Cardiovascular Sciences, Faculty of Biology Medicine and Health, University of Manchester, Manchester Academic Health Sciences Centre, 46 Grafton Street, Manchester M13 9NT, UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Charlene","family":"Pius","sequence":"additional","affiliation":[{"name":"Unit of Cardiac Physiology, Division of Cardiovascular Sciences, Faculty of Biology Medicine and Health, University of Manchester, Manchester Academic Health Sciences Centre, 46 Grafton Street, Manchester M13 9NT, UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9328-6452","authenticated-orcid":true,"given":"Barbara","family":"Niort","sequence":"additional","affiliation":[{"name":"Unit of Cardiac Physiology, Division of Cardiovascular Sciences, Faculty of Biology Medicine and Health, University of Manchester, Manchester Academic Health Sciences Centre, 46 Grafton Street, Manchester M13 9NT, UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8560-4812","authenticated-orcid":true,"given":"Emma J.","family":"Radcliffe","sequence":"additional","affiliation":[{"name":"Unit of Cardiac Physiology, Division of Cardiovascular Sciences, Faculty of Biology Medicine and Health, University of Manchester, Manchester Academic Health Sciences Centre, 46 Grafton Street, Manchester M13 9NT, UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9309-3853","authenticated-orcid":true,"given":"Katharine M.","family":"Dibb","sequence":"additional","affiliation":[{"name":"Unit of Cardiac Physiology, Division of Cardiovascular Sciences, Faculty of Biology Medicine and Health, University of Manchester, Manchester Academic Health Sciences Centre, 46 Grafton Street, Manchester M13 9NT, UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Andrew W.","family":"Trafford","sequence":"additional","affiliation":[{"name":"Unit of Cardiac Physiology, Division of Cardiovascular Sciences, Faculty of Biology Medicine and Health, University of Manchester, Manchester Academic Health Sciences Centre, 46 Grafton Street, Manchester M13 9NT, UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9815-6940","authenticated-orcid":true,"given":"Christian","family":"Pinali","sequence":"additional","affiliation":[{"name":"Unit of Cardiac Physiology, Division of Cardiovascular Sciences, Faculty of Biology Medicine and Health, University of Manchester, Manchester Academic Health Sciences Centre, 46 Grafton Street, Manchester M13 9NT, UK"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"175","published-online":{"date-parts":[[2022,10,3]]},"reference":[{"key":"e_1_3_7_2_2","doi-asserted-by":"publisher","DOI":"10.1038\/415198a"},{"key":"e_1_3_7_3_2","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.2006.107227"},{"key":"e_1_3_7_4_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0017901"},{"key":"e_1_3_7_5_2","doi-asserted-by":"publisher","DOI":"10.1007\/s10974-006-9089-y"},{"key":"e_1_3_7_6_2","doi-asserted-by":"publisher","DOI":"10.1152\/physrev.00037.2015"},{"key":"e_1_3_7_7_2","first-page":"62","article-title":"Cardiac remodeling: concepts, clinical impact, pathophysiological mechanisms and pharmacologic treatment","volume":"106","author":"Azevedo PS","year":"2016","unstructured":"Azevedo PS, Polegato BF, Minicucci MF, Paiva SA, Zornoff LA. 2016 Cardiac remodeling: concepts, clinical impact, pathophysiological mechanisms and pharmacologic treatment. 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